Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament
Introduction/Objective. The aim of this study was to examine the immunohistochemical features of the vascularization of the anterior cruciate ligament (ACL), as well as the quantification of capillaries within the three segments of the ACL; proximal, middle and distal. The quantification and metric...
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| Format: | Article |
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Serbian Medical Society
2022-01-01
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| Series: | Srpski Arhiv za Celokupno Lekarstvo |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2022/0370-81792200091L.pdf |
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| author | Laketić Darko Simić Marko Boljanović Jelena Jović Darko Alexopoulos Christos G. Vitošević Biljana Zelenović Aleksandra Dinić Ljubomir Milisavljević Мilan |
| author_facet | Laketić Darko Simić Marko Boljanović Jelena Jović Darko Alexopoulos Christos G. Vitošević Biljana Zelenović Aleksandra Dinić Ljubomir Milisavljević Мilan |
| author_sort | Laketić Darko |
| collection | DOAJ |
| description | Introduction/Objective. The aim of this study was to examine the immunohistochemical features of the vascularization of the anterior cruciate ligament (ACL), as well as the quantification of capillaries within the three segments of the ACL; proximal, middle and distal. The quantification and metric characteristics of mast cells of the ACL are the second goal of this research. Methods. In total, 30 human ACL of 30 persons, obtained during routine autopsy, were examined under the microscope, following immunohistochemical reactions against CD34 of blood vessels and MastTrip of mast cells. Results. The middle genicular artery close to the ACL gave off branches for the supply of ligament itself. Each field of mm2 contained an average number of 1113.84 (959–1240), microvessels in ACL proximal third, an average number of 1145.43 (924–1310) microvessels in ACL middle third, and an average number of 1134.55 (889–1451) microvessels in ACL distal third. An average number of mast cells of the ACL was 3.8 per mm2. In the peripheral synovial zone of the ACL, we counted 12.6 mast cells per mm2. An average area value of the mast cells was 124.7 μm2, and an average value of shorter and longer axis of the mast cells was 11.2 × 15.0 μm. Conclusion. There was no statistically significant differences between the average numbers of intraligamentous microvessels of the ACL thirds (p > 0.05), confirming and supporting our hypothesis of uniform distribution of blood supply within the ACL. |
| first_indexed | 2024-04-11T06:16:10Z |
| format | Article |
| id | doaj.art-31e65d4431284cbbaf3d4898fd55f5fb |
| institution | Directory Open Access Journal |
| issn | 0370-8179 2406-0895 |
| language | English |
| last_indexed | 2024-04-11T06:16:10Z |
| publishDate | 2022-01-01 |
| publisher | Serbian Medical Society |
| record_format | Article |
| series | Srpski Arhiv za Celokupno Lekarstvo |
| spelling | doaj.art-31e65d4431284cbbaf3d4898fd55f5fb2022-12-22T04:41:02ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792406-08952022-01-011509-1057558010.2298/SARH220414091L0370-81792200091LMicroanatomical characteristics of arterial vascularization of the anterior cruciate ligamentLaketić Darko0Simić Marko1Boljanović Jelena2Jović Darko3Alexopoulos Christos G.4Vitošević Biljana5https://orcid.org/0000-0003-1234-1358Zelenović Aleksandra6Dinić Ljubomir7Milisavljević Мilan8University of Belgrade, Faculty of Medicine, Niko Miljanić Institute of Anatomy, Laboratory for Vascular Anatomy, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, University Clinical Center of Serbia, Clinic for Orthopedic Surgery and Traumatology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Niko Miljanić Institute of Anatomy, Laboratory for Vascular Anatomy, Belgrade, SerbiaUniversity Clinical Centre of Republic of Srpska, Clinic of Plastic and Reconstructive Surgery, Banja Luka, Republic of Srpska, Bosnia and HerzegovinaAcademy of Applied Preschool Teaching and Health Studies, Kruševac, Ćuprija, SerbiaUniversity of Priština, Faculty for Sports and Physical Education in Leposavić, Kosovska Mitrovica, SerbiaUniversity of Belgrade, Faculty of Medicine, Niko Miljanić Institute of Anatomy, Laboratory for Vascular Anatomy, Belgrade, SerbiaUniversity of Niš, Niš University Clinical Center, Clinic of Urology, Faculty of Medicine, Niš, SerbiaUniversity of Belgrade, Faculty of Medicine, Niko Miljanić Institute of Anatomy, Laboratory for Vascular Anatomy, Belgrade, SerbiaIntroduction/Objective. The aim of this study was to examine the immunohistochemical features of the vascularization of the anterior cruciate ligament (ACL), as well as the quantification of capillaries within the three segments of the ACL; proximal, middle and distal. The quantification and metric characteristics of mast cells of the ACL are the second goal of this research. Methods. In total, 30 human ACL of 30 persons, obtained during routine autopsy, were examined under the microscope, following immunohistochemical reactions against CD34 of blood vessels and MastTrip of mast cells. Results. The middle genicular artery close to the ACL gave off branches for the supply of ligament itself. Each field of mm2 contained an average number of 1113.84 (959–1240), microvessels in ACL proximal third, an average number of 1145.43 (924–1310) microvessels in ACL middle third, and an average number of 1134.55 (889–1451) microvessels in ACL distal third. An average number of mast cells of the ACL was 3.8 per mm2. In the peripheral synovial zone of the ACL, we counted 12.6 mast cells per mm2. An average area value of the mast cells was 124.7 μm2, and an average value of shorter and longer axis of the mast cells was 11.2 × 15.0 μm. Conclusion. There was no statistically significant differences between the average numbers of intraligamentous microvessels of the ACL thirds (p > 0.05), confirming and supporting our hypothesis of uniform distribution of blood supply within the ACL.http://www.doiserbia.nb.rs/img/doi/0370-8179/2022/0370-81792200091L.pdfanterior cruciate ligamentintraligamentous microvesselsmast cellsimmunohistochemistry |
| spellingShingle | Laketić Darko Simić Marko Boljanović Jelena Jović Darko Alexopoulos Christos G. Vitošević Biljana Zelenović Aleksandra Dinić Ljubomir Milisavljević Мilan Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament Srpski Arhiv za Celokupno Lekarstvo anterior cruciate ligament intraligamentous microvessels mast cells immunohistochemistry |
| title | Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| title_full | Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| title_fullStr | Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| title_full_unstemmed | Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| title_short | Microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| title_sort | microanatomical characteristics of arterial vascularization of the anterior cruciate ligament |
| topic | anterior cruciate ligament intraligamentous microvessels mast cells immunohistochemistry |
| url | http://www.doiserbia.nb.rs/img/doi/0370-8179/2022/0370-81792200091L.pdf |
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