Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion
Background and aims: Renal ischemia-reperfusion occurs in some clinical conditions such as kidney surgery that can leads to acute renal failure. The aim of this study was to investigate the effect of p-coumaric acid (CA) on ischemia reperfusion (I/R) injury. Methods: Thirty rats were randomly divide...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Spanish |
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Elsevier
2020-05-01
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| Series: | Nefrología |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0211699519301936 |
| _version_ | 1818041480623685632 |
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| author | Shahin Mozaffari Godarzi Armita Valizade Gorji Behnam Gholizadeh Seyyed Ali Mard Esrafil Mansouri |
| author_facet | Shahin Mozaffari Godarzi Armita Valizade Gorji Behnam Gholizadeh Seyyed Ali Mard Esrafil Mansouri |
| author_sort | Shahin Mozaffari Godarzi |
| collection | DOAJ |
| description | Background and aims: Renal ischemia-reperfusion occurs in some clinical conditions such as kidney surgery that can leads to acute renal failure. The aim of this study was to investigate the effect of p-coumaric acid (CA) on ischemia reperfusion (I/R) injury. Methods: Thirty rats were randomly divided into five groups; control, CA (100 mg/kg), I/R, propylene glycol (10%) + I/R and CA + I/R, (n = 6 each). CA and propylene glycol were administered orally for 2 weeks. Then, the rats were subjected to bilateral renal ischemia for 45 min and followed by reperfusion for 24 h. All rats were killed and kidney function tests, tissue malondialdehyde and activity of antioxidant enzymes were determined. Histopathological evaluations were also performed. In addition, renal expression of the tumor necrosis factor-α and interleukin-1β were determined using enzyme-linked immunosorbent assay and immunohistochemistry. Results: CA significantly improved the Cr and BUN levels in CA + I/R group compared to I/R group (p < 0.005 and p < 0.001, respectively). Reduction of tissue superoxide dismutase, glutathione peroxidase and catalase, were significantly improved by CA (p < 0.01, p < 0.01 and p < 0.05). Treatment with CA also resulted in significant reduction in tissue MDA (p < 0.05), TNF-α (p < 0.001) and interleukin-1β expression (p < 0.001) that were increased by renal I/R. Also, the rats treated with CA had nearly normal structure of the kidney. Conclusions: The present findings suggest that, CA protects the kidneys against I/R injury via its antioxidant and anti-inflammatory effects. Resumen: Antecedentes y objetivos: La isquemia-reperfusión renal se produce en algunas situaciones clínicas como la cirugía renal, y puede conducir a insuficiencia renal aguda. El objetivo de este estudio fue investigar el efecto del ácido p-cumárico (AC) en el daño por isquemia-reperfusión (I/R). Métodos: Se dividió aleatoriamente a 30 ratas en 5 grupos; control, AC (100 mg/kg), I/R, propilenglicol, (10%) + I/R y AC + I/R, (n = 6 cada uno). El AC y el propilenglicol se administraron por vía oral durante 2 semanas. A continuación, las ratas se sometieron a isquemia renal bilateral durante 45 min, seguido de reperfusión durante 24 h. Se sacrificó a todas las ratas y se determinaron los valores de la función renal, el malondialdehído tisular y la actividad de las enzimas antioxidantes. También se llevaron a cabo evaluaciones histopatológicas. Además, se determinó la expresión renal del factor de necrosis tumoral-α y la interleucina-1β mediante enzimoinmunoanálisis de adsorción e inmunohistoquímica. Resultados: El AC mejoró significativamente los niveles de Cr y BUN en el grupo de AC + I/R en comparación con el grupo de I/R (p < 0,005 y p < 0,001, respectivamente). La reducción de la superóxido-dismutasa tisular, la glutatión-peroxidasa y la catalasa mejoró significativamente con el AC (p < 0,01, p < 0,01 y p < 0,05, respectivamente). El tratamiento con AC también provocó una reducción significativa de la expresión del malondialdehído (MDA) tisular (p < 0,05), el TNF-α (p < 0,001) y la interleucina-1β (p < 0,001) que habían aumentada por la I/R renal. Además, las ratas tratadas con AC presentaron una estructura renal casi normal. Conclusiones: Estos hallazgos sugieren que el AC protege los riñones frente al daño por I/R a través de sus efectos antioxidantes y antinflamatorios. |
| first_indexed | 2024-12-10T08:31:06Z |
| format | Article |
| id | doaj.art-31f19b3fd02649bcaddf6c77f750b9ee |
| institution | Directory Open Access Journal |
| issn | 0211-6995 |
| language | Spanish |
| last_indexed | 2024-12-10T08:31:06Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Nefrología |
