Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia
Preterm birth is the principal contributor to neonatal death and morbidity worldwide. We previously described a plasma cell-free RNA panel that between 16 and 20 weeks of pregnancy had potential to predict spontaneous preterm birth (sPTB) ≤ 32 weeks caused by preterm labor (PTL) or preterm premature...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/6/1327 |
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| author | Carl Philip Weiner Howard Cuckle Mark Louis Weiss Irina Alexandra Buhimschi Yafeng Dong Helen Zhou Risa Ramsey Robert Egerman Catalin Sorin Buhimschi |
| author_facet | Carl Philip Weiner Howard Cuckle Mark Louis Weiss Irina Alexandra Buhimschi Yafeng Dong Helen Zhou Risa Ramsey Robert Egerman Catalin Sorin Buhimschi |
| author_sort | Carl Philip Weiner |
| collection | DOAJ |
| description | Preterm birth is the principal contributor to neonatal death and morbidity worldwide. We previously described a plasma cell-free RNA panel that between 16 and 20 weeks of pregnancy had potential to predict spontaneous preterm birth (sPTB) ≤ 32 weeks caused by preterm labor (PTL) or preterm premature rupture of membranes (PPROM). The present study had three objectives: <b>(1)</b> estimate the RNA panel prognostic accuracy for PTL/PPROM ≤ 32 weeks in a larger series; <b>(2)</b> improve accuracy by adding clinical characteristics to the predictive model; and <b>(3)</b> examine the association of the RNA panel with preeclampsia. We studied 289 women from Memphis TN prospectively sampled 16.0–20.7 weeks and found: (1) <i>PSME2</i> and <i>Hsa-Let 7g</i> were differentially expressed in cases of PTL/PPROM ≤ 32 weeks and together provided fair predictive accuracy with AUC of 0.76; (2) combining the two RNAs with clinical characteristics improved good predictive accuracy for PTL/PPROM ≤ 32 weeks (AUC 0.83); (3) <i>NAMPT</i> and <i>APOA1</i> were differentially expressed in women with ‘early-onset preeclampsia’ (EOP) and together provided good predictive accuracy with AUC of 0.89; and (4) combining the two RNAs with clinical characteristics provided excellent predictive accuracy (AUC 0.96). Our findings suggest an underlying common pathophysiological relationship between PTL/PPROM ≤ 32 weeks and EOP and open inroads for the prognostication of high-risk pregnancies. |
| first_indexed | 2024-03-10T00:01:05Z |
| format | Article |
| id | doaj.art-31ffcd6bbfc24d4b80f992cc85859746 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T00:01:05Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-31ffcd6bbfc24d4b80f992cc858597462023-11-23T16:16:22ZengMDPI AGDiagnostics2075-44182022-05-01126132710.3390/diagnostics12061327Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of PreeclampsiaCarl Philip Weiner0Howard Cuckle1Mark Louis Weiss2Irina Alexandra Buhimschi3Yafeng Dong4Helen Zhou5Risa Ramsey6Robert Egerman7Catalin Sorin Buhimschi8Department of Obstetrics and Gynecology, Kansas University Medical Center, Kansas City, KS 66160, USAFaculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 6934206, IsraelDepartments of Anatomy and Physiology & Midwest Institute of Comparative Stem Cell Biology, Kansas State University, Manhattan, KS 66506, USADepartment of Obstetrics and Gynecology, University of Illinois-Chicago, Chicago, IL 60612, USADepartment of Obstetrics and Gynecology, Kansas University Medical Center, Kansas City, KS 66160, USADepartment of Obstetrics and Gynecology, Kansas University Medical Center, Kansas City, KS 66160, USAOffice of Clinical Research, University of Tennessee Health Science Center, Memphis, TN 38163, USADepartment of Obstetrics and Gynecology, University of Florida, Gainesville, FL 32611, USADepartment of Obstetrics and Gynecology, University of Illinois-Chicago, Chicago, IL 60612, USAPreterm birth is the principal contributor to neonatal death and morbidity worldwide. We previously described a plasma cell-free RNA panel that between 16 and 20 weeks of pregnancy had potential to predict spontaneous preterm birth (sPTB) ≤ 32 weeks caused by preterm labor (PTL) or preterm premature rupture of membranes (PPROM). The present study had three objectives: <b>(1)</b> estimate the RNA panel prognostic accuracy for PTL/PPROM ≤ 32 weeks in a larger series; <b>(2)</b> improve accuracy by adding clinical characteristics to the predictive model; and <b>(3)</b> examine the association of the RNA panel with preeclampsia. We studied 289 women from Memphis TN prospectively sampled 16.0–20.7 weeks and found: (1) <i>PSME2</i> and <i>Hsa-Let 7g</i> were differentially expressed in cases of PTL/PPROM ≤ 32 weeks and together provided fair predictive accuracy with AUC of 0.76; (2) combining the two RNAs with clinical characteristics improved good predictive accuracy for PTL/PPROM ≤ 32 weeks (AUC 0.83); (3) <i>NAMPT</i> and <i>APOA1</i> were differentially expressed in women with ‘early-onset preeclampsia’ (EOP) and together provided good predictive accuracy with AUC of 0.89; and (4) combining the two RNAs with clinical characteristics provided excellent predictive accuracy (AUC 0.96). Our findings suggest an underlying common pathophysiological relationship between PTL/PPROM ≤ 32 weeks and EOP and open inroads for the prognostication of high-risk pregnancies.https://www.mdpi.com/2075-4418/12/6/1327preterm birthextreme prematuritypreterm laborpreeclampsiaearly onset preeclampsiaplasma RNA |
| spellingShingle | Carl Philip Weiner Howard Cuckle Mark Louis Weiss Irina Alexandra Buhimschi Yafeng Dong Helen Zhou Risa Ramsey Robert Egerman Catalin Sorin Buhimschi Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia Diagnostics preterm birth extreme prematurity preterm labor preeclampsia early onset preeclampsia plasma RNA |
| title | Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia |
| title_full | Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia |
| title_fullStr | Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia |
| title_full_unstemmed | Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia |
| title_short | Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia |
| title_sort | evaluation of a maternal plasma rna panel predicting spontaneous preterm birth and its expansion to the prediction of preeclampsia |
| topic | preterm birth extreme prematurity preterm labor preeclampsia early onset preeclampsia plasma RNA |
| url | https://www.mdpi.com/2075-4418/12/6/1327 |
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