Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings
IntroductionThe first peak of COVID-19 in Brazil was between April and May 2020, at a time of the year when outbreaks of other tropical diseases, such as dengue, would be expected. COVID-19 and dengue have similar pathogenesis. In general, both may lead to mild symptoms but may also cause severe and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Tropical Diseases |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fitd.2023.1249574/full |
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| author | Joyce Carnevale Rodrigues Débora Familiar-Macedo Thalia Medeiros Thalia Medeiros Fabiana Rabe Carvalho Jorge Reis Almeida Andrea Alice Silva Andrea Alice Silva Flávia Barreto dos Santos Luiz José de Souza Paulo Vieira Damasco Paulo Vieira Damasco Paulo Vieira Damasco Elzinandes Leal Azeredo Luzia Maria de-Oliveira-Pinto |
| author_facet | Joyce Carnevale Rodrigues Débora Familiar-Macedo Thalia Medeiros Thalia Medeiros Fabiana Rabe Carvalho Jorge Reis Almeida Andrea Alice Silva Andrea Alice Silva Flávia Barreto dos Santos Luiz José de Souza Paulo Vieira Damasco Paulo Vieira Damasco Paulo Vieira Damasco Elzinandes Leal Azeredo Luzia Maria de-Oliveira-Pinto |
| author_sort | Joyce Carnevale Rodrigues |
| collection | DOAJ |
| description | IntroductionThe first peak of COVID-19 in Brazil was between April and May 2020, at a time of the year when outbreaks of other tropical diseases, such as dengue, would be expected. COVID-19 and dengue have similar pathogenesis. In general, both may lead to mild symptoms but may also cause severe and even fatal symptoms, especially in patients with comorbidities and probably in cases of overlapping infections. The general objective of this study was to assess whether, during the 2020 pandemic, there were cases of concomitant infection between SARS-CoV-2 and DENV.MethodsFor this, we evaluated the specificity and sensitivity of commercial serological anti-SARS-CoV-2 kits using plasma samples from patients with dengue and healthy donors recruited before COVID-19. In the case of confirmed cases of COVID-19/dengue, we evaluated the clinical evolution of these coinfected patients, compared with mono-infected patients; and quantified chemokines CCL2 and CXCL8 by ELISA in COVID-19 patients in order to correlate them with COVID-19/dengue severity and cases.Results and DiscussionOur results showed that commercial IgA and IgG anti-SARS-CoV-2 kits presented high sensitivity and specificity. This allowed us to see a low rate of co-detection or coinfection between SARS-CoV-2 and DENV in Rio de Janeiro. Among the 57 COVID-19 patients, anti-DENV IgM was detected in five (8.8%). COVID-19/dengue coinfected patients showed no clinical worsening of COVID-19 and cases in which COVID-19 patients had previous exposure to DENV did not influence the clinical severity of COVID-19. Lastly, CCL2 and CXCL8 appeared to be good markers of COVID-19 severity and did not show increased levels in COVID-19/dengue cases. |
| first_indexed | 2024-03-11T17:37:36Z |
| format | Article |
| id | doaj.art-31fba5ecca1840c5b3dcb0e7e73eff58 |
| institution | Directory Open Access Journal |
| issn | 2673-7515 |
| language | English |
| last_indexed | 2024-03-11T17:37:36Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Tropical Diseases |
| spelling | doaj.art-31fba5ecca1840c5b3dcb0e7e73eff582023-10-18T18:04:38ZengFrontiers Media S.A.Frontiers in Tropical Diseases2673-75152023-10-01410.3389/fitd.2023.12495741249574Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findingsJoyce Carnevale Rodrigues0Débora Familiar-Macedo1Thalia Medeiros2Thalia Medeiros3Fabiana Rabe Carvalho4Jorge Reis Almeida5Andrea Alice Silva6Andrea Alice Silva7Flávia Barreto dos Santos8Luiz José de Souza9Paulo Vieira Damasco10Paulo Vieira Damasco11Paulo Vieira Damasco12Elzinandes Leal Azeredo13Luzia Maria de-Oliveira-Pinto14Laboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, BrazilLaboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, BrazilLaboratório Multiusuário de Apoio à Pesquisa em Nefrologia e Ciências