DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming.
In this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy-phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-02-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2817727?pdf=render |
| _version_ | 1818097322129620992 |
|---|---|
| author | Min Ki Jee Ji Hoon Kim Yong Man Han Sung Jun Jung Kyung Sun Kang Dong Wook Kim Soo Kyung Kang |
| author_facet | Min Ki Jee Ji Hoon Kim Yong Man Han Sung Jun Jung Kyung Sun Kang Dong Wook Kim Soo Kyung Kang |
| author_sort | Min Ki Jee |
| collection | DOAJ |
| description | In this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy-phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-1, Oct-4, Sox-2, and Nanog. Additionally, demethylation of the regulatory regions of Rex-1, stemnesses, and HIF1alpha and scavenging of reactive oxygen species were finally resulted in an improved stem cell behavior of de-differentiate ATSC (de-ATSC). Proliferation activity of ATSCs after dedifferentiation was induced by REX1, Oct4, and JAK/STAT3 directly or indirectly. De-ATSCs showed increased migration activity that mediated by P38/JUNK and ERK phosphorylation. Moreover, regenerative efficacy of de-ATSC engrafted spinal cord-injured rats and chemical-induced diabetes animals were significantly restored their functions.Our stem cell remodeling system may provide a good model which would provide insight into the molecular mechanisms underlying ATSC proliferation and transdifferentiation. Also, these multipotent stem cells can be harvested may provide us with a valuable reservoir of primitive and autologous stem cells for use in a broad spectrum of regenerative cell-based disease therapy. |
| first_indexed | 2024-12-10T23:18:40Z |
| format | Article |
| id | doaj.art-31e18c73abcc4a9ea1da5679a7ee2b70 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T23:18:40Z |
| publishDate | 2010-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-31e18c73abcc4a9ea1da5679a7ee2b702022-12-22T01:29:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-02-0152e902610.1371/journal.pone.0009026DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming.Min Ki JeeJi Hoon KimYong Man HanSung Jun JungKyung Sun KangDong Wook KimSoo Kyung KangIn this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy-phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-1, Oct-4, Sox-2, and Nanog. Additionally, demethylation of the regulatory regions of Rex-1, stemnesses, and HIF1alpha and scavenging of reactive oxygen species were finally resulted in an improved stem cell behavior of de-differentiate ATSC (de-ATSC). Proliferation activity of ATSCs after dedifferentiation was induced by REX1, Oct4, and JAK/STAT3 directly or indirectly. De-ATSCs showed increased migration activity that mediated by P38/JUNK and ERK phosphorylation. Moreover, regenerative efficacy of de-ATSC engrafted spinal cord-injured rats and chemical-induced diabetes animals were significantly restored their functions.Our stem cell remodeling system may provide a good model which would provide insight into the molecular mechanisms underlying ATSC proliferation and transdifferentiation. Also, these multipotent stem cells can be harvested may provide us with a valuable reservoir of primitive and autologous stem cells for use in a broad spectrum of regenerative cell-based disease therapy.http://europepmc.org/articles/PMC2817727?pdf=render |
| spellingShingle | Min Ki Jee Ji Hoon Kim Yong Man Han Sung Jun Jung Kyung Sun Kang Dong Wook Kim Soo Kyung Kang DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. PLoS ONE |
| title | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. |
| title_full | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. |
| title_fullStr | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. |
| title_full_unstemmed | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. |
| title_short | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming. |
| title_sort | dhp derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming |
| url | http://europepmc.org/articles/PMC2817727?pdf=render |
| work_keys_str_mv | AT minkijee dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT jihoonkim dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT yongmanhan dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT sungjunjung dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT kyungsunkang dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT dongwookkim dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming AT sookyungkang dhpderivativeandlowoxygentensioneffectivelyinduceshumanadiposestromalcellreprogramming |