Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis
<p>Abstract</p> <p>Background</p> <p>Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-03-01
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| Series: | Radiation Oncology |
| Online Access: | http://www.ro-journal.com/content/5/1/22 |
| _version_ | 1819117773318520832 |
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| author | Logan Richard M Darby Jocelyn M Stringer Andrea M Bowen Joanne M Gibson Rachel J Ong Zhi Yeoh Ann SJ Keefe Dorothy M |
| author_facet | Logan Richard M Darby Jocelyn M Stringer Andrea M Bowen Joanne M Gibson Rachel J Ong Zhi Yeoh Ann SJ Keefe Dorothy M |
| author_sort | Logan Richard M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity.</p> <p>Methods</p> <p>Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry.</p> <p>Results</p> <p>Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy.</p> <p>Conclusions</p> <p>Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting.</p> |
| first_indexed | 2024-12-22T05:38:18Z |
| format | Article |
| id | doaj.art-321b1a94fe42401ea5c7306abb04e3c4 |
| institution | Directory Open Access Journal |
| issn | 1748-717X |
| language | English |
| last_indexed | 2024-12-22T05:38:18Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Radiation Oncology |
| spelling | doaj.art-321b1a94fe42401ea5c7306abb04e3c42022-12-21T18:37:15ZengBMCRadiation Oncology1748-717X2010-03-01512210.1186/1748-717X-5-22Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositisLogan Richard MDarby Jocelyn MStringer Andrea MBowen Joanne MGibson Rachel JOng ZhiYeoh Ann SJKeefe Dorothy M<p>Abstract</p> <p>Background</p> <p>Mucositis is a toxic side effect of anti-cancer treatments and is a major focus in cancer research. Pro-inflammatory cytokines have previously been implicated in the pathophysiology of chemotherapy-induced gastrointestinal mucositis. However, whether they play a key role in the development of radiotherapy-induced gastrointestinal mucositis is still unknown. Therefore, the aim of the present study was to characterise the expression of pro-inflammatory cytokines in the gastrointestinal tract using a rat model of fractionated radiotherapy-induced toxicity.</p> <p>Methods</p> <p>Thirty six female Dark Agouti rats were randomly assigned into groups and received 2.5 Gys abdominal radiotherapy three times a week over six weeks. Real time PCR was conducted to determine the relative change in mRNA expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF in the jejunum and colon. Protein expression of IL-1β, IL-6 and TNF in the intestinal epithelium was investigated using qualitative immunohistochemistry.</p> <p>Results</p> <p>Radiotherapy-induced sub-acute damage was associated with significantly upregulated IL-1β, IL-6 and TNF mRNA levels in the jejunum and colon. The majority of pro-inflammatory cytokine protein expression in the jejunum and colon exhibited minimal change following fractionated radiotherapy.</p> <p>Conclusions</p> <p>Pro-inflammatory cytokines play a key role in radiotherapy-induced gastrointestinal mucositis in the sub-acute onset setting.</p>http://www.ro-journal.com/content/5/1/22 |
| spellingShingle | Logan Richard M Darby Jocelyn M Stringer Andrea M Bowen Joanne M Gibson Rachel J Ong Zhi Yeoh Ann SJ Keefe Dorothy M Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis Radiation Oncology |
| title | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
| title_full | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
| title_fullStr | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
| title_full_unstemmed | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
| title_short | Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis |
| title_sort | pro inflammatory cytokines play a key role in the development of radiotherapy induced gastrointestinal mucositis |
| url | http://www.ro-journal.com/content/5/1/22 |
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