Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis.
Natronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH. It has become one of the model organisms for the study of extremophilic life. Here, we present a genome-scale, manually curated metabolic reconstruction for the microorganism. The recons...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-06-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC2881530?pdf=render |
| _version_ | 1818583385681952768 |
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| author | Orland Gonzalez Tanja Oberwinkler Locedie Mansueto Friedhelm Pfeiffer Eduardo Mendoza Ralf Zimmer Dieter Oesterhelt |
| author_facet | Orland Gonzalez Tanja Oberwinkler Locedie Mansueto Friedhelm Pfeiffer Eduardo Mendoza Ralf Zimmer Dieter Oesterhelt |
| author_sort | Orland Gonzalez |
| collection | DOAJ |
| description | Natronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH. It has become one of the model organisms for the study of extremophilic life. Here, we present a genome-scale, manually curated metabolic reconstruction for the microorganism. The reconstruction itself represents a knowledge base of the haloalkaliphile's metabolism and, as such, would greatly assist further investigations on archaeal pathways. In addition, we experimentally determined several parameters relevant to growth, including a characterization of the biomass composition and a quantification of carbon and oxygen consumption. Using the metabolic reconstruction and the experimental data, we formulated a constraints-based model which we used to analyze the behavior of the archaeon when grown on a single carbon source. Results of the analysis include the finding that Natronomonas pharaonis, when grown aerobically on acetate, uses a carbon to oxygen consumption ratio that is theoretically near-optimal with respect to growth and energy production. This supports the hypothesis that, under simple conditions, the microorganism optimizes its metabolism with respect to the two objectives. We also found that the archaeon has a very low carbon efficiency of only about 35%. This inefficiency is probably due to a very low P/O ratio as well as to the other difficulties posed by its extreme environment. |
| first_indexed | 2024-12-16T08:04:27Z |
| format | Article |
| id | doaj.art-31d299d1ed604ca59de6310004bbb31b |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-16T08:04:27Z |
| publishDate | 2010-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-31d299d1ed604ca59de6310004bbb31b2022-12-21T22:38:30ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582010-06-0166e100079910.1371/journal.pcbi.1000799Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis.Orland GonzalezTanja OberwinklerLocedie MansuetoFriedhelm PfeifferEduardo MendozaRalf ZimmerDieter OesterheltNatronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH. It has become one of the model organisms for the study of extremophilic life. Here, we present a genome-scale, manually curated metabolic reconstruction for the microorganism. The reconstruction itself represents a knowledge base of the haloalkaliphile's metabolism and, as such, would greatly assist further investigations on archaeal pathways. In addition, we experimentally determined several parameters relevant to growth, including a characterization of the biomass composition and a quantification of carbon and oxygen consumption. Using the metabolic reconstruction and the experimental data, we formulated a constraints-based model which we used to analyze the behavior of the archaeon when grown on a single carbon source. Results of the analysis include the finding that Natronomonas pharaonis, when grown aerobically on acetate, uses a carbon to oxygen consumption ratio that is theoretically near-optimal with respect to growth and energy production. This supports the hypothesis that, under simple conditions, the microorganism optimizes its metabolism with respect to the two objectives. We also found that the archaeon has a very low carbon efficiency of only about 35%. This inefficiency is probably due to a very low P/O ratio as well as to the other difficulties posed by its extreme environment.http://europepmc.org/articles/PMC2881530?pdf=render |
| spellingShingle | Orland Gonzalez Tanja Oberwinkler Locedie Mansueto Friedhelm Pfeiffer Eduardo Mendoza Ralf Zimmer Dieter Oesterhelt Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. PLoS Computational Biology |
| title | Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. |
| title_full | Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. |
| title_fullStr | Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. |
| title_full_unstemmed | Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. |
| title_short | Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis. |
| title_sort | characterization of growth and metabolism of the haloalkaliphile natronomonas pharaonis |
| url | http://europepmc.org/articles/PMC2881530?pdf=render |
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