Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

Abstract Background A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells with an expression vector carr...

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Bibliographic Details
Main Authors: Maritza Ocampo Betancur, Viviane Castelo Branco Reis, André Moraes Nicola, Janice Lisboa De Marco, Lídia Maria Pepe de Moraes, Fernando Araripe Gonçalves Torres
Format: Article
Language:English
Published: BMC 2017-06-01
Series:Microbial Cell Factories
Subjects:
Komagataella phaffii
Leucine biosynthesis
Auxotrophic marker
Multicopy integration
Expression system
Online Access:http://link.springer.com/article/10.1186/s12934-017-0715-8

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