Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model
Aim of the study Biologic reconstruction using tumor-bearing bone autografts devitalized by liquid nitrogen or extracorporeal irradiation (oncological sterilization) is a safe and effective method in musculoskeletal surgery. The purpose of this study was to examine the effects of these two oncologic...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-08-01
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| Series: | Journal of Investigative Surgery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08941939.2019.1691686 |
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| author | Hüseyin Kaya Dündar Sabah Burçin Keçeci Levent Küçük Oytun Erbaş Fatih Oltulu Gürkan Yiğittürk Dilek Taskiran |
| author_facet | Hüseyin Kaya Dündar Sabah Burçin Keçeci Levent Küçük Oytun Erbaş Fatih Oltulu Gürkan Yiğittürk Dilek Taskiran |
| author_sort | Hüseyin Kaya |
| collection | DOAJ |
| description | Aim of the study Biologic reconstruction using tumor-bearing bone autografts devitalized by liquid nitrogen or extracorporeal irradiation (oncological sterilization) is a safe and effective method in musculoskeletal surgery. The purpose of this study was to examine the effects of these two oncological sterilization methods on nerve recovery. Methods A total of 48 rats were randomly divided into 3 groups as autograft, irradiation and liquid nitrogen groups. A nerve defect created in the right sciatic nerve was reconstructed with an autograft obtained from the nerve itself. Group I underwent reconstruction with standard nerve autograft. Group II and Group III underwent reconstruction with devitalized nerve autograft treated through extracorporeal irradiation and liquid nitrogen, respectively. The left sciatic nerves of the rats served as control. Electromyography, motor function test and histomorphological analysis were performed to assess the nerve recovery on the 3rd (early stage) and 4th months (late stage). Results Electrophysiological assessment revealed better results in irradiation group compared with liquid nitrogen group in terms of myelinization and axonal regeneration. Motor performance of the autograft group was slightly better than the other groups. Histologically, autograft group demonstrated better results compared with other groups. Late-stage assessments revealed high rates of myelinization in the graft segment in liquid nitrogen group and in the segment distal to the graft in irradiation group. Conclusions This study has demonstrated that nerve autografts treated by oncological sterilization methods may be used for nerve reconstruction in limb salvage surgery. However, further studies are needed to clarify the applicability of these methods. |
| first_indexed | 2024-03-12T00:32:54Z |
| format | Article |
| id | doaj.art-31e2c0d4a4a7471087d341d7a7621ef0 |
| institution | Directory Open Access Journal |
| issn | 0894-1939 1521-0553 |
| language | English |
| last_indexed | 2024-03-12T00:32:54Z |
| publishDate | 2021-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj.art-31e2c0d4a4a7471087d341d7a7621ef02023-09-15T10:07:31ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532021-08-0134777378310.1080/08941939.2019.16916861691686Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat ModelHüseyin Kaya0Dündar Sabah1Burçin Keçeci2Levent Küçük3Oytun Erbaş4Fatih Oltulu5Gürkan Yiğittürk6Dilek Taskiran7Department of Orthopedics and Traumatology, Faculty of Medicine, Ege UniversityDepartment of Orthopedics and Traumatology, Faculty of Medicine, Ege UniversityDepartment of Orthopedics and Traumatology, Faculty of Medicine, Ege UniversityDepartment of Orthopedics and Traumatology, Faculty of Medicine, Ege UniversityDepartment of Physiology, Faculty of Medicine, Istanbul Bilim UniversityDepartment of Histology and Embryology, Faculty of Medicine, Ege UniversityDepartment of Histology and Embryology, Faculty of Medicine, Muğla Sıtkı Koçman UniversityDepartment of Physiology, Faculty of Medicine, Ege UniversityAim of the study Biologic reconstruction using tumor-bearing bone autografts devitalized by liquid nitrogen or extracorporeal irradiation (oncological sterilization) is a safe and effective method in musculoskeletal surgery. The purpose of this study was to examine the effects of these two oncological sterilization methods on nerve recovery. Methods A total of 48 rats were randomly divided into 3 groups as autograft, irradiation and liquid nitrogen groups. A nerve defect created in the right sciatic nerve was reconstructed with an autograft obtained from the nerve itself. Group I underwent reconstruction with standard nerve autograft. Group II and Group III underwent reconstruction with devitalized nerve autograft treated through extracorporeal irradiation and liquid nitrogen, respectively. The left sciatic nerves of the rats served as control. Electromyography, motor function test and histomorphological analysis were performed to assess the nerve recovery on the 3rd (early stage) and 4th months (late stage). Results Electrophysiological assessment revealed better results in irradiation group compared with liquid nitrogen group in terms of myelinization and axonal regeneration. Motor performance of the autograft group was slightly better than the other groups. Histologically, autograft group demonstrated better results compared with other groups. Late-stage assessments revealed high rates of myelinization in the graft segment in liquid nitrogen group and in the segment distal to the graft in irradiation group. Conclusions This study has demonstrated that nerve autografts treated by oncological sterilization methods may be used for nerve reconstruction in limb salvage surgery. However, further studies are needed to clarify the applicability of these methods.http://dx.doi.org/10.1080/08941939.2019.1691686nerve recoverylimb salvageextracorporeal irradiationliquid nitrogennerve autograftbone tumor |
| spellingShingle | Hüseyin Kaya Dündar Sabah Burçin Keçeci Levent Küçük Oytun Erbaş Fatih Oltulu Gürkan Yiğittürk Dilek Taskiran Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model Journal of Investigative Surgery nerve recovery limb salvage extracorporeal irradiation liquid nitrogen nerve autograft bone tumor |
| title | Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model |
| title_full | Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model |
| title_fullStr | Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model |
| title_full_unstemmed | Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model |
| title_short | Comparison of the Effects of Extracorporeal Irradiation and Liquid Nitrogen on Nerve Recovery in a Rat Model |
| title_sort | comparison of the effects of extracorporeal irradiation and liquid nitrogen on nerve recovery in a rat model |
| topic | nerve recovery limb salvage extracorporeal irradiation liquid nitrogen nerve autograft bone tumor |
| url | http://dx.doi.org/10.1080/08941939.2019.1691686 |
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