Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle
<p>Abstract</p> <p>Background</p> <p>Our laboratories have previously reported on the experimental infection of cattle with <it>Mycobacterium avium </it>subsp <it>paratuberculosis </it>(<it>M. paratuberculosis</it>) using an intratons...
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| Format: | Article |
| Language: | English |
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BMC
2008-01-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/6/1/5 |
| _version_ | 1818065975406231552 |
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| author | Stabel Judith R Palmer Mitchell V Waters W Ray Bayles Darrell O Bannantine John P Paustian Michael L |
| author_facet | Stabel Judith R Palmer Mitchell V Waters W Ray Bayles Darrell O Bannantine John P Paustian Michael L |
| author_sort | Stabel Judith R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Our laboratories have previously reported on the experimental infection of cattle with <it>Mycobacterium avium </it>subsp <it>paratuberculosis </it>(<it>M. paratuberculosis</it>) using an intratonsillar infection model. In addition, we have recently developed a partial protein array representing 92 <it>M. paratuberculosis </it>coding sequences. These combined tools have enabled a unique look at the temporal analysis of <it>M. paratuberculosis </it>antigens within the native host. The primary objective of this study was to identify <it>M. paratuberculosis </it>antigens detected by cattle early during infection. A secondary objective was to evaluate the humoral immune response in cattle during the initial year of infection.</p> <p>Results</p> <p>Sera from two experimentally infected cattle, taken pre-inoculation and at day 70, 194 and 321 post infection, identified dynamic antibody reactivity among antigens with some showing an increased response over time and others showing declining levels of reactivity over the same time period. A <it>M. paratuberculosis </it>specific protein, encoded by MAP0862, was strongly detected initially, but the antibody response became weaker with time. The most reactive protein was a putative surface antigen encoded by MAP1087. A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle. Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.</p> <p>Conclusion</p> <p>Collectively these results demonstrate that <it>M. paratuberculosis </it>proteins are detected by sera from experimentally infected cattle as early as 70 days after exposure. These data further suggest at least two antigens may be useful in the early diagnosis of <it>M. paratuberculosis </it>infections. Finally, the construction and use of a protein array in this pilot study has led to a novel approach for discovery of <it>M. paratuberculosis </it>antigens.</p> |
| first_indexed | 2024-12-10T15:00:26Z |
| format | Article |
| id | doaj.art-3222583dad9e409fb01d59876884b0f3 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-10T15:00:26Z |
| publishDate | 2008-01-01 |
| publisher | BMC |
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| series | Proteome Science |
| spelling | doaj.art-3222583dad9e409fb01d59876884b0f32022-12-22T01:44:12ZengBMCProteome Science1477-59562008-01-0161510.1186/1477-5956-6-5Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattleStabel Judith RPalmer Mitchell VWaters W RayBayles Darrell OBannantine John PPaustian Michael L<p>Abstract</p> <p>Background</p> <p>Our laboratories have previously reported on the experimental infection of cattle with <it>Mycobacterium avium </it>subsp <it>paratuberculosis </it>(<it>M. paratuberculosis</it>) using an intratonsillar infection model. In addition, we have recently developed a partial protein array representing 92 <it>M. paratuberculosis </it>coding sequences. These combined tools have enabled a unique look at the temporal analysis of <it>M. paratuberculosis </it>antigens within the native host. The primary objective of this study was to identify <it>M. paratuberculosis </it>antigens detected by cattle early during infection. A secondary objective was to evaluate the humoral immune response in cattle during the initial year of infection.</p> <p>Results</p> <p>Sera from two experimentally infected cattle, taken pre-inoculation and at day 70, 194 and 321 post infection, identified dynamic antibody reactivity among antigens with some showing an increased response over time and others showing declining levels of reactivity over the same time period. A <it>M. paratuberculosis </it>specific protein, encoded by MAP0862, was strongly detected initially, but the antibody response became weaker with time. The most reactive protein was a putative surface antigen encoded by MAP1087. A second protein, MAP1204, implicated in virulence, was also strongly detected by day 70 in both cattle. Subsequent experiments showed that these two proteins were detected with sera from 5 of 9 naturally infected cattle in the subclinical stage of Johne's disease.</p> <p>Conclusion</p> <p>Collectively these results demonstrate that <it>M. paratuberculosis </it>proteins are detected by sera from experimentally infected cattle as early as 70 days after exposure. These data further suggest at least two antigens may be useful in the early diagnosis of <it>M. paratuberculosis </it>infections. Finally, the construction and use of a protein array in this pilot study has led to a novel approach for discovery of <it>M. paratuberculosis </it>antigens.</p>http://www.proteomesci.com/content/6/1/5 |
| spellingShingle | Stabel Judith R Palmer Mitchell V Waters W Ray Bayles Darrell O Bannantine John P Paustian Michael L Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle Proteome Science |
| title | Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle |
| title_full | Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle |
| title_fullStr | Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle |
| title_full_unstemmed | Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle |
| title_short | Early antibody response against <it>Mycobacterium avium </it>subspecies <it>paratuberculosis </it>antigens in subclinical cattle |
| title_sort | early antibody response against it mycobacterium avium it subspecies it paratuberculosis it antigens in subclinical cattle |
| url | http://www.proteomesci.com/content/6/1/5 |
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