Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene
As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1361335/full |
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| author | Qin Li Qiao Zhou Yuan Chen Kaidi Hu Montserrat Sarrà Jianlong Li Aiping Liu Likou Zou Shuliang Liu |
| author_facet | Qin Li Qiao Zhou Yuan Chen Kaidi Hu Montserrat Sarrà Jianlong Li Aiping Liu Likou Zou Shuliang Liu |
| author_sort | Qin Li |
| collection | DOAJ |
| description | As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230 bp). After sca was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4′-HO-3-PBA and 3′-HO-4-PBA. In conclusion, we successfully identified and validated sca as the pivotal enzyme responsible for the efficient degradation of 3-PBA from Sphingomonas baderi, providing a crucial theoretical foundation for further explorations on the degradation potential of SC-1. |
| first_indexed | 2024-04-24T13:09:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T13:09:21Z |
| publishDate | 2024-04-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-3202dc110f6149229600b152d653d0d82024-04-05T04:43:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13613351361335Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading geneQin Li0Qiao Zhou1Yuan Chen2Kaidi Hu3Montserrat Sarrà4Jianlong Li5Aiping Liu6Likou Zou7Shuliang Liu8College of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaDepartament d’Enginyeria Química, Biològica i Ambiental, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, SpainCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an, Sichuan, ChinaAs an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230 bp). After sca was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4′-HO-3-PBA and 3′-HO-4-PBA. In conclusion, we successfully identified and validated sca as the pivotal enzyme responsible for the efficient degradation of 3-PBA from Sphingomonas baderi, providing a crucial theoretical foundation for further explorations on the degradation potential of SC-1.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1361335/fullSphingobium baderi SC-1whole-genome sequencing3-phenoxybenzoic acid-degrading generesting cellsdegradation characteristics |
| spellingShingle | Qin Li Qiao Zhou Yuan Chen Kaidi Hu Montserrat Sarrà Jianlong Li Aiping Liu Likou Zou Shuliang Liu Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene Frontiers in Microbiology Sphingobium baderi SC-1 whole-genome sequencing 3-phenoxybenzoic acid-degrading gene resting cells degradation characteristics |
| title | Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene |
| title_full | Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene |
| title_fullStr | Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene |
| title_full_unstemmed | Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene |
| title_short | Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene |
| title_sort | whole genome sequencing of sphingobium baderi sc 1 and identification of a crucial 3 phenoxybenzoic acid degrading gene |
| topic | Sphingobium baderi SC-1 whole-genome sequencing 3-phenoxybenzoic acid-degrading gene resting cells degradation characteristics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1361335/full |
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