Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis
ABSTRACT: Necrotic enteritis (NE) is a widespread infectious disease caused by Clostridium perfringens that inflicts major economic losses on the global poultry industry. Due to regulations on antibiotic use in poultry production, there is an urgent need for alternative strategies to mitigate the ne...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123003607 |
| _version_ | 1797676339153600512 |
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| author | D. Goo U.D. Gadde W.K. Kim C.G. Gay E.W. Porta S.W. Jones S. Walker H.S. Lillehoj |
| author_facet | D. Goo U.D. Gadde W.K. Kim C.G. Gay E.W. Porta S.W. Jones S. Walker H.S. Lillehoj |
| author_sort | D. Goo |
| collection | DOAJ |
| description | ABSTRACT: Necrotic enteritis (NE) is a widespread infectious disease caused by Clostridium perfringens that inflicts major economic losses on the global poultry industry. Due to regulations on antibiotic use in poultry production, there is an urgent need for alternative strategies to mitigate the negative effects of NE. This paper presents a passive immunization technology that utilizes hyperimmune egg yolk immunoglobulin Y (IgY) specific to the major immunodominant antigens of C. perfringens. Egg yolk IgYs were generated by immunizing hens with 4 different recombinant C. perfringens antigens, and their protective effects against NE were evaluated in commercial broilers. Six different spray-dried egg powders were produced using recombinant C. perfringens antigens: α-toxin, NE B-like toxin (NetB; EB), elongation factor-Tu (ET), pyruvate:ferredoxin oxidoreductase, a mixture of 4 antigens (EM-1), and a nonimmunized control (EC). The challenged groups were either provided with different egg powders at a 1% level or no egg powders (EN). The NE challenge model based on Eimeria maxima and C. perfringens dual infection was used. In Experiments 1 and 2, the EB and ET groups exhibited increased body weight gain (BWG; P < 0.01), decreased NE lesion scores (P < 0.001), and reduced serum NetB levels (P < 0.01) compared to the EN and EC groups. IgY against NetB significantly reduced Leghorn male hepatocellular cytotoxicity in an in vitro test (P < 0.01). In Experiment 3, the protective effect of the IgYs mixture (EM-2) against C. perfringens antigens (NetB and EFTu) and Eimeria antigens (elongation factor-1-alpha: EF1α and Eimeria profilin: 3-1E) was tested. The EM-2 group showed similar body weight, BWG, and feed intake from d 7 to 22 compared to the NC group (P < 0.05). On d 20, the EM-2 group showed comparable intestinal permeability, NE lesion scores, and jejunal NetB and collagen adhesion protein levels to the NC group (P < 0.05). In conclusion, dietary mixture containing antibodies to NetB and EFTu provides protection against experimental NE in chickens through passive immunization. |
| first_indexed | 2024-03-11T22:27:34Z |
| format | Article |
| id | doaj.art-32254d4f484943a382fa8f8fcc46ec54 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T22:27:34Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-32254d4f484943a382fa8f8fcc46ec542023-09-24T05:13:43ZengElsevierPoultry Science0032-57912023-10-0110210102841Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritisD. Goo0U.D. Gadde1W.K. Kim2C.G. Gay3E.W. Porta4S.W. Jones5S. Walker6H.S. Lillehoj7Department of Poultry Science, University of Georgia, Athens, GA, USAAnimal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, USADepartment of Poultry Science, University of Georgia, Athens, GA, USAOffice of National Program-Animal Health, Agricultural Research Service, USDA, Beltsville, MD, USAArkion Life Sciences, New Castle, DE, USAArkion Life Sciences, New Castle, DE, USAArkion Life Sciences, New Castle, DE, USAAnimal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, USA; Corresponding author:ABSTRACT: Necrotic enteritis (NE) is a widespread infectious disease caused by Clostridium perfringens that inflicts major economic losses on the global poultry industry. Due to regulations on antibiotic use in poultry production, there is an urgent need for alternative strategies to mitigate the negative effects of NE. This paper presents a passive immunization technology that utilizes hyperimmune egg yolk immunoglobulin Y (IgY) specific to the major immunodominant antigens of C. perfringens. Egg yolk IgYs were generated by immunizing hens with 4 different recombinant C. perfringens antigens, and their protective effects against NE were evaluated in commercial broilers. Six different spray-dried egg powders were produced using recombinant C. perfringens antigens: α-toxin, NE B-like toxin (NetB; EB), elongation factor-Tu (ET), pyruvate:ferredoxin oxidoreductase, a mixture of 4 antigens (EM-1), and a nonimmunized control (EC). The challenged groups were either provided with different egg powders at a 1% level or no egg powders (EN). The NE challenge model based on Eimeria maxima and C. perfringens dual infection was used. In Experiments 1 and 2, the EB and ET groups exhibited increased body weight gain (BWG; P < 0.01), decreased NE lesion scores (P < 0.001), and reduced serum NetB levels (P < 0.01) compared to the EN and EC groups. IgY against NetB significantly reduced Leghorn male hepatocellular cytotoxicity in an in vitro test (P < 0.01). In Experiment 3, the protective effect of the IgYs mixture (EM-2) against C. perfringens antigens (NetB and EFTu) and Eimeria antigens (elongation factor-1-alpha: EF1α and Eimeria profilin: 3-1E) was tested. The EM-2 group showed similar body weight, BWG, and feed intake from d 7 to 22 compared to the NC group (P < 0.05). On d 20, the EM-2 group showed comparable intestinal permeability, NE lesion scores, and jejunal NetB and collagen adhesion protein levels to the NC group (P < 0.05). In conclusion, dietary mixture containing antibodies to NetB and EFTu provides protection against experimental NE in chickens through passive immunization.http://www.sciencedirect.com/science/article/pii/S0032579123003607broilerClostridium perfringensegg yolk immunoglobulin Ynecrotic enteritisnecrotic enteritis B-like toxin |
| spellingShingle | D. Goo U.D. Gadde W.K. Kim C.G. Gay E.W. Porta S.W. Jones S. Walker H.S. Lillehoj Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis Poultry Science broiler Clostridium perfringens egg yolk immunoglobulin Y necrotic enteritis necrotic enteritis B-like toxin |
| title | Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis |
| title_full | Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis |
| title_fullStr | Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis |
| title_full_unstemmed | Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis |
| title_short | Hyperimmune egg yolk antibodies developed against Clostridium perfringens antigens protect against necrotic enteritis |
| title_sort | hyperimmune egg yolk antibodies developed against clostridium perfringens antigens protect against necrotic enteritis |
| topic | broiler Clostridium perfringens egg yolk immunoglobulin Y necrotic enteritis necrotic enteritis B-like toxin |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123003607 |
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