Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i>
Bacterial infections caused by antibiotic-resistant pathogens are a significant public health problem. This is because the transmission of infectious diseases is shifting, and new antibiotic-resistant strains of bacteria are emerging. The development of biofilms that are resistant to antibiotics pos...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/10/484 |
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| author | Mohammed H. Alqarni Ahmed I. Foudah Aftab Alam Mohammad A. Salkini Magdy M. Muharram Nikolaos E. Labrou Pinki Rawat |
| author_facet | Mohammed H. Alqarni Ahmed I. Foudah Aftab Alam Mohammad A. Salkini Magdy M. Muharram Nikolaos E. Labrou Pinki Rawat |
| author_sort | Mohammed H. Alqarni |
| collection | DOAJ |
| description | Bacterial infections caused by antibiotic-resistant pathogens are a significant public health problem. This is because the transmission of infectious diseases is shifting, and new antibiotic-resistant strains of bacteria are emerging. The development of biofilms that are resistant to antibiotics poses another hurdle to drugs and treatment alternatives. Therefore, there is an urgent need to develop innovative strategies to effectively eliminate antibiotic-resistant microorganisms effectively. Natural coumarins have broad spectrum bioactivity and the potential for lower resistance. Coumarin is a secondary metabolite found in certain plants, fungi, and bacteria. It is highly effective against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Therefore, coumarin can be used as an alternative to combat MRSA. However, most antibacterial agents lack selective targeting of pathological sites, limiting the efficacy of their antibacterial activity. Efficient MRSA treatments can be achieved through nanoparticle (NPs)-based targeted therapies. To address this challenge, a novel coumarin-loaded solid lipid nanocarrier for MRSA was developed to overcome this challenge. The developed systems exhibited a particle size of 138.5 ± 76.06 nm and a polydispersity index (PDI) of 0.245 ± 0.00. The zeta potential of coumarin-loaded SLNs was reported to be −22.2 ± 8.15 mV with a spherical shape. The encapsulation efficiency of coumarin was reported to be 63.09 ± 3.46% in the final formulation. The developed formulation was biocompatible with a minimum inhibitory concentration (MIC) of 1.08 µg/mL. This study suggests that coumarin-loaded SLNs can effectively treat MRSA infections. |
| first_indexed | 2024-03-09T20:41:39Z |
| format | Article |
| id | doaj.art-31e633c7a5414548815f0b7674c7b835 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T20:41:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-31e633c7a5414548815f0b7674c7b8352023-11-23T22:56:44ZengMDPI AGBioengineering2306-53542022-09-0191048410.3390/bioengineering9100484Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i>Mohammed H. Alqarni0Ahmed I. Foudah1Aftab Alam2Mohammad A. Salkini3Magdy M. Muharram4Nikolaos E. Labrou5Pinki Rawat6Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi ArabiaDepartment of Microbiology, College of Science, Al-Azhar University, Nasr City 11884, Cairo, EgyptLaboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, 75 Iera Odos Street, GR-11855 Athens, GreeceMaharana Pratap College of Pharmacy, Abdul Kalam Technical University, Kanpur 209217, Uttar Pradesh, IndiaBacterial infections caused by antibiotic-resistant pathogens are a significant public health problem. This is because the transmission of infectious diseases is shifting, and new antibiotic-resistant strains of bacteria are emerging. The development of biofilms that are resistant to antibiotics poses another hurdle to drugs and treatment alternatives. Therefore, there is an urgent need to develop innovative strategies to effectively eliminate antibiotic-resistant microorganisms effectively. Natural coumarins have broad spectrum bioactivity and the potential for lower resistance. Coumarin is a secondary metabolite found in certain plants, fungi, and bacteria. It is highly effective against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). Therefore, coumarin can be used as an alternative to combat MRSA. However, most antibacterial agents lack selective targeting of pathological sites, limiting the efficacy of their antibacterial activity. Efficient MRSA treatments can be achieved through nanoparticle (NPs)-based targeted therapies. To address this challenge, a novel coumarin-loaded solid lipid nanocarrier for MRSA was developed to overcome this challenge. The developed systems exhibited a particle size of 138.5 ± 76.06 nm and a polydispersity index (PDI) of 0.245 ± 0.00. The zeta potential of coumarin-loaded SLNs was reported to be −22.2 ± 8.15 mV with a spherical shape. The encapsulation efficiency of coumarin was reported to be 63.09 ± 3.46% in the final formulation. The developed formulation was biocompatible with a minimum inhibitory concentration (MIC) of 1.08 µg/mL. This study suggests that coumarin-loaded SLNs can effectively treat MRSA infections.https://www.mdpi.com/2306-5354/9/10/484bacterial infections<i>Staphylococcus aureus</i>methicillin-resistant <i>Staphylococcus aureus</i>coumarinsolid lipid nanoparticles |
| spellingShingle | Mohammed H. Alqarni Ahmed I. Foudah Aftab Alam Mohammad A. Salkini Magdy M. Muharram Nikolaos E. Labrou Pinki Rawat Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> Bioengineering bacterial infections <i>Staphylococcus aureus</i> methicillin-resistant <i>Staphylococcus aureus</i> coumarin solid lipid nanoparticles |
| title | Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full | Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_fullStr | Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_short | Coumarin-Encapsulated Solid Lipid Nanoparticles as an Effective Therapy against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_sort | coumarin encapsulated solid lipid nanoparticles as an effective therapy against methicillin resistant i staphylococcus aureus i |
| topic | bacterial infections <i>Staphylococcus aureus</i> methicillin-resistant <i>Staphylococcus aureus</i> coumarin solid lipid nanoparticles |
| url | https://www.mdpi.com/2306-5354/9/10/484 |
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