Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation
Forensic toxicologists often detect lidocaine in the biological fluids of the deceased, due to cardiopulmonary resuscitation (CPR) attempts prior to death. Here, we describe the development of a rapid, sensitive and robust method for the detection of lidocaine in postmortem whole blood using liquid−...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2297-8739/8/8/117 |
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| author | Amvrosios Orfanidis Nikolaos Raikos Evdokia Brousa Eleni Zangelidou Orthodoxia Mastrogianni |
| author_facet | Amvrosios Orfanidis Nikolaos Raikos Evdokia Brousa Eleni Zangelidou Orthodoxia Mastrogianni |
| author_sort | Amvrosios Orfanidis |
| collection | DOAJ |
| description | Forensic toxicologists often detect lidocaine in the biological fluids of the deceased, due to cardiopulmonary resuscitation (CPR) attempts prior to death. Here, we describe the development of a rapid, sensitive and robust method for the detection of lidocaine in postmortem whole blood using liquid−liquid extraction (LLE) followed by GC/MS analysis. The method showed a dynamic linear range of 100 to 6000 ng/mL with a linearity expressed by the regression coefficient (R<sup>2</sup>) and a value of 0.9947. The quantitation limit (LOQ) was found to be 0.03 ng/mL and the detection limit (LOD) 0.01 ng/mL. Recovery accuracy and repeatability were satisfactory. Finally, the method was applied to 23 real whole blood samples from cases where CPR was attempted. Blood concentrations ranged from 0.21–0.96 μg/mL. |
| first_indexed | 2024-03-10T08:23:14Z |
| format | Article |
| id | doaj.art-320fb6ccd3f843eda455bf2846ae4f0b |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T08:23:14Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-320fb6ccd3f843eda455bf2846ae4f0b2023-11-22T09:44:11ZengMDPI AGSeparations2297-87392021-08-018811710.3390/separations8080117Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary ResuscitationAmvrosios Orfanidis0Nikolaos Raikos1Evdokia Brousa2Eleni Zangelidou3Orthodoxia Mastrogianni4Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University, 54124 Thessaloniki, GreeceLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University, 54124 Thessaloniki, GreeceLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University, 54124 Thessaloniki, GreeceLaboratory of Toxicology, Forensic Service of Ministry of Justice, 56334 Thessaloniki, GreeceLaboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University, 54124 Thessaloniki, GreeceForensic toxicologists often detect lidocaine in the biological fluids of the deceased, due to cardiopulmonary resuscitation (CPR) attempts prior to death. Here, we describe the development of a rapid, sensitive and robust method for the detection of lidocaine in postmortem whole blood using liquid−liquid extraction (LLE) followed by GC/MS analysis. The method showed a dynamic linear range of 100 to 6000 ng/mL with a linearity expressed by the regression coefficient (R<sup>2</sup>) and a value of 0.9947. The quantitation limit (LOQ) was found to be 0.03 ng/mL and the detection limit (LOD) 0.01 ng/mL. Recovery accuracy and repeatability were satisfactory. Finally, the method was applied to 23 real whole blood samples from cases where CPR was attempted. Blood concentrations ranged from 0.21–0.96 μg/mL.https://www.mdpi.com/2297-8739/8/8/117forensic scienceforensic toxicologylidocaineresuscitationgas chromatography−mass spectrometryliquid-liquid extraction |
| spellingShingle | Amvrosios Orfanidis Nikolaos Raikos Evdokia Brousa Eleni Zangelidou Orthodoxia Mastrogianni Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation Separations forensic science forensic toxicology lidocaine resuscitation gas chromatography−mass spectrometry liquid-liquid extraction |
| title | Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation |
| title_full | Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation |
| title_fullStr | Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation |
| title_full_unstemmed | Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation |
| title_short | Determination of Lidocaine in Postmortem Whole Blood Samples after Unsuccessful Cardiopulmonary Resuscitation |
| title_sort | determination of lidocaine in postmortem whole blood samples after unsuccessful cardiopulmonary resuscitation |
| topic | forensic science forensic toxicology lidocaine resuscitation gas chromatography−mass spectrometry liquid-liquid extraction |
| url | https://www.mdpi.com/2297-8739/8/8/117 |
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