| spelling | doaj.art-31f19b3fd02649bcaddf6c77f750b9ee2022-12-22T01:56:05ZspaElsevierNefrología0211-69952020-05-01403311319Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusionShahin Mozaffari Godarzi0Armita Valizade Gorji1Behnam Gholizadeh2Seyyed Ali Mard3Esrafil Mansouri4Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Cardiac Surgery, Atherosclerosis Research Center and Imam Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranPhysiology Research Center (PRC), Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Corresponding author.Background and aims: Renal ischemia-reperfusion occurs in some clinical conditions such as kidney surgery that can leads to acute renal failure. The aim of this study was to investigate the effect of p-coumaric acid (CA) on ischemia reperfusion (I/R) injury. Methods: Thirty rats were randomly divided into five groups; control, CA (100 mg/kg), I/R, propylene glycol (10%) + I/R and CA + I/R, (n = 6 each). CA and propylene glycol were administered orally for 2 weeks. Then, the rats were subjected to bilateral renal ischemia for 45 min and followed by reperfusion for 24 h. All rats were killed and kidney function tests, tissue malondialdehyde and activity of antioxidant enzymes were determined. Histopathological evaluations were also performed. In addition, renal expression of the tumor necrosis factor-α and interleukin-1β were determined using enzyme-linked immunosorbent assay and immunohistochemistry. Results: CA significantly improved the Cr and BUN levels in CA + I/R group compared to I/R group (p < 0.005 and p < 0.001, respectively). Reduction of tissue superoxide dismutase, glutathione peroxidase and catalase, were significantly improved by CA (p < 0.01, p < 0.01 and p < 0.05). Treatment with CA also resulted in significant reduction in tissue MDA (p < 0.05), TNF-α (p < 0.001) and interleukin-1β expression (p < 0.001) that were increased by renal I/R. Also, the rats treated with CA had nearly normal structure of the kidney. Conclusions: The present findings suggest that, CA protects the kidneys against I/R injury via its antioxidant and anti-inflammatory effects. Resumen: Antecedentes y objetivos: La isquemia-reperfusión renal se produce en algunas situaciones clínicas como la cirugía renal, y puede conducir a insuficiencia renal aguda. El objetivo de este estudio fue investigar el efecto del ácido p-cumárico (AC) en el daño por isquemia-reperfusión (I/R). Métodos: Se dividió aleatoriamente a 30 ratas en 5 grupos; control, AC (100 mg/kg), I/R, propilenglicol, (10%) + I/R y AC + I/R, (n = 6 cada uno). El AC y el propilenglicol se administraron por vía oral durante 2 semanas. A continuación, las ratas se sometieron a isquemia renal bilateral durante 45 min, seguido de reperfusión durante 24 h. Se sacrificó a todas las ratas y se determinaron los valores de la función renal, el malondialdehído tisular y la actividad de las enzimas antioxidantes. También se llevaron a cabo evaluaciones histopatológicas. Además, se determinó la expresión renal del factor de necrosis tumoral-α y la interleucina-1β mediante enzimoinmunoanálisis de adsorción e inmunohistoquímica. Resultados: El AC mejoró significativamente los niveles de Cr y BUN en el grupo de AC + I/R en comparación con el grupo de I/R (p < 0,005 y p < 0,001, respectivamente). La reducción de la superóxido-dismutasa tisular, la glutatión-peroxidasa y la catalasa mejoró significativamente con el AC (p < 0,01, p < 0,01 y p < 0,05, respectivamente). El tratamiento con AC también provocó una reducción significativa de la expresión del malondialdehído (MDA) tisular (p < 0,05), el TNF-α (p < 0,001) y la interleucina-1β (p < 0,001) que habían aumentada por la I/R renal. Además, las ratas tratadas con AC presentaron una estructura renal casi normal. Conclusiones: Estos hallazgos sugieren que el AC protege los riñones frente al daño por I/R a través de sus efectos antioxidantes y antinflamatorios.http://www.sciencedirect.com/science/article/pii/S0211699519301936Isquemia-reperfusión renalEstrés oxidativoÁcido p-cumáricoTNF-αIL-1β |
| spellingShingle | Shahin Mozaffari Godarzi Armita Valizade Gorji Behnam Gholizadeh Seyyed Ali Mard Esrafil Mansouri Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion Nefrología Isquemia-reperfusión renal Estrés oxidativo Ácido p-cumárico TNF-α IL-1β |
| title | Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion |
| title_full | Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion |
| title_fullStr | Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion |
| title_full_unstemmed | Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion |
| title_short | Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion |
| title_sort | antioxidant effect of p coumaric acid on interleukin 1 β and tumor necrosis factor α in rats with renal ischemic reperfusion |
| topic | Isquemia-reperfusión renal Estrés oxidativo Ácido p-cumárico TNF-α IL-1β |
| url | http://www.sciencedirect.com/science/article/pii/S0211699519301936 |
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