Cytotoxic effects of delphinidin in human osteosarcoma cells
Introduction: The aim of this study was to evaluate whether delphinidin is cytoprotective or cytotoxic in osteosarcoma cell lines, and to elucidate the underlying mechanisms. Materials and methods: The present study investigated whether apoptosis or autophagy is induced by delphinidin in human osteo...
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| Format: | Article |
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AVES
2018-01-01
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| Series: | Acta Orthopaedica et Traumatologica Turcica |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1017995X17303322 |
| _version_ | 1797910356451917824 |
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| author | Dong-Yeong Lee Young-Jin Park Sun-Chul Hwang Kwang-Dong Kim Dong-Kyu Moon Dong-Hee Kim |
| author_facet | Dong-Yeong Lee Young-Jin Park Sun-Chul Hwang Kwang-Dong Kim Dong-Kyu Moon Dong-Hee Kim |
| author_sort | Dong-Yeong Lee |
| collection | DOAJ |
| description | Introduction: The aim of this study was to evaluate whether delphinidin is cytoprotective or cytotoxic in osteosarcoma cell lines, and to elucidate the underlying mechanisms. Materials and methods: The present study investigated whether apoptosis or autophagy is induced by delphinidin in human osteosarcoma cell lines. Delphinidin was used as the antioxidant, along with two autophagy inhibitors: 3-methyladenine and bafilomycin A1. Cell viability and known autophagic markers, such as LC3-II expression, were evaluated. Reactive oxygen species (ROS) formation and cell cycle analysis were also investigated. Results: Delphinidin showed concentration-dependent cytotoxicity to osteosarcoma cell. Delphinidin is closely associated with apoptotic cell death mechanisms and pathways related to ROS accumulation. In addition, we observed delphinidin-induced autophagosome formation and increasing levels of LC3-II conversion. However, in spite of delphinidin induced autophagy, the cytotoxic effects induced in the osteosarcoma cells may not be operating via autophagic cell death mechanisms. Conclusions: Delphinidin compromises the cellular protective mechanisms by inhibiting autophagy, permitting ROS to accumulate and finally enhance apoptotic cell death. Our results indicate that delphinidin may play a critical role as a chemotherapeutic agent by preventing the development and progression of osteosarcoma cells. Keywords: Delphinidin, Cytotoxicity, Autophagy, Apoptosis, Osteosarcoma |
| first_indexed | 2024-04-10T11:23:04Z |
| format | Article |
| id | doaj.art-31d6e88d9caf45fbaf524a17f3627a4b |
| institution | Directory Open Access Journal |
| issn | 1017-995X |
| language | English |
| last_indexed | 2024-04-10T11:23:04Z |
| publishDate | 2018-01-01 |
| publisher | AVES |
| record_format | Article |
| series | Acta Orthopaedica et Traumatologica Turcica |
| spelling | doaj.art-31d6e88d9caf45fbaf524a17f3627a4b2023-02-15T16:18:31ZengAVESActa Orthopaedica et Traumatologica Turcica1017-995X2018-01-015215864Cytotoxic effects of delphinidin in human osteosarcoma cellsDong-Yeong Lee0Young-Jin Park1Sun-Chul Hwang2Kwang-Dong Kim3Dong-Kyu Moon4Dong-Hee Kim5Armed Forces Deagu Hospital, Daegu, Republic of Korea; Department of Orthopaedic Surgery and Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Republic of KoreaDepartment of Orthopaedic Surgery and Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Republic of KoreaDepartment of Orthopaedic Surgery and Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Republic of KoreaDivision of Applied Life Science, Gyeongsang National University, Jinju, Republic of KoreaDepartment of Orthopaedic Surgery and Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Republic of KoreaDepartment of Orthopaedic Surgery and Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Republic of Korea; Corresponding author. Department of Orthopaedic Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, 15, Jinju-daero 816 beon-gil, Jinju-si, Gyeongsangnam-do, 660-751, Republic of Korea. Fax: +82 55 761 9477.Introduction: The aim of this study was to evaluate whether delphinidin is cytoprotective or cytotoxic in osteosarcoma cell lines, and to elucidate the underlying mechanisms. Materials and methods: The present study investigated whether apoptosis or autophagy is induced by delphinidin in human osteosarcoma cell lines. Delphinidin was used as the antioxidant, along with two autophagy inhibitors: 3-methyladenine and bafilomycin A1. Cell viability and known autophagic markers, such as LC3-II expression, were evaluated. Reactive oxygen species (ROS) formation and cell cycle analysis were also investigated. Results: Delphinidin showed concentration-dependent cytotoxicity to osteosarcoma cell. Delphinidin is closely associated with apoptotic cell death mechanisms and pathways related to ROS accumulation. In addition, we observed delphinidin-induced autophagosome formation and increasing levels of LC3-II conversion. However, in spite of delphinidin induced autophagy, the cytotoxic effects induced in the osteosarcoma cells may not be operating via autophagic cell death mechanisms. Conclusions: Delphinidin compromises the cellular protective mechanisms by inhibiting autophagy, permitting ROS to accumulate and finally enhance apoptotic cell death. Our results indicate that delphinidin may play a critical role as a chemotherapeutic agent by preventing the development and progression of osteosarcoma cells. Keywords: Delphinidin, Cytotoxicity, Autophagy, Apoptosis, Osteosarcomahttp://www.sciencedirect.com/science/article/pii/S1017995X17303322 |
| spellingShingle | Dong-Yeong Lee Young-Jin Park Sun-Chul Hwang Kwang-Dong Kim Dong-Kyu Moon Dong-Hee Kim Cytotoxic effects of delphinidin in human osteosarcoma cells Acta Orthopaedica et Traumatologica Turcica |
| title | Cytotoxic effects of delphinidin in human osteosarcoma cells |
| title_full | Cytotoxic effects of delphinidin in human osteosarcoma cells |
| title_fullStr | Cytotoxic effects of delphinidin in human osteosarcoma cells |
| title_full_unstemmed | Cytotoxic effects of delphinidin in human osteosarcoma cells |
| title_short | Cytotoxic effects of delphinidin in human osteosarcoma cells |
| title_sort | cytotoxic effects of delphinidin in human osteosarcoma cells |
| url | http://www.sciencedirect.com/science/article/pii/S1017995X17303322 |
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