Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening
Loss of function of dyskerin (DKC1), NOP10 and TIN2 are responsible for different inheritance patterns of Dyskeratosis congenita (DC; ORPHA1775). They are key components of telomerase (DKC1 and NOP10) and shelterin (TIN2), and play an important role in telomere homeostasis. They participate in sever...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Redox Biology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231717306705 |
| _version_ | 1828530130674253824 |
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| author | José Santiago Ibáñez-Cabellos Giselle Pérez-Machado Marta Seco-Cervera Ester Berenguer-Pascual José Luis García-Giménez Federico V. Pallardó |
| author_facet | José Santiago Ibáñez-Cabellos Giselle Pérez-Machado Marta Seco-Cervera Ester Berenguer-Pascual José Luis García-Giménez Federico V. Pallardó |
| author_sort | José Santiago Ibáñez-Cabellos |
| collection | DOAJ |
| description | Loss of function of dyskerin (DKC1), NOP10 and TIN2 are responsible for different inheritance patterns of Dyskeratosis congenita (DC; ORPHA1775). They are key components of telomerase (DKC1 and NOP10) and shelterin (TIN2), and play an important role in telomere homeostasis. They participate in several fundamental cellular processes by contributing to Dyskeratosis congenita through mechanisms that are not fully understood. Presence of oxidative stress was postulated to result from telomerase ablation. However, the resulting disturbed redox status can promote telomere attrition by generating a vicious circle, which promotes cellular senescence. This fact prompted us to study if acute loss of DKC1, NOP10 and TINF2 can promote redox disequilibrium as an early event when telomere shortening has not yet taken place. We generated siRNA-mediated (DKC1, NOP10 and TINF2) cell lines by RNA interference, which was confirmed by mRNA and protein expression analyses. No telomere shortening occurred in any silenced cell line. Depletion of H/ACA ribonucleoproteins DKC1 and NOP10 diminished telomerase activity via TERC down-regulation, and produced alterations in pseudouridylation and ribosomal biogenesis. An increase in the GSSG/GSH ratio, carbonylated proteins and oxidized peroxiredoxin-6 was observed, in addition to MnSOD and TRX1 overexpression in the siRNA DC cells. Likewise, high PARylation levels and high PARP1 protein expression were detected. In contrast, the silenced TINF2 cells did not alter any evaluated oxidative stress marker. Altogether these findings lead us to conclude that loss of DKC1 and NOP10 functions induces oxidative stress in a telomere shortening independent manner. Keywords: Aging, Oxidative stress, Antioxidant, Telomeropathies, DNA damage |
| first_indexed | 2024-12-11T22:18:47Z |
| format | Article |
| id | doaj.art-321ecd096cc14916974d72446432ae5d |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-12-11T22:18:47Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-321ecd096cc14916974d72446432ae5d2022-12-22T00:48:31ZengElsevierRedox Biology2213-23172018-04-0114398408Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shorteningJosé Santiago Ibáñez-Cabellos0Giselle Pérez-Machado1Marta Seco-Cervera2Ester Berenguer-Pascual3José Luis García-Giménez4Federico V. Pallardó5Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain; Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, SpainDepartment of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain; Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, SpainDepartment of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain; Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain; Corresponding authors at: University of Valencia, Faculty of Medicine and Dentistry, Department of Physiology, Av/ Blasco Ibañez, 15, Valencia E46010, Spain.Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain; Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain; Corresponding authors at: University of Valencia, Faculty of Medicine and Dentistry, Department of Physiology, Av/ Blasco Ibañez, 15, Valencia E46010, Spain.Loss of function of dyskerin (DKC1), NOP10 and TIN2 are responsible for different inheritance patterns of Dyskeratosis congenita (DC; ORPHA1775). They are key components of telomerase (DKC1 and NOP10) and shelterin (TIN2), and play an important role in telomere homeostasis. They participate in several fundamental cellular processes by contributing to Dyskeratosis congenita through mechanisms that are not fully understood. Presence of oxidative stress was postulated to result from telomerase ablation. However, the resulting disturbed redox status can promote telomere attrition by generating a vicious circle, which promotes cellular senescence. This fact prompted us to study if acute loss of DKC1, NOP10 and TINF2 can promote redox disequilibrium as an early event when telomere shortening has not yet taken place. We generated siRNA-mediated (DKC1, NOP10 and TINF2) cell lines by RNA interference, which was confirmed by mRNA and protein expression analyses. No telomere shortening occurred in any silenced cell line. Depletion of H/ACA ribonucleoproteins DKC1 and NOP10 diminished telomerase activity via TERC down-regulation, and produced alterations in pseudouridylation and ribosomal biogenesis. An increase in the GSSG/GSH ratio, carbonylated proteins and oxidized peroxiredoxin-6 was observed, in addition to MnSOD and TRX1 overexpression in the siRNA DC cells. Likewise, high PARylation levels and high PARP1 protein expression were detected. In contrast, the silenced TINF2 cells did not alter any evaluated oxidative stress marker. Altogether these findings lead us to conclude that loss of DKC1 and NOP10 functions induces oxidative stress in a telomere shortening independent manner. Keywords: Aging, Oxidative stress, Antioxidant, Telomeropathies, DNA damagehttp://www.sciencedirect.com/science/article/pii/S2213231717306705 |
| spellingShingle | José Santiago Ibáñez-Cabellos Giselle Pérez-Machado Marta Seco-Cervera Ester Berenguer-Pascual José Luis García-Giménez Federico V. Pallardó Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening Redox Biology |
| title | Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| title_full | Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| title_fullStr | Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| title_full_unstemmed | Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| title_short | Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| title_sort | acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening |
| url | http://www.sciencedirect.com/science/article/pii/S2213231717306705 |
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