Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications
A sample preconcentration technique that exceeds a microfluidic device’s limited processing volume (up to microliters) is critical for real sample pretreatment applications. Here, we have developed a 3D-printed preconcentrator with a pillar structure (3DP2) to enrich the biological samples up to hun...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Sensors |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsens.2022.1066974/full |
| _version_ | 1811189127759003648 |
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| author | Seungmin Lee Seungmin Lee Jinhwan Kim Na Eun Lee Na Eun Lee Kang Hyeon Kim Seong Jun Park Jeong Soo Park Cheonjung Kim Cheonjung Kim Ji Hye Hong Ji Hye Hong Dae Sung Yoon Yong Kyoung Yoo Jeong Hoon Lee |
| author_facet | Seungmin Lee Seungmin Lee Jinhwan Kim Na Eun Lee Na Eun Lee Kang Hyeon Kim Seong Jun Park Jeong Soo Park Cheonjung Kim Cheonjung Kim Ji Hye Hong Ji Hye Hong Dae Sung Yoon Yong Kyoung Yoo Jeong Hoon Lee |
| author_sort | Seungmin Lee |
| collection | DOAJ |
| description | A sample preconcentration technique that exceeds a microfluidic device’s limited processing volume (up to microliters) is critical for real sample pretreatment applications. Here, we have developed a 3D-printed preconcentrator with a pillar structure (3DP2) to enrich the biological samples up to hundreds of microliter scales (700 μL) within 20 min by utilizing ion concentration polarization (ICP). We designed three-dimensional ready-to-pick reservoirs serially connected with a pillar-structured channel to enable large-volume preconcentration by balancing the preconcentrating forces (depletion, electrophoretic, and electroosmotic force) generated by ICP. Using the I-t and I-V curves, we confirmed that ICP performance was enhanced due to a pillar structure’s suppression of the vortex. Finally, we preconcentrated bovine serum albumin (BSA) and micro ribonucleic acid-21 (miRNA-21) two-fold. Moreover, depending on their size and charge, these were concentrated at different locations and could be extracted easily using pipettes. We believe that this study provides a novel strategy for downstream applications. |
| first_indexed | 2024-04-11T14:30:42Z |
| format | Article |
| id | doaj.art-31ff8b6d6a5043b4bde146ec435bcd3e |
| institution | Directory Open Access Journal |
| issn | 2673-5067 |
| language | English |
| last_indexed | 2024-04-11T14:30:42Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sensors |
| spelling | doaj.art-31ff8b6d6a5043b4bde146ec435bcd3e2022-12-22T04:18:38ZengFrontiers Media S.A.Frontiers in Sensors2673-50672022-11-01310.3389/fsens.2022.10669741066974Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applicationsSeungmin Lee0Seungmin Lee1Jinhwan Kim2Na Eun Lee3Na Eun Lee4Kang Hyeon Kim5Seong Jun Park6Jeong Soo Park7Cheonjung Kim8Cheonjung Kim9Ji Hye Hong10Ji Hye Hong11Dae Sung Yoon12Yong Kyoung Yoo13Jeong Hoon Lee14Department of Electrical Engineering, Kwangwoon University, Seoul, South KoreaSchool of Biomedical Engineering, Korea University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaDepartment of Electronic Engineering, Catholic Kwandong University, Gangneung-si, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaSchool of Biomedical Engineering, Korea University, Seoul, South KoreaSchool of Biomedical Engineering, Korea University, Seoul, South KoreaDepartment of Electronic Engineering, Catholic Kwandong University, Gangneung-si, South KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul, South KoreaA sample preconcentration technique that exceeds a microfluidic device’s limited processing volume (up to microliters) is critical for real sample pretreatment applications. Here, we have developed a 3D-printed preconcentrator with a pillar structure (3DP2) to enrich the biological samples up to hundreds of microliter scales (700 μL) within 20 min by utilizing ion concentration polarization (ICP). We designed three-dimensional ready-to-pick reservoirs serially connected with a pillar-structured channel to enable large-volume preconcentration by balancing the preconcentrating forces (depletion, electrophoretic, and electroosmotic force) generated by ICP. Using the I-t and I-V curves, we confirmed that ICP performance was enhanced due to a pillar structure’s suppression of the vortex. Finally, we preconcentrated bovine serum albumin (BSA) and micro ribonucleic acid-21 (miRNA-21) two-fold. Moreover, depending on their size and charge, these were concentrated at different locations and could be extracted easily using pipettes. We believe that this study provides a novel strategy for downstream applications.https://www.frontiersin.org/articles/10.3389/fsens.2022.1066974/fullion concentration polarizationMiRNA-213D printingpreconcentrationelectrokinectic |
| spellingShingle | Seungmin Lee Seungmin Lee Jinhwan Kim Na Eun Lee Na Eun Lee Kang Hyeon Kim Seong Jun Park Jeong Soo Park Cheonjung Kim Cheonjung Kim Ji Hye Hong Ji Hye Hong Dae Sung Yoon Yong Kyoung Yoo Jeong Hoon Lee Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications Frontiers in Sensors ion concentration polarization MiRNA-21 3D printing preconcentration electrokinectic |
| title | Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications |
| title_full | Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications |
| title_fullStr | Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications |
| title_full_unstemmed | Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications |
| title_short | Three-dimensional ready-to-pick reservoir-based preconcentrator with a pillar-structured channel for miRNA applications |
| title_sort | three dimensional ready to pick reservoir based preconcentrator with a pillar structured channel for mirna applications |
| topic | ion concentration polarization MiRNA-21 3D printing preconcentration electrokinectic |
| url | https://www.frontiersin.org/articles/10.3389/fsens.2022.1066974/full |
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