| Summary: | The main purpose of this study was to compare the validity of the take-off velocity method (TOV) measured with a force platform (FP) (gold standard) versus the flight time method (FT) in a vertical jump to measure jumping performance or neuromuscular fatigue-overload in professional female football players. For this purpose, we used a FP and a validated smartphone application (APP). A total of eight healthy professional female football players (aged 27.25 ± 6.48 years) participated in this study. All performed three valid trials of a countermovement jump and squat jump and were measured at the same time with the APP and the FP. The results show that there is a lack of validity and reliability between jump height (JH) calculated through the TOV method with the FP and the FT method with the FP (r = 0.028, <i>p</i> > 0.84, intraclass correlation coefficient (ICC) = −0.026) and between the JH measured with the FP through the TOV method and the APP with the FT method (r = 0.116, <i>p</i> > 0.43, ICC = −0.094 (−0.314–0.157)). A significant difference between the JH measured through the TOV with the FP versus the APP (<i>p</i> < 0.05), and a trend between the JH obtained with the FP through the TOV and the FT (<i>p</i> = 0.052) is also shown. Finally, the JH with the FP through the FT and the APP did not differ (<i>p</i> > 0.05). The eta-squared of the one-way ANOVA was η2 = 0.085. It seems that only the TOV measured with a FP could guarantee the accuracy of the jump test in SJ+CMJ and SJ, so it is recommended that high-level sportswomen and men should be assessed with the FP through TOV as gold standard technology to ensure correct performance and/or fatigue-overload control during the sport season.
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