Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistan...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.631716/full |
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| author | Mathilde L. Pas Kevin Vanneste Jade Bokma Laura Van Driessche Sigrid C. J. De Keersmaecker Nancy H. Roosens Freddy Haesebrouck Filip Boyen Bart Pardon |
| author_facet | Mathilde L. Pas Kevin Vanneste Jade Bokma Laura Van Driessche Sigrid C. J. De Keersmaecker Nancy H. Roosens Freddy Haesebrouck Filip Boyen Bart Pardon |
| author_sort | Mathilde L. Pas |
| collection | DOAJ |
| description | Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistant Raoultella ornithinolytica, an emerging pathogen in human medicine, in a calf with suspected sepsis. R. ornithinolytica was identified by MALDI-TOF MS from blood cultures of a critically ill calf. Susceptibility testing showed phenotypic resistance against ampicillin, gentamicin, potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as R. ornithinolytica and the multidrug resistant character of the isolate. Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical parenteral therapy with enrofloxacin and sodium penicillin for seven days. Ancillary therapy consisted of fluid therapy, ketoprofen and doxapram hydrochloride. To the authors' knowledge, this is the first report characterizing a multidrug resistant R. ornithinolytica isolate from blood culture in cattle. It is currently unknown whether animals and farms may act as reservoirs for multidrug resistant R. ornithinolytica strains. |
| first_indexed | 2024-12-22T16:56:22Z |
| format | Article |
| id | doaj.art-31ce3d3f3aee4d0889c1ad85c784e995 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-22T16:56:22Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-31ce3d3f3aee4d0889c1ad85c784e9952022-12-21T18:19:27ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-03-01810.3389/fvets.2021.631716631716Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic CalfMathilde L. Pas0Kevin Vanneste1Jade Bokma2Laura Van Driessche3Sigrid C. J. De Keersmaecker4Nancy H. Roosens5Freddy Haesebrouck6Filip Boyen7Bart Pardon8Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumSciensano, Transversal Activities in Applied Genomics, Brussels, BelgiumDepartment of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumDepartment of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumSciensano, Transversal Activities in Applied Genomics, Brussels, BelgiumSciensano, Transversal Activities in Applied Genomics, Brussels, BelgiumDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumDepartment of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumSepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistant Raoultella ornithinolytica, an emerging pathogen in human medicine, in a calf with suspected sepsis. R. ornithinolytica was identified by MALDI-TOF MS from blood cultures of a critically ill calf. Susceptibility testing showed phenotypic resistance against ampicillin, gentamicin, potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as R. ornithinolytica and the multidrug resistant character of the isolate. Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical parenteral therapy with enrofloxacin and sodium penicillin for seven days. Ancillary therapy consisted of fluid therapy, ketoprofen and doxapram hydrochloride. To the authors' knowledge, this is the first report characterizing a multidrug resistant R. ornithinolytica isolate from blood culture in cattle. It is currently unknown whether animals and farms may act as reservoirs for multidrug resistant R. ornithinolytica strains.https://www.frontiersin.org/articles/10.3389/fvets.2021.631716/fullantimicrobial resistanceEnterobacteriaceaecase reportcattlesepsiswhole genome sequencing |
| spellingShingle | Mathilde L. Pas Kevin Vanneste Jade Bokma Laura Van Driessche Sigrid C. J. De Keersmaecker Nancy H. Roosens Freddy Haesebrouck Filip Boyen Bart Pardon Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf Frontiers in Veterinary Science antimicrobial resistance Enterobacteriaceae case report cattle sepsis whole genome sequencing |
| title | Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf |
| title_full | Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf |
| title_fullStr | Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf |
| title_full_unstemmed | Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf |
| title_short | Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf |
| title_sort | case report multidrug resistant raoultella ornithinolytica in a septicemic calf |
| topic | antimicrobial resistance Enterobacteriaceae case report cattle sepsis whole genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.631716/full |
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