Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function
For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2008-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000008783907062 |
| _version_ | 1818160148227555328 |
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| author | Hirofumi Noguchi Michiko Ueda Shuji Hayashi Naoya Kobayashi Teru Okitsu Yasuhiro Iwanaga Hideo Nagata Yusuke Nakai Shinichi Matsumoto |
| author_facet | Hirofumi Noguchi Michiko Ueda Shuji Hayashi Naoya Kobayashi Teru Okitsu Yasuhiro Iwanaga Hideo Nagata Yusuke Nakai Shinichi Matsumoto |
| author_sort | Hirofumi Noguchi |
| collection | DOAJ |
| description | For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (–) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (–) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas. |
| first_indexed | 2024-12-11T15:57:16Z |
| format | Article |
| id | doaj.art-3215b62b247148879d4e73de2e4be3fa |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T15:57:16Z |
| publishDate | 2008-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-3215b62b247148879d4e73de2e4be3fa2022-12-22T00:59:24ZengSAGE PublishingCell Transplantation0963-68971555-38922008-01-011710.3727/000000008783907062Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft FunctionHirofumi Noguchi0Michiko Ueda1Shuji Hayashi2Naoya Kobayashi3Teru Okitsu4Yasuhiro Iwanaga5Hideo Nagata6Yusuke Nakai7Shinichi Matsumoto8Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanSecond Department of Surgery, Fujita Health University, Aichi 470-1192, JapanDepartment of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, JapanTransplantation Unit, Kyoto University Hospital, Kyoto 606-8507, JapanTransplantation Unit, Kyoto University Hospital, Kyoto 606-8507, JapanSecond Department of Surgery, Fujita Health University, Aichi 470-1192, JapanTransplantation Unit, Kyoto University Hospital, Kyoto 606-8507, JapanSecond Department of Surgery, Fujita Health University, Aichi 470-1192, JapanFor islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (–) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (–) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas.https://doi.org/10.3727/000000008783907062 |
| spellingShingle | Hirofumi Noguchi Michiko Ueda Shuji Hayashi Naoya Kobayashi Teru Okitsu Yasuhiro Iwanaga Hideo Nagata Yusuke Nakai Shinichi Matsumoto Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function Cell Transplantation |
| title | Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function |
| title_full | Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function |
| title_fullStr | Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function |
| title_full_unstemmed | Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function |
| title_short | Ductal Injection of Preservation Solution Increases Islet Yields in Islet Isolation and Improves Islet Graft Function |
| title_sort | ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function |
| url | https://doi.org/10.3727/000000008783907062 |
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