The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication
In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl o...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AOSIS
2006-09-01
|
| Series: | Onderstepoort Journal of Veterinary Research |
| Online Access: | https://ojvr.org/index.php/ojvr/article/view/161 |
| _version_ | 1811229340717809664 |
|---|---|
| author | S.J. Milne A. Avenant-Oldewage |
| author_facet | S.J. Milne A. Avenant-Oldewage |
| author_sort | S.J. Milne |
| collection | DOAJ |
| description | In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms. |
| first_indexed | 2024-04-12T10:13:19Z |
| format | Article |
| id | doaj.art-31e6bdd18bd1463d9ade5eb76c8422f1 |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-04-12T10:13:19Z |
| publishDate | 2006-09-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-31e6bdd18bd1463d9ade5eb76c8422f12022-12-22T03:37:15ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352006-09-0173214915210.4102/ojvr.v73i2.161153The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communicationS.J. MilneA. Avenant-OldewageIn the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.https://ojvr.org/index.php/ojvr/article/view/161 |
| spellingShingle | S.J. Milne A. Avenant-Oldewage The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication Onderstepoort Journal of Veterinary Research |
| title | The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication |
| title_full | The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication |
| title_fullStr | The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication |
| title_full_unstemmed | The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication |
| title_short | The fluorescent detection of <i>Paradiplozoon</i> sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication |
| title_sort | fluorescent detection of i paradiplozoon i sp monogenea diplozoidae attachment clamps sclerites and integumental proteins research communication |
| url | https://ojvr.org/index.php/ojvr/article/view/161 |
| work_keys_str_mv | AT sjmilne thefluorescentdetectionofiparadiplozoonispmonogeneadiplozoidaeattachmentclampsscleritesandintegumentalproteinsresearchcommunication AT aavenantoldewage thefluorescentdetectionofiparadiplozoonispmonogeneadiplozoidaeattachmentclampsscleritesandintegumentalproteinsresearchcommunication AT sjmilne fluorescentdetectionofiparadiplozoonispmonogeneadiplozoidaeattachmentclampsscleritesandintegumentalproteinsresearchcommunication AT aavenantoldewage fluorescentdetectionofiparadiplozoonispmonogeneadiplozoidaeattachmentclampsscleritesandintegumentalproteinsresearchcommunication |