Médicas, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilDepartamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilLaboratório Multiusuário de Apoio à Pesquisa em Nefrologia e Ciências Médicas, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilDepartamento de Clínica Médica, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilLaboratório Multiusuário de Apoio à Pesquisa em Nefrologia e Ciências Médicas, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilDepartamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, BrazilLaboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, BrazilHospital Plantadores de Cana, Centro de Referência de Doenças Imuno-Infecciosas, Campos dos Goytacazes, BrazilRede Casa Hospital Rio Laranjeiras e Rio Botafogo, Rio de Janeiro, BrazilEscola de Medicina e Cirurgia, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, BrazilFaculdade de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, BrazilLaboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz (IOC/Fiocruz), Rio de Janeiro, BrazilIntroductionThe first peak of COVID-19 in Brazil was between April and May 2020, at a time of the year when outbreaks of other tropical diseases, such as dengue, would be expected. COVID-19 and dengue have similar pathogenesis. In general, both may lead to mild symptoms but may also cause severe and even fatal symptoms, especially in patients with comorbidities and probably in cases of overlapping infections. The general objective of this study was to assess whether, during the 2020 pandemic, there were cases of concomitant infection between SARS-CoV-2 and DENV.MethodsFor this, we evaluated the specificity and sensitivity of commercial serological anti-SARS-CoV-2 kits using plasma samples from patients with dengue and healthy donors recruited before COVID-19. In the case of confirmed cases of COVID-19/dengue, we evaluated the clinical evolution of these coinfected patients, compared with mono-infected patients; and quantified chemokines CCL2 and CXCL8 by ELISA in COVID-19 patients in order to correlate them with COVID-19/dengue severity and cases.Results and DiscussionOur results showed that commercial IgA and IgG anti-SARS-CoV-2 kits presented high sensitivity and specificity. This allowed us to see a low rate of co-detection or coinfection between SARS-CoV-2 and DENV in Rio de Janeiro. Among the 57 COVID-19 patients, anti-DENV IgM was detected in five (8.8%). COVID-19/dengue coinfected patients showed no clinical worsening of COVID-19 and cases in which COVID-19 patients had previous exposure to DENV did not influence the clinical severity of COVID-19. Lastly, CCL2 and CXCL8 appeared to be good markers of COVID-19 severity and did not show increased levels in COVID-19/dengue cases.https://www.frontiersin.org/articles/10.3389/fitd.2023.1249574/fullCOVID-19denguecoinfectiondiagnosishuman |
| spellingShingle | Joyce Carnevale Rodrigues Débora Familiar-Macedo Thalia Medeiros Thalia Medeiros Fabiana Rabe Carvalho Jorge Reis Almeida Andrea Alice Silva Andrea Alice Silva Flávia Barreto dos Santos Luiz José de Souza Paulo Vieira Damasco Paulo Vieira Damasco Paulo Vieira Damasco Elzinandes Leal Azeredo Luzia Maria de-Oliveira-Pinto Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings Frontiers in Tropical Diseases COVID-19 dengue coinfection diagnosis human |
| title | Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings |
| title_full | Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings |
| title_fullStr | Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings |
| title_full_unstemmed | Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings |
| title_short | Assessment of threat of concurrent SARS-CoV-2 and DENV infection in the COVID-19 pandemic in Brazil in 2020: diagnostic and immunological findings |
| title_sort | assessment of threat of concurrent sars cov 2 and denv infection in the covid 19 pandemic in brazil in 2020 diagnostic and immunological findings |
| topic | COVID-19 dengue coinfection diagnosis human |
| url | https://www.frontiersin.org/articles/10.3389/fitd.2023.1249574/full |
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