Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients
Gaining long-term graft function and patient life quality remain critical challenges following kidney transplantation. Advances in immunology, gnotobiotics, and culture-independent molecular techniques have provided growing insights into the complex relationship of the microbiome and the host. Howev...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/11/1724 |
| _version_ | 1797548864889159680 |
|---|---|
| author | Nessrine Souai Oumaima Zidi Amor Mosbah Imen Kosai Jameleddine El Manaa Naima Bel Mokhtar Elias Asimakis Panagiota Stathopoulou Ameur Cherif George Tsiamis Soumaya Kouidhi |
| author_facet | Nessrine Souai Oumaima Zidi Amor Mosbah Imen Kosai Jameleddine El Manaa Naima Bel Mokhtar Elias Asimakis Panagiota Stathopoulou Ameur Cherif George Tsiamis Soumaya Kouidhi |
| author_sort | Nessrine Souai |
| collection | DOAJ |
| description | Gaining long-term graft function and patient life quality remain critical challenges following kidney transplantation. Advances in immunology, gnotobiotics, and culture-independent molecular techniques have provided growing insights into the complex relationship of the microbiome and the host. However, little is known about the over time-shift of the gut microbiota in the context of kidney transplantation and its impact on both graft and health stability. Here we aimed to characterize the structure of gut microbiota within stable kidney graft recipients. We enrolled forty kidney transplant patients after at least three months of transplantation and compared them to eighteen healthy controls. The overall microbial community structure of the kidney transplanted group was clearly different from control subjects. We found lower relative abundances of Actinobacteria, Bacteroidetes, and Verrucomicrobia within the patient group and a higher abundance of Proteobacteria compared to the control group. Both richness and Shannon diversity indexes were significantly lower in the kidney graft recipients than in healthy controls. Post-graft period was positively correlated with the relative abundance of the Proteobacteria phylum, especially <i>Escherichia.Shigella</i> genus. Interestingly, only <i>Parabacteroides</i> was found to significantly differentiate patients that were not suffering from lifestyle diseases and those who suffer from post-graft complications. Furthermore, network analysis showed that the occurrence of lifestyle diseases was significantly linked with a higher number of negative interactions of <i>Sutterella</i> and <i>Succinivibrio</i> genera within patients. This study characterizes gut microbiome fluctuation in stable kidney transplant patients after a long post-allograft period. Analysis of fecal microbiota could be useful for nephrologists as a new clinical tool that can improve kidney allograft monitoring and outcomes. |
| first_indexed | 2024-03-10T15:05:53Z |
| format | Article |
| id | doaj.art-31fc911a9fb049c398b7076a6ca455b7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:05:53Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-31fc911a9fb049c398b7076a6ca455b72023-11-20T19:44:02ZengMDPI AGMicroorganisms2076-26072020-11-01811172410.3390/microorganisms8111724Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft RecipientsNessrine Souai0Oumaima Zidi1Amor Mosbah2Imen Kosai3Jameleddine El Manaa4Naima Bel Mokhtar5Elias Asimakis6Panagiota Stathopoulou7Ameur Cherif8George Tsiamis9Soumaya Kouidhi10Laboratory of Biotechnology and Valorisation of Bio-GeoRessources, Higher Institute of Biotechnology of Sidi Thabet, BiotechPole of Sidi Thabet, University of Manouba, Ariana 2020, TunisiaLaboratory of Biotechnology and Valorisation of Bio-GeoRessources, Higher Institute of Biotechnology of Sidi Thabet, BiotechPole of Sidi Thabet, University of Manouba, Ariana 2020, TunisiaLaboratory of Biotechnology and Valorisation of Bio-GeoRessources, Higher Institute of Biotechnology of Sidi Thabet, BiotechPole of Sidi Thabet, University of Manouba, Ariana 2020, TunisiaUnit of Organ Transplant Military Training Hospital, Mont Fleury 1008, Tunis, TunisiaUnit of Organ Transplant Military Training Hospital, Mont Fleury 1008, Tunis, TunisiaLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceLaboratory of Biotechnology and Valorisation of Bio-GeoRessources, Higher Institute of Biotechnology of Sidi Thabet, BiotechPole of Sidi Thabet, University of Manouba, Ariana 2020, TunisiaLaboratory of Systems Microbiology and Applied Genomics, Department of Environmental Engineering, University of Patras, 2 Seferi St, 30100 Agrinio, GreeceLaboratory of Biotechnology and Valorisation of Bio-GeoRessources, Higher Institute of Biotechnology of Sidi Thabet, BiotechPole of Sidi Thabet, University of Manouba, Ariana 2020, TunisiaGaining long-term graft function and patient life quality remain critical challenges following kidney transplantation. Advances in immunology, gnotobiotics, and culture-independent molecular techniques have provided growing insights into the complex relationship of the microbiome and the host. However, little is known about the over time-shift of the gut microbiota in the context of kidney transplantation and its impact on both graft and health stability. Here we aimed to characterize the structure of gut microbiota within stable kidney graft recipients. We enrolled forty kidney transplant patients after at least three months of transplantation and compared them to eighteen healthy controls. The overall microbial community structure of the kidney transplanted group was clearly different from control subjects. We found lower relative abundances of Actinobacteria, Bacteroidetes, and Verrucomicrobia within the patient group and a higher abundance of Proteobacteria compared to the control group. Both richness and Shannon diversity indexes were significantly lower in the kidney graft recipients than in healthy controls. Post-graft period was positively correlated with the relative abundance of the Proteobacteria phylum, especially <i>Escherichia.Shigella</i> genus. Interestingly, only <i>Parabacteroides</i> was found to significantly differentiate patients that were not suffering from lifestyle diseases and those who suffer from post-graft complications. Furthermore, network analysis showed that the occurrence of lifestyle diseases was significantly linked with a higher number of negative interactions of <i>Sutterella</i> and <i>Succinivibrio</i> genera within patients. This study characterizes gut microbiome fluctuation in stable kidney transplant patients after a long post-allograft period. Analysis of fecal microbiota could be useful for nephrologists as a new clinical tool that can improve kidney allograft monitoring and outcomes.https://www.mdpi.com/2076-2607/8/11/172416S rRNA geneamplicon sequencingdysbiosisbioinformaticskidney transplantation |
| spellingShingle | Nessrine Souai Oumaima Zidi Amor Mosbah Imen Kosai Jameleddine El Manaa Naima Bel Mokhtar Elias Asimakis Panagiota Stathopoulou Ameur Cherif George Tsiamis Soumaya Kouidhi Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients Microorganisms 16S rRNA gene amplicon sequencing dysbiosis bioinformatics kidney transplantation |
| title | Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients |
| title_full | Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients |
| title_fullStr | Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients |
| title_full_unstemmed | Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients |
| title_short | Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients |
| title_sort | impact of the post transplant period and lifestyle diseases on human gut microbiota in kidney graft recipients |
| topic | 16S rRNA gene amplicon sequencing dysbiosis bioinformatics kidney transplantation |
| url | https://www.mdpi.com/2076-2607/8/11/1724 |
| work_keys_str_mv | AT nessrinesouai impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT oumaimazidi impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT amormosbah impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT imenkosai impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT jameleddineelmanaa impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT naimabelmokhtar impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT eliasasimakis impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT panagiotastathopoulou impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT ameurcherif impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT georgetsiamis impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients AT soumayakouidhi impactoftheposttransplantperiodandlifestylediseasesonhumangutmicrobiotainkidneygraftrecipients |