The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen
Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel techni...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2003-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300018 |
| _version_ | 1797757243966357504 |
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| author | Raquel de Andrade Lima Coêlho Hiroshi Yamasaki Emília Perez Luiz Bezerra de Carvalho Jr |
| author_facet | Raquel de Andrade Lima Coêlho Hiroshi Yamasaki Emília Perez Luiz Bezerra de Carvalho Jr |
| author_sort | Raquel de Andrade Lima Coêlho |
| collection | DOAJ |
| description | Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application. |
| first_indexed | 2024-03-12T18:13:04Z |
| format | Article |
| id | doaj.art-321b91fbf85d4957834591ee3089e43c |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:13:04Z |
| publishDate | 2003-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-321b91fbf85d4957834591ee3089e43c2023-08-02T09:14:13ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602003-04-0198339139310.1590/S0074-02762003000300018The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigenRaquel de Andrade Lima CoêlhoHiroshi YamasakiEmília PerezLuiz Bezerra de Carvalho JrImmunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300018polysiloxanepolyvinyl alcoholrecombinant antigenToxocara canis |
| spellingShingle | Raquel de Andrade Lima Coêlho Hiroshi Yamasaki Emília Perez Luiz Bezerra de Carvalho Jr The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen Memorias do Instituto Oswaldo Cruz polysiloxane polyvinyl alcohol recombinant antigen Toxocara canis |
| title | The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen |
| title_full | The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen |
| title_fullStr | The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen |
| title_full_unstemmed | The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen |
| title_short | The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen |
| title_sort | ue of polysiloxane polyvinyl alcohol beads as solid phase in igg anti toxocara canis detection using a recombinant antigen |
| topic | polysiloxane polyvinyl alcohol recombinant antigen Toxocara canis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000300018 |
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