Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis
ABSTRACT: Egg production levels in late laying hens are negatively correlated with increasing age. Decreased liver and ovarian function in aging laying hens is accompanied by decreased antioxidant capacity, reproductive hormone levels, and follicular development, resulting in decreased synthesis of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005570 |
| _version_ | 1828094338021720064 |
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| author | Haoyuan Wu Jing Yuan Haixu Yin Bo Jing Chang Sun Ivan Stève Nguepi Tsopmejio Zhouyu Jin Hui Song |
| author_facet | Haoyuan Wu Jing Yuan Haixu Yin Bo Jing Chang Sun Ivan Stève Nguepi Tsopmejio Zhouyu Jin Hui Song |
| author_sort | Haoyuan Wu |
| collection | DOAJ |
| description | ABSTRACT: Egg production levels in late laying hens are negatively correlated with increasing age. Decreased liver and ovarian function in aging laying hens is accompanied by decreased antioxidant capacity, reproductive hormone levels, and follicular development, resulting in decreased synthesis of yolk precursors. The golden needle mushroom (Flammulina velutipes) has been reported to exhibit anti-inflammatory, antioxidant, and hypolipidemic properties. We aimed to reveal the therapeutic effects of F. velutipes stem (FVS) on liver–blood–ovary axis and investigate the underlying mechanisms. A total of 360 sixty-seven-wk-old laying hens were randomized into 4 treatment groups: 1) basal maize–soybean meal diet (CON); 2) basal maize + 20 g/kg FVS (2% FVS); 3) basal maize + 40 g/kg FVS (4% FVS); and 4) basal maize + 60 g/kg FVS (6% FVS). FVS groups demonstrated significantly increased egg production and ovarian development compared with the CON group. The addition of FVS increased the levels of antioxidant enzymes (GSH-Px, T-SOD, and T-AOC) in the liver, serum, and ovaries and decreased malondialdehyde levels by regulating the expression of proteins related to the Keap1-Nrf2/ARE signaling pathway. Additionally, FVS significantly decreased ovarian apoptosis by regulating Bax, Bcl-2, and caspase3 mRNA and protein expression levels. FVS significantly increased the expression levels of estradiol, progesterone, luteinizing hormone, and follicle stimulating hormone and their respective receptors. With increased levels of estradiol transported to the liver through the bloodstream, targeted binding to estrogen receptor (ER)-α and ER-β led to significant increases in ApoVLDL II, ApoB, and VTG II mRNA expression associated with yolk precursor synthesis. FVS decreased the levels of triglyceride and total cholesterol and significantly increased the expression of lipid metabolism, and transport-related mRNAs (FAS, PPAR-a/γ, and MTTP) in the liver. Therefore, the dietary supplementation of FVS can maintain the productive performance of aging laying hens by alleviating the degree of oxidative stress and regulating the transport of functional substances along the liver–blood–ovary axis, thereby improving the synthesis of yolk precursors. |
| first_indexed | 2024-04-11T06:57:33Z |
| format | Article |
| id | doaj.art-322514ea60cd48edbd31c870283cf5d6 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T06:57:33Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-322514ea60cd48edbd31c870283cf5d62022-12-22T04:38:56ZengElsevierPoultry Science0032-57912023-01-011021102261Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axisHaoyuan Wu0Jing Yuan1Haixu Yin2Bo Jing3Chang Sun4Ivan Stève Nguepi Tsopmejio5Zhouyu Jin6Hui Song7School of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, P. R. China; Engineering Research Center of the Chinenese Ministry of Education for Bioreactor and Pharmaceutical Development, Changchun, Jilin, 130118, P. R. China; Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Changchun, Jilin, 130118, P. R. China; Corresponding author:ABSTRACT: Egg production levels in late laying hens are negatively correlated with increasing age. Decreased liver and ovarian function in aging laying hens is accompanied by decreased antioxidant capacity, reproductive hormone levels, and follicular development, resulting in decreased synthesis of yolk precursors. The golden needle mushroom (Flammulina velutipes) has been reported to exhibit anti-inflammatory, antioxidant, and hypolipidemic properties. We aimed to reveal the therapeutic effects of F. velutipes stem (FVS) on liver–blood–ovary axis and investigate the underlying mechanisms. A total of 360 sixty-seven-wk-old laying hens were randomized into 4 treatment groups: 1) basal maize–soybean meal diet (CON); 2) basal maize + 20 g/kg FVS (2% FVS); 3) basal maize + 40 g/kg FVS (4% FVS); and 4) basal maize + 60 g/kg FVS (6% FVS). FVS groups demonstrated significantly increased egg production and ovarian development compared with the CON group. The addition of FVS increased the levels of antioxidant enzymes (GSH-Px, T-SOD, and T-AOC) in the liver, serum, and ovaries and decreased malondialdehyde levels by regulating the expression of proteins related to the Keap1-Nrf2/ARE signaling pathway. Additionally, FVS significantly decreased ovarian apoptosis by regulating Bax, Bcl-2, and caspase3 mRNA and protein expression levels. FVS significantly increased the expression levels of estradiol, progesterone, luteinizing hormone, and follicle stimulating hormone and their respective receptors. With increased levels of estradiol transported to the liver through the bloodstream, targeted binding to estrogen receptor (ER)-α and ER-β led to significant increases in ApoVLDL II, ApoB, and VTG II mRNA expression associated with yolk precursor synthesis. FVS decreased the levels of triglyceride and total cholesterol and significantly increased the expression of lipid metabolism, and transport-related mRNAs (FAS, PPAR-a/γ, and MTTP) in the liver. Therefore, the dietary supplementation of FVS can maintain the productive performance of aging laying hens by alleviating the degree of oxidative stress and regulating the transport of functional substances along the liver–blood–ovary axis, thereby improving the synthesis of yolk precursors.http://www.sciencedirect.com/science/article/pii/S0032579122005570Flammulina velutipes stemliver–blood–ovary signal axisoxidative stressreproductive hormoneyolk precursor synthesis |
| spellingShingle | Haoyuan Wu Jing Yuan Haixu Yin Bo Jing Chang Sun Ivan Stève Nguepi Tsopmejio Zhouyu Jin Hui Song Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis Poultry Science Flammulina velutipes stem liver–blood–ovary signal axis oxidative stress reproductive hormone yolk precursor synthesis |
| title | Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis |
| title_full | Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis |
| title_fullStr | Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis |
| title_full_unstemmed | Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis |
| title_short | Flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver–blood–ovary axis |
| title_sort | flammulina velutipes stem regulates oxidative damage and synthesis of yolk precursors in aging laying hens by regulating the liver blood ovary axis |
| topic | Flammulina velutipes stem liver–blood–ovary signal axis oxidative stress reproductive hormone yolk precursor synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005570 |
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