Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout
Lake Mariout is one of the polluted coastal marine ecosystems in Egypt which is considered to be a reservoir of serious effluents from different anthropogenic activities. Such selective pressure enforces indigenous microbial populations to acquire new advantageous themes. Thus, in this study, two &l...
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| author | Marwa Eltarahony Amany Ibrahim Hadeel El-shall Eman Ibrahim Fayez Althobaiti Eman Fayad |
| author_facet | Marwa Eltarahony Amany Ibrahim Hadeel El-shall Eman Ibrahim Fayez Althobaiti Eman Fayad |
| author_sort | Marwa Eltarahony |
| collection | DOAJ |
| description | Lake Mariout is one of the polluted coastal marine ecosystems in Egypt which is considered to be a reservoir of serious effluents from different anthropogenic activities. Such selective pressure enforces indigenous microbial populations to acquire new advantageous themes. Thus, in this study, two <i>Streptomyces</i> strains were screened, from Lake Mariout’s sediment for bioreduction of 5 mM AgNO<sub>3</sub>. Both strains were identified molecularly; their biochemical and physiological characterization revealed their ability to secrete bioactive metabolites with antagonistic activity. The cultural and incubation conditions influencing AgNPs productivity were evaluated. Subsequently, the physicochemical properties of the biofabricated AgNPs were pursued. UV-Vis spectroscopy detected surface plasmon resonance at range 458–422 nm. XRD indicated crystalline, pure, face-centered cubic AgNPs; EDX demonstrated strong silver signal at 3.5 keV. Besides, FT-IR and TGA analysis unveiled self-stabilization and functionalization of AgNPs by bioorganic molecules. However, electron microscopy micrographs depicted numerous uniform spherical AgNPs (1.17–13.3 nm). Potent bactericidal and fungicide activity were recorded by zone of inhibition assay at 50 μg/mL. Further, the antibiofilm activity was exerted in a dose-dependent manner. Moreover, the conjugation of AgNPs with the crude bioactive metabolites of both bionanofactories ameliorated the antimicrobial potency, reflecting a synergistic efficiency versus examined pathogens (free-living and biofilm). |
| first_indexed | 2024-03-10T11:15:56Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:15:56Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-320be72401ed402a9d69e7c22502d74e2023-11-21T20:24:03ZengMDPI AGMolecules1420-30492021-05-012610302710.3390/molecules26103027Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake MarioutMarwa Eltarahony0Amany Ibrahim1Hadeel El-shall2Eman Ibrahim3Fayez Althobaiti4Eman Fayad5Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, EgyptDepartment of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaLake Mariout is one of the polluted coastal marine ecosystems in Egypt which is considered to be a reservoir of serious effluents from different anthropogenic activities. Such selective pressure enforces indigenous microbial populations to acquire new advantageous themes. Thus, in this study, two <i>Streptomyces</i> strains were screened, from Lake Mariout’s sediment for bioreduction of 5 mM AgNO<sub>3</sub>. Both strains were identified molecularly; their biochemical and physiological characterization revealed their ability to secrete bioactive metabolites with antagonistic activity. The cultural and incubation conditions influencing AgNPs productivity were evaluated. Subsequently, the physicochemical properties of the biofabricated AgNPs were pursued. UV-Vis spectroscopy detected surface plasmon resonance at range 458–422 nm. XRD indicated crystalline, pure, face-centered cubic AgNPs; EDX demonstrated strong silver signal at 3.5 keV. Besides, FT-IR and TGA analysis unveiled self-stabilization and functionalization of AgNPs by bioorganic molecules. However, electron microscopy micrographs depicted numerous uniform spherical AgNPs (1.17–13.3 nm). Potent bactericidal and fungicide activity were recorded by zone of inhibition assay at 50 μg/mL. Further, the antibiofilm activity was exerted in a dose-dependent manner. Moreover, the conjugation of AgNPs with the crude bioactive metabolites of both bionanofactories ameliorated the antimicrobial potency, reflecting a synergistic efficiency versus examined pathogens (free-living and biofilm).https://www.mdpi.com/1420-3049/26/10/3027antimicrobialbioactive secondary metabolites<i>Streptomyces</i> sp.nanobiotechnologymarine ecosystemmultidrug resistance |
| spellingShingle | Marwa Eltarahony Amany Ibrahim Hadeel El-shall Eman Ibrahim Fayez Althobaiti Eman Fayad Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout Molecules antimicrobial bioactive secondary metabolites <i>Streptomyces</i> sp. nanobiotechnology marine ecosystem multidrug resistance |
| title | Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout |
| title_full | Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout |
| title_fullStr | Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout |
| title_full_unstemmed | Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout |
| title_short | Antibacterial, Antifungal and Antibiofilm Activities of Silver Nanoparticles Supported by Crude Bioactive Metabolites of Bionanofactories Isolated from Lake Mariout |
| title_sort | antibacterial antifungal and antibiofilm activities of silver nanoparticles supported by crude bioactive metabolites of bionanofactories isolated from lake mariout |
| topic | antimicrobial bioactive secondary metabolites <i>Streptomyces</i> sp. nanobiotechnology marine ecosystem multidrug resistance |
| url | https://www.mdpi.com/1420-3049/26/10/3027 |
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