High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles
Short noncoding (snc)RNAs are important new players in the landscape of biologics with therapeutic potential. Recently, we reported on a new method for the synthesis and delivery of snc RNA in B-cells transfected with plasmid DNA. Here using the same approach, we demonstrate that B-cells can be prog...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-01-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253116300579 |
| _version_ | 1828762529643364352 |
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| author | Gonzalo Almanza Maurizio Zanetti |
| author_facet | Gonzalo Almanza Maurizio Zanetti |
| author_sort | Gonzalo Almanza |
| collection | DOAJ |
| description | Short noncoding (snc)RNAs are important new players in the landscape of biologics with therapeutic potential. Recently, we reported on a new method for the synthesis and delivery of snc RNA in B-cells transfected with plasmid DNA. Here using the same approach, we demonstrate that B-cells can be programmed for the enforced biogenesis and synchronous release of multiple sncRNAs. Our data show that this goal is feasible and that multiple sncRNA are released in the extracellular compartment in amounts comparable to those from B-cells programmed to express and secrete one scnRNA only. Furthermore, we found that the cargo of extracellular vescicles (EVs) isolated from programmed B-cells is remarkably enriched for multiple sncRNA. On average, we found that the content of multiple sncRNAs in EVs is 3.6 copynumber/EV. Collectively, we demonstrate that B-cells can be easily programmed toward the synthesis and release of multiple sncRNAs, including sncRNA-laden EVs, efficiently and specifically. |
| first_indexed | 2024-12-11T01:46:54Z |
| format | Article |
| id | doaj.art-320d9257d11b445a8cd5cf64b8d783fc |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-11T01:46:54Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-320d9257d11b445a8cd5cf64b8d783fc2022-12-22T01:24:53ZengElsevierMolecular Therapy: Nucleic Acids2162-25312015-01-014C10.1038/mtna.2015.44High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular VesiclesGonzalo Almanza0Maurizio Zanetti1The Laboratory of Immunology, Department of Medicine and Moores Cancer Center, University of California, San Diego, California, USAThe Laboratory of Immunology, Department of Medicine and Moores Cancer Center, University of California, San Diego, California, USAShort noncoding (snc)RNAs are important new players in the landscape of biologics with therapeutic potential. Recently, we reported on a new method for the synthesis and delivery of snc RNA in B-cells transfected with plasmid DNA. Here using the same approach, we demonstrate that B-cells can be programmed for the enforced biogenesis and synchronous release of multiple sncRNAs. Our data show that this goal is feasible and that multiple sncRNA are released in the extracellular compartment in amounts comparable to those from B-cells programmed to express and secrete one scnRNA only. Furthermore, we found that the cargo of extracellular vescicles (EVs) isolated from programmed B-cells is remarkably enriched for multiple sncRNA. On average, we found that the content of multiple sncRNAs in EVs is 3.6 copynumber/EV. Collectively, we demonstrate that B-cells can be easily programmed toward the synthesis and release of multiple sncRNAs, including sncRNA-laden EVs, efficiently and specifically.http://www.sciencedirect.com/science/article/pii/S2162253116300579extracellular vesiclesmiRNAnanoparticle tracking analysisquantitative reverse transcription polymerase chain reactionshort noncoding RNAuntranslated region |
| spellingShingle | Gonzalo Almanza Maurizio Zanetti High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles Molecular Therapy: Nucleic Acids extracellular vesicles miRNA nanoparticle tracking analysis quantitative reverse transcription polymerase chain reaction short noncoding RNA untranslated region |
| title | High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles |
| title_full | High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles |
| title_fullStr | High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles |
| title_full_unstemmed | High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles |
| title_short | High-efficiency Generation of Multiple Short Noncoding RNA in B-cells and B-cell-derived Extracellular Vesicles |
| title_sort | high efficiency generation of multiple short noncoding rna in b cells and b cell derived extracellular vesicles |
| topic | extracellular vesicles miRNA nanoparticle tracking analysis quantitative reverse transcription polymerase chain reaction short noncoding RNA untranslated region |
| url | http://www.sciencedirect.com/science/article/pii/S2162253116300579 |
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