Properties of protease and lipase from whole and individual organ of viscera from three tuna species

Properties of visceral enzymes from yellowfin tuna (Thunnus albacares), skipjack tuna (Katsuwonus pelamis) and tonggol tuna (Thunnus albacares) were studied. The crude enzymes from viscera of yellowfin tuna and skipjack tuna exhibited the highest activities at pH 10.0 whereas it was at pH 9.0 for th...

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Main Authors: Thiraratana Prachumratana, Poonsuk Prasertsan
Format: Article
Language:English
Published: Prince of Songkla University 2008-04-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://rdo.psu.ac.th/sjst/ejournal/journal/30-Suppl-1/0125-3395-30-S1-77-86.pdf
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author Thiraratana Prachumratana
Poonsuk Prasertsan
author_facet Thiraratana Prachumratana
Poonsuk Prasertsan
author_sort Thiraratana Prachumratana
collection DOAJ
description Properties of visceral enzymes from yellowfin tuna (Thunnus albacares), skipjack tuna (Katsuwonus pelamis) and tonggol tuna (Thunnus albacares) were studied. The crude enzymes from viscera of yellowfin tuna and skipjack tuna exhibited the highest activities at pH 10.0 whereas it was at pH 9.0 for those from viscera of tonggol tuna. The enzymes were the most stable at their optima pH after 120 min incubation. The optimum temperatures for protease and lipase activities were at 50oC and 60oC, respectively but the extracted enzymes were more stable in the temperature range of 37- 40oC. The protease and lipase from spleen were the most thermostable with the half-life of 120 and 90 min at 60oC incubation, respectively. Protease activity from spleen accounted for 45.6% of the total protease activity of the whole tuna viscera.
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spelling doaj.art-32171d5e23b843b7a436c357f4c170c22022-12-22T03:19:37ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952008-04-0130Suppl.17786Properties of protease and lipase from whole and individual organ of viscera from three tuna speciesThiraratana PrachumratanaPoonsuk PrasertsanProperties of visceral enzymes from yellowfin tuna (Thunnus albacares), skipjack tuna (Katsuwonus pelamis) and tonggol tuna (Thunnus albacares) were studied. The crude enzymes from viscera of yellowfin tuna and skipjack tuna exhibited the highest activities at pH 10.0 whereas it was at pH 9.0 for those from viscera of tonggol tuna. The enzymes were the most stable at their optima pH after 120 min incubation. The optimum temperatures for protease and lipase activities were at 50oC and 60oC, respectively but the extracted enzymes were more stable in the temperature range of 37- 40oC. The protease and lipase from spleen were the most thermostable with the half-life of 120 and 90 min at 60oC incubation, respectively. Protease activity from spleen accounted for 45.6% of the total protease activity of the whole tuna viscera.http://rdo.psu.ac.th/sjst/ejournal/journal/30-Suppl-1/0125-3395-30-S1-77-86.pdftuna viscerapropertyproteaselipase
spellingShingle Thiraratana Prachumratana
Poonsuk Prasertsan
Properties of protease and lipase from whole and individual organ of viscera from three tuna species
Songklanakarin Journal of Science and Technology (SJST)
tuna viscera
property
protease
lipase
title Properties of protease and lipase from whole and individual organ of viscera from three tuna species
title_full Properties of protease and lipase from whole and individual organ of viscera from three tuna species
title_fullStr Properties of protease and lipase from whole and individual organ of viscera from three tuna species
title_full_unstemmed Properties of protease and lipase from whole and individual organ of viscera from three tuna species
title_short Properties of protease and lipase from whole and individual organ of viscera from three tuna species
title_sort properties of protease and lipase from whole and individual organ of viscera from three tuna species
topic tuna viscera
property
protease
lipase
url http://rdo.psu.ac.th/sjst/ejournal/journal/30-Suppl-1/0125-3395-30-S1-77-86.pdf
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