The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-drie...
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MDPI AG
2021-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/11/779 |
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| author | Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban |
| author_facet | Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban |
| author_sort | Gloria I. Solano-Aguilar |
| collection | DOAJ |
| description | A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of <i>Cryomorphaceae</i>, <i>Lachnospiraceae</i>, <i>Flammeovirgaceae</i> and <i>Ruminococcaceae</i> in the microbiome suggesting that WBM can also positively impact tissue metabolite composition. |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T05:16:31Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-31e71236449d420b9bce49c56830979b2023-11-23T00:21:25ZengMDPI AGMetabolites2218-19892021-11-01111177910.3390/metabo11110779The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic AnalysisGloria I. Solano-Aguilar0Sukla Lakshman1Saebyeol Jang2Richi Gupta3Aleksey Molokin4Steven G. Schroeder5Patrick M. Gillevet6Joseph F. Urban7Diet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAMicrobiome Analysis Center, George Mason University, Science & Technology Campus, Manassas, VA 20108, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAAnimal Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAMicrobiome Analysis Center, George Mason University, Science & Technology Campus, Manassas, VA 20108, USADiet Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture Northeast Area, Beltsville, MD 20705, USAA targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of <i>Cryomorphaceae</i>, <i>Lachnospiraceae</i>, <i>Flammeovirgaceae</i> and <i>Ruminococcaceae</i> in the microbiome suggesting that WBM can also positively impact tissue metabolite composition.https://www.mdpi.com/2218-1989/11/11/779white button mushroomtargeted metabolomicshippocampuscortexliverglycerophospholipids |
| spellingShingle | Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis Metabolites white button mushroom targeted metabolomics hippocampus cortex liver glycerophospholipids |
| title | The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis |
| title_full | The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis |
| title_fullStr | The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis |
| title_full_unstemmed | The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis |
| title_short | The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis |
| title_sort | effects of consuming white button mushroom i agaricus bisporus i on the brain and liver metabolome using a targeted metabolomic analysis |
| topic | white button mushroom targeted metabolomics hippocampus cortex liver glycerophospholipids |
| url | https://www.mdpi.com/2218-1989/11/11/779 |
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