Self-assembly of silver nanoparticles and bacteriophage
Biohybrid nanostructured materials, composed of both inorganic nanoparticles and biomolecules, offer prospects for many new applications in extremely diverse fields such as chemistry, physics, engineering, medicine and nanobiotechnology. In the recent years, Phage display technique has been extensiv...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Sensing and Bio-Sensing Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180416300149 |
| _version_ | 1819153674013769728 |
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| author | Santi Scibilia Germana Lentini Enza Fazio Domenico Franco Fortunato Neri Angela Maria Mezzasalma Salvatore Pietro Paolo Guglielmino |
| author_facet | Santi Scibilia Germana Lentini Enza Fazio Domenico Franco Fortunato Neri Angela Maria Mezzasalma Salvatore Pietro Paolo Guglielmino |
| author_sort | Santi Scibilia |
| collection | DOAJ |
| description | Biohybrid nanostructured materials, composed of both inorganic nanoparticles and biomolecules, offer prospects for many new applications in extremely diverse fields such as chemistry, physics, engineering, medicine and nanobiotechnology. In the recent years, Phage display technique has been extensively used to generate phage clones displaying surface peptides with functionality towards organic materials. Screening and selection of phage displayed material binding peptides has attracted great interest because of their use for development of hybrid materials with multiple functionalities. Here, we present a self-assembly approach for the construction of hybrid nanostructured networks consisting of M13 P9b phage clone, specific for Pseudomonas aeruginosa, selected by Phage display technology, directly assembled with silver nanoparticles (AgNPs), previously prepared by pulsed laser ablation. These networks are characterized by UV–vis optical spectroscopy, scanning/transmission electron microscopies and Raman spectroscopy. We investigated the influence of different ions and medium pH on self-assembly by evaluating different phage suspension buffers. The assembly of these networks is controlled by electrostatic interactions between the phage pVIII major capsid proteins and the AgNPs. The formation of the AgNPs-phage networks was obtained only in two types of tested buffers at a pH value near the isoelectric point of each pVIII proteins displayed on the surface of the clone. This systematic study allowed to optimize the synthesis procedure to assembly AgNPs and bacteriophage. Such networks find application in the biomedical field of advanced biosensing and targeted gene and drug delivery. Keywords: Phage display, Silver nanoparticles, Self-assembly, Hybrid architecture, Raman spectroscopy |
| first_indexed | 2024-12-22T15:08:56Z |
| format | Article |
| id | doaj.art-31d49d3590a743ae817e8170f3f285da |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-12-22T15:08:56Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-31d49d3590a743ae817e8170f3f285da2022-12-21T18:21:54ZengElsevierSensing and Bio-Sensing Research2214-18042016-03-017146152Self-assembly of silver nanoparticles and bacteriophageSanti Scibilia0Germana Lentini1Enza Fazio2Domenico Franco3Fortunato Neri4Angela Maria Mezzasalma5Salvatore Pietro Paolo Guglielmino6Department of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences (MIFT), Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy; Corresponding authors.Department of Biological and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, ItalyDepartment of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences (MIFT), Viale F. Stagno d'Alcontres 31, 98166 Messina, ItalyDepartment of Biological and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, ItalyDepartment of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences (MIFT), Viale F. Stagno d'Alcontres 31, 98166 Messina, ItalyDepartment of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences (MIFT), Viale F. Stagno d'Alcontres 31, 98166 Messina, ItalyDepartment of Biological and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy; Corresponding authors.Biohybrid nanostructured materials, composed of both inorganic nanoparticles and biomolecules, offer prospects for many new applications in extremely diverse fields such as chemistry, physics, engineering, medicine and nanobiotechnology. In the recent years, Phage display technique has been extensively used to generate phage clones displaying surface peptides with functionality towards organic materials. Screening and selection of phage displayed material binding peptides has attracted great interest because of their use for development of hybrid materials with multiple functionalities. Here, we present a self-assembly approach for the construction of hybrid nanostructured networks consisting of M13 P9b phage clone, specific for Pseudomonas aeruginosa, selected by Phage display technology, directly assembled with silver nanoparticles (AgNPs), previously prepared by pulsed laser ablation. These networks are characterized by UV–vis optical spectroscopy, scanning/transmission electron microscopies and Raman spectroscopy. We investigated the influence of different ions and medium pH on self-assembly by evaluating different phage suspension buffers. The assembly of these networks is controlled by electrostatic interactions between the phage pVIII major capsid proteins and the AgNPs. The formation of the AgNPs-phage networks was obtained only in two types of tested buffers at a pH value near the isoelectric point of each pVIII proteins displayed on the surface of the clone. This systematic study allowed to optimize the synthesis procedure to assembly AgNPs and bacteriophage. Such networks find application in the biomedical field of advanced biosensing and targeted gene and drug delivery. Keywords: Phage display, Silver nanoparticles, Self-assembly, Hybrid architecture, Raman spectroscopyhttp://www.sciencedirect.com/science/article/pii/S2214180416300149 |
| spellingShingle | Santi Scibilia Germana Lentini Enza Fazio Domenico Franco Fortunato Neri Angela Maria Mezzasalma Salvatore Pietro Paolo Guglielmino Self-assembly of silver nanoparticles and bacteriophage Sensing and Bio-Sensing Research |
| title | Self-assembly of silver nanoparticles and bacteriophage |
| title_full | Self-assembly of silver nanoparticles and bacteriophage |
| title_fullStr | Self-assembly of silver nanoparticles and bacteriophage |
| title_full_unstemmed | Self-assembly of silver nanoparticles and bacteriophage |
| title_short | Self-assembly of silver nanoparticles and bacteriophage |
| title_sort | self assembly of silver nanoparticles and bacteriophage |
| url | http://www.sciencedirect.com/science/article/pii/S2214180416300149 |
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