MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro
Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replic...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/12/2/209 |
| _version_ | 1818252409521045504 |
|---|---|
| author | Yan Zhou Linlin Chen Jing Du Xiaoqing Hu Yuping Xie Jinyuan Wu Xiaochen Lin Na Yin Maosheng Sun Hongjun Li |
| author_facet | Yan Zhou Linlin Chen Jing Du Xiaoqing Hu Yuping Xie Jinyuan Wu Xiaochen Lin Na Yin Maosheng Sun Hongjun Li |
| author_sort | Yan Zhou |
| collection | DOAJ |
| description | Rotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus. |
| first_indexed | 2024-12-12T16:23:43Z |
| format | Article |
| id | doaj.art-3205225bdff540edb5c9eb4a7bf39856 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-12T16:23:43Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3205225bdff540edb5c9eb4a7bf398562022-12-22T00:18:56ZengMDPI AGViruses1999-49152020-02-0112220910.3390/v12020209v12020209MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In VitroYan Zhou0Linlin Chen1Jing Du2Xiaoqing Hu3Yuping Xie4Jinyuan Wu5Xiaochen Lin6Na Yin7Maosheng Sun8Hongjun Li9Institute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on severe Infectious Disease, Kunming 650118, ChinaRotavirus (RV) is the major causes of severe diarrhea in infants and young children under five years of age. There are no effective drugs for the treatment of rotavirus in addition to preventive live attenuated vaccine. Recent evidence demonstrates that microRNAs (miRNAs) can affect RNA virus replication. However, the antiviral effect of miRNAs during rotavirus replication are largely unknown. Here, we determined that miR-7 is upregulated during RV replication and that it targets the RV NSP5 (Nonstructural protein 5). Results suggested that miR-7 affected viroplasm formation and inhibited RV replication by down-regulating RV NSP5 expression. Up-regulation of miR-7 expression is a common regulation method of different G-type RV-infected host cells. Then, we further revealed the antiviral effect of miR-7 in diarrhea suckling mice model. MiR-7 is able to inhibit rotavirus replication in vitro and in vivo. These data provide that understanding the role of cellular miR-7 during rotaviral replication may help in the identification of novel therapeutic small RNA molecule drug for anti-rotavirus.https://www.mdpi.com/1999-4915/12/2/209rotavirusmicrorna 7non-structural protein 5gene replicationanti-viral |
| spellingShingle | Yan Zhou Linlin Chen Jing Du Xiaoqing Hu Yuping Xie Jinyuan Wu Xiaochen Lin Na Yin Maosheng Sun Hongjun Li MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro Viruses rotavirus microrna 7 non-structural protein 5 gene replication anti-viral |
| title | MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro |
| title_full | MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro |
| title_fullStr | MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro |
| title_full_unstemmed | MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro |
| title_short | MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro |
| title_sort | microrna 7 inhibits rotavirus replication by targeting viral nsp5 in vivo and in vitro |
| topic | rotavirus microrna 7 non-structural protein 5 gene replication anti-viral |
| url | https://www.mdpi.com/1999-4915/12/2/209 |
| work_keys_str_mv | AT yanzhou microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT linlinchen microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT jingdu microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT xiaoqinghu microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT yupingxie microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT jinyuanwu microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT xiaochenlin microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT nayin microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT maoshengsun microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro AT hongjunli microrna7inhibitsrotavirusreplicationbytargetingviralnsp5invivoandinvitro |