Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study
Background and Aim: Salmonellosis is a significant foodborne disease that causes serious illness in the gastrointestinal of humans and it is a public health problem worldwide. This study aimed to determine Salmonella spp. in animal feeds, its characteristic, serovar identification, genotyping, and d...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-05-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.16/May-2023/6.pdf |
| _version_ | 1797831533089783808 |
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| author | Arsooth Sanguankiat Nayika Pinniam Phitsanu Tulayakul |
| author_facet | Arsooth Sanguankiat Nayika Pinniam Phitsanu Tulayakul |
| author_sort | Arsooth Sanguankiat |
| collection | DOAJ |
| description | Background and Aim: Salmonellosis is a significant foodborne disease that causes serious illness in the gastrointestinal of humans and it is a public health problem worldwide. This study aimed to determine Salmonella spp. in animal feeds, its characteristic, serovar identification, genotyping, and drug sensitivity.
Materials and Methods: Salmonella spp. from animal feedstuffs was collected from January 1 to December 31, 2017, with 657 samples. Serogroup classification was performed by using the slide agglutination test. Then, the samples were analyzed for genotype patterns using pulsed-field gel electrophoresis (PFGE) for DNA fingerprint and antibiotic sensitivity by Vitek® 2 techniques.
Results: A total of 80 samples (12.17 %) were found to be Salmonella positive; commercial feed compounds of 60 samples (75%); soybean meal of 10 samples (12.5%); pork meal of 5 samples (6.25%); a fish meal of three samples (3.75%) and poultry meal of 2 samples (2.5%). Serogroups B, C, D, and E were found in this study; 8 samples (10%), 39 samples (48.75%), 8 samples (10%), and 13 samples (16.25%), respectively. A total of 12 samples (15%) were not determined by serogrouping. The most common serovars were Salmonella Rissen (10%), Salmonella Mbandaka (8.75%), and Salmonella Livingstone (6.25%), which belong to serogroup C. Nine of eleven pulsotypes were detected when analyzed by PFGE, showed similarity index between 40.8 and 100 %. Antimicrobial susceptibility tests by Vitek® 2 compact for 11 strains were classified into three groups: resistance to 4, 8, and 11 antibiotics, out of 20 antibiotics.
Conclusion: This study revealed annual variation of Salmonella spp. Serovar, genotype, and phenotype from commercial feed compounds and raw materials of which involved people must be aware. |
| first_indexed | 2024-04-09T13:54:20Z |
| format | Article |
| id | doaj.art-31fc48a625a64a36b4fae63896930ebd |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-09T13:54:20Z |
| publishDate | 2023-05-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-31fc48a625a64a36b4fae63896930ebd2023-05-08T11:06:56ZengVeterinary WorldVeterinary World0972-89882231-09162023-05-0116593994510.14202/vetworld.2023.939-945Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual studyArsooth Sanguankiat0https://orcid.org/0000-0001-7650-5142Nayika Pinniam1https://orcid.org/0000-0002-0245-1927Phitsanu Tulayakul2https://orcid.org/0000-0003-4283-1497Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.Background and Aim: Salmonellosis is a significant foodborne disease that causes serious illness in the gastrointestinal of humans and it is a public health problem worldwide. This study aimed to determine Salmonella spp. in animal feeds, its characteristic, serovar identification, genotyping, and drug sensitivity. Materials and Methods: Salmonella spp. from animal feedstuffs was collected from January 1 to December 31, 2017, with 657 samples. Serogroup classification was performed by using the slide agglutination test. Then, the samples were analyzed for genotype patterns using pulsed-field gel electrophoresis (PFGE) for DNA fingerprint and antibiotic sensitivity by Vitek® 2 techniques. Results: A total of 80 samples (12.17 %) were found to be Salmonella positive; commercial feed compounds of 60 samples (75%); soybean meal of 10 samples (12.5%); pork meal of 5 samples (6.25%); a fish meal of three samples (3.75%) and poultry meal of 2 samples (2.5%). Serogroups B, C, D, and E were found in this study; 8 samples (10%), 39 samples (48.75%), 8 samples (10%), and 13 samples (16.25%), respectively. A total of 12 samples (15%) were not determined by serogrouping. The most common serovars were Salmonella Rissen (10%), Salmonella Mbandaka (8.75%), and Salmonella Livingstone (6.25%), which belong to serogroup C. Nine of eleven pulsotypes were detected when analyzed by PFGE, showed similarity index between 40.8 and 100 %. Antimicrobial susceptibility tests by Vitek® 2 compact for 11 strains were classified into three groups: resistance to 4, 8, and 11 antibiotics, out of 20 antibiotics. Conclusion: This study revealed annual variation of Salmonella spp. Serovar, genotype, and phenotype from commercial feed compounds and raw materials of which involved people must be aware.https://www.veterinaryworld.org/Vol.16/May-2023/6.pdfcommercial feed compoundsgenotypesalmonella spp.serotype |
| spellingShingle | Arsooth Sanguankiat Nayika Pinniam Phitsanu Tulayakul Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study Veterinary World commercial feed compounds genotype salmonella spp. serotype |
| title | Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study |
| title_full | Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study |
| title_fullStr | Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study |
| title_full_unstemmed | Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study |
| title_short | Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study |
| title_sort | surveillance of antimicrobial resistance phenotypic and genotypic patterns of salmonella enterica isolated from animal feedstuffs annual study |
| topic | commercial feed compounds genotype salmonella spp. serotype |
| url | https://www.veterinaryworld.org/Vol.16/May-2023/6.pdf |
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