Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks
Abstract Due to the reduction of the jaguar population, the formation of somatic cell cryobanks represents an interesting tool for its conservation. Nevertheless, the success of these cryobanks depends on the cryoprotectants used in cryopreservation. We evaluated the effects of the intracellular cry...
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| Format: | Article |
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Academia Brasileira de Ciências
2021-10-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000701307&tlng=en |
| _version_ | 1798014957846003712 |
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| author | LHARA R.M. DE OLIVEIRA ÉRIKA A. PRAXEDES MARIA B. SILVA LEANDRO R. RIBEIRO HERLON V.R. SILVA ALEXSANDRA F. PEREIRA |
| author_facet | LHARA R.M. DE OLIVEIRA ÉRIKA A. PRAXEDES MARIA B. SILVA LEANDRO R. RIBEIRO HERLON V.R. SILVA ALEXSANDRA F. PEREIRA |
| author_sort | LHARA R.M. DE OLIVEIRA |
| collection | DOAJ |
| description | Abstract Due to the reduction of the jaguar population, the formation of somatic cell cryobanks represents an interesting tool for its conservation. Nevertheless, the success of these cryobanks depends on the cryoprotectants used in cryopreservation. We evaluated the effects of the intracellular cryoprotectants (10% dimethyl sulfoxide, DMSO; 10% ethylene glycol, EG) in the absence or presence of an extracellular cryoprotectant (0.2 M sucrose, SUC) on the morphology, confluence, viability, and metabolism of somatic cells derived from five jaguars belonging to Brazilian zoos. The morphology was presented in a descriptive manner, while the confluence, viability and metabolic activity were presented as means and compared using statistical tests. Non-cryopreserved cells were used as control and compared to frozen/thawed cells using cryoprotectants. No difference was observed for the morphology and confluence among non-cryopreserved and cryopreserved cells, regardless of the cryoprotectants. Only cryopreserved cells in EG (45.8%±12.9) had a reduction in their viability when compared to non-cryopreserved cells (97.8%±1.1). Only cryopreserved cells in DMSO with SUC (76.0%±2.7) or absence of SUC (77.0%±3.7) maintained their metabolic activity after thawing, when compared to non-cryopreserved cells (100.0%±6.7). Therefore, combinations of DMSO in the absence and presence of SUC were efficient in the cryopreservation of somatic cells of jaguars. |
| first_indexed | 2024-04-11T15:26:34Z |
| format | Article |
| id | doaj.art-31fbe975bd35433bba3afe67aec33fc7 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T15:26:34Z |
| publishDate | 2021-10-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-31fbe975bd35433bba3afe67aec33fc72022-12-22T04:16:14ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-10-0193410.1590/0001-3765202120190314Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banksLHARA R.M. DE OLIVEIRAhttps://orcid.org/0000-0002-7292-6908ÉRIKA A. PRAXEDEShttps://orcid.org/0000-0002-6406-6346MARIA B. SILVAhttps://orcid.org/0000-0001-5745-3279LEANDRO R. RIBEIROhttps://orcid.org/0000-0001-7788-8211HERLON V.R. SILVAhttps://orcid.org/0000-0003-1327-9613ALEXSANDRA F. PEREIRAhttps://orcid.org/0000-0003-2137-854XAbstract Due to the reduction of the jaguar population, the formation of somatic cell cryobanks represents an interesting tool for its conservation. Nevertheless, the success of these cryobanks depends on the cryoprotectants used in cryopreservation. We evaluated the effects of the intracellular cryoprotectants (10% dimethyl sulfoxide, DMSO; 10% ethylene glycol, EG) in the absence or presence of an extracellular cryoprotectant (0.2 M sucrose, SUC) on the morphology, confluence, viability, and metabolism of somatic cells derived from five jaguars belonging to Brazilian zoos. The morphology was presented in a descriptive manner, while the confluence, viability and metabolic activity were presented as means and compared using statistical tests. Non-cryopreserved cells were used as control and compared to frozen/thawed cells using cryoprotectants. No difference was observed for the morphology and confluence among non-cryopreserved and cryopreserved cells, regardless of the cryoprotectants. Only cryopreserved cells in EG (45.8%±12.9) had a reduction in their viability when compared to non-cryopreserved cells (97.8%±1.1). Only cryopreserved cells in DMSO with SUC (76.0%±2.7) or absence of SUC (77.0%±3.7) maintained their metabolic activity after thawing, when compared to non-cryopreserved cells (100.0%±6.7). Therefore, combinations of DMSO in the absence and presence of SUC were efficient in the cryopreservation of somatic cells of jaguars.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000701307&tlng=enBiological resource bankscryopreservationPanthera genusslow freezingwild felids |
| spellingShingle | LHARA R.M. DE OLIVEIRA ÉRIKA A. PRAXEDES MARIA B. SILVA LEANDRO R. RIBEIRO HERLON V.R. SILVA ALEXSANDRA F. PEREIRA Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks Anais da Academia Brasileira de Ciências Biological resource banks cryopreservation Panthera genus slow freezing wild felids |
| title | Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks |
| title_full | Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks |
| title_fullStr | Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks |
| title_full_unstemmed | Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks |
| title_short | Comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar, Panthera onca, towards the formation of biologic banks |
| title_sort | comparative effect of cryoprotectant combinations on the conservation of somatic cells derived from jaguar panthera onca towards the formation of biologic banks |
| topic | Biological resource banks cryopreservation Panthera genus slow freezing wild felids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000701307&tlng=en |
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