Human Serum for Culture of Articular Chondrocytes
In the field of cell and tissue engineering, culture expansion of human cells in monolayer plays an important part. Traditionally, cell cultures have been supplemented with serum to support attachment and proliferation, but serum is a potential source of foreign protein contamination and viral prote...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-08-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000005783982909 |
| _version_ | 1819031507305496576 |
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| author | Tommi Tallheden B. Sc. Josefine Van Der Lee Camilla Brantsing Jan-Eric Månsson Eva Sjögren-Jansson Anders Lindahl |
| author_facet | Tommi Tallheden B. Sc. Josefine Van Der Lee Camilla Brantsing Jan-Eric Månsson Eva Sjögren-Jansson Anders Lindahl |
| author_sort | Tommi Tallheden B. Sc. |
| collection | DOAJ |
| description | In the field of cell and tissue engineering, culture expansion of human cells in monolayer plays an important part. Traditionally, cell cultures have been supplemented with serum to support attachment and proliferation, but serum is a potential source of foreign protein contamination and viral protein transmission. In this study, we evaluated the use of human serum for experimental human articular chondrocyte expansion and to develop a method for preparation of large volumes of high-quality human serum from healthy blood donors. Human autologous serum contained high levels of epidermal-derived growth factor and platelet-derived growth factor-AB and supported proliferation up to 7 times higher than FCS in primary chondrocyte cultures. By letting the coagulation take place in a commercially available transfusion bag overnight, up to 250 ml of growth factor-rich human serum could be obtained from one donor. The allogenic human serum supported high proliferation rate without loosing expression of cartilage-specific genes. The expanded chondrocytes were able to redifferentiate and form cartilage matrix in comparable amounts to autologous serums. In conclusion, the transfusion bags allow preparation of large volumes of growth factor-rich human serum with the capacity to support in vitro cell expansion. The data further indicate that by controlling the coagulation process there are possibilities of optimizing the release of growth factors for other emerging cell therapies. |
| first_indexed | 2024-12-21T06:47:09Z |
| format | Article |
| id | doaj.art-31ed9c72fc0049b18f67345215e53f47 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-21T06:47:09Z |
| publishDate | 2005-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-31ed9c72fc0049b18f67345215e53f472022-12-21T19:12:35ZengSAGE PublishingCell Transplantation0963-68971555-38922005-08-011410.3727/000000005783982909Human Serum for Culture of Articular ChondrocytesTommi Tallheden B. Sc.0Josefine Van Der Lee1Camilla Brantsing2Jan-Eric Månsson3Eva Sjögren-Jansson4Anders Lindahl5Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Clinical Neuroscience, Section of Experimental Neuroscience, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, SwedenDepartment of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, SwedenIn the field of cell and tissue engineering, culture expansion of human cells in monolayer plays an important part. Traditionally, cell cultures have been supplemented with serum to support attachment and proliferation, but serum is a potential source of foreign protein contamination and viral protein transmission. In this study, we evaluated the use of human serum for experimental human articular chondrocyte expansion and to develop a method for preparation of large volumes of high-quality human serum from healthy blood donors. Human autologous serum contained high levels of epidermal-derived growth factor and platelet-derived growth factor-AB and supported proliferation up to 7 times higher than FCS in primary chondrocyte cultures. By letting the coagulation take place in a commercially available transfusion bag overnight, up to 250 ml of growth factor-rich human serum could be obtained from one donor. The allogenic human serum supported high proliferation rate without loosing expression of cartilage-specific genes. The expanded chondrocytes were able to redifferentiate and form cartilage matrix in comparable amounts to autologous serums. In conclusion, the transfusion bags allow preparation of large volumes of growth factor-rich human serum with the capacity to support in vitro cell expansion. The data further indicate that by controlling the coagulation process there are possibilities of optimizing the release of growth factors for other emerging cell therapies.https://doi.org/10.3727/000000005783982909 |
| spellingShingle | Tommi Tallheden B. Sc. Josefine Van Der Lee Camilla Brantsing Jan-Eric Månsson Eva Sjögren-Jansson Anders Lindahl Human Serum for Culture of Articular Chondrocytes Cell Transplantation |
| title | Human Serum for Culture of Articular Chondrocytes |
| title_full | Human Serum for Culture of Articular Chondrocytes |
| title_fullStr | Human Serum for Culture of Articular Chondrocytes |
| title_full_unstemmed | Human Serum for Culture of Articular Chondrocytes |
| title_short | Human Serum for Culture of Articular Chondrocytes |
| title_sort | human serum for culture of articular chondrocytes |
| url | https://doi.org/10.3727/000000005783982909 |
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