Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i>
A multiharmonic quartz crystal microbalance (QCM) has been applied to study the viscoelastic properties of the aptamer-based sensing layers at the surface of a QCM transducer covered by neutravidin following interaction with bacteria <i>Listeria innocua.</i> Addition of bacteria in the c...
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MDPI AG
2021-08-01
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| Series: | Sensors |
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| author | Marek Tatarko Sandro Spagnolo Veronika Oravczová Judit Süle Milan Hun Attila Hucker Tibor Hianik |
| author_facet | Marek Tatarko Sandro Spagnolo Veronika Oravczová Judit Süle Milan Hun Attila Hucker Tibor Hianik |
| author_sort | Marek Tatarko |
| collection | DOAJ |
| description | A multiharmonic quartz crystal microbalance (QCM) has been applied to study the viscoelastic properties of the aptamer-based sensing layers at the surface of a QCM transducer covered by neutravidin following interaction with bacteria <i>Listeria innocua.</i> Addition of bacteria in the concentration range 5 × 10<sup>3</sup>–10<sup>6</sup> CFU/mL resulted in a decrease of resonant frequency and in an increase of dissipation. The frequency decrease has been lower than one would expect considering the dimension of the bacteria. This can be caused by lower penetration depth of the acoustics wave (approximately 120 nm) in comparison with the thickness of the bacterial layer (approximately 500 nm). Addition of <i>E. coli</i> at the surface of neutravidin as well as aptamer layers did not result in significant changes in frequency and dissipation. Using the Kelvin–Voight model the analysis of the viscoelastic properties of the sensing layers was performed and several parameters such as penetration depth, Γ, viscosity coefficient, <i>η</i>, and shear modulus, <i>μ</i>, were determined following various modifications of QCM transducer. The penetration depth decreased following adsorption of the neutravidin layer, which is evidence of the formation of a rigid protein structure. This value did not change significantly following adsorption of aptamers and <i>Listeria innocua</i>. Viscosity coefficient was higher for the neutravidin layer in comparison with the naked QCM transducer in a buffer. However, a further increase of viscosity coefficient took place following attachment of aptamers suggesting their softer structure. The interaction of <i>Listeria innocua</i> with the aptamer layer resulted in slight decrease of viscosity coefficient. The shearing modulus increased for the neutravidin layer and decreased following aptamer adsorption, while a slight increase of <i>µ</i> was observed after the addition of <i>Listeria innocua</i>. |
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| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T08:23:14Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-32283875347d402494b934c5dd839a972023-11-22T09:42:19ZengMDPI AGSensors1424-82202021-08-012116558510.3390/s21165585Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i>Marek Tatarko0Sandro Spagnolo1Veronika Oravczová2Judit Süle3Milan Hun4Attila Hucker5Tibor Hianik6Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská dolina F1, 842 48 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská dolina F1, 842 48 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská dolina F1, 842 48 Bratislava, SlovakiaHungarian Dairy Research Institute Ltd., 9200 Mosonmagyaróvár, HungaryHungarian Dairy Research Institute Ltd., 9200 Mosonmagyaróvár, HungaryHungarian Dairy Research Institute Ltd., 9200 Mosonmagyaróvár, HungaryDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynská dolina F1, 842 48 Bratislava, SlovakiaA multiharmonic quartz crystal microbalance (QCM) has been applied to study the viscoelastic properties of the aptamer-based sensing layers at the surface of a QCM transducer covered by neutravidin following interaction with bacteria <i>Listeria innocua.</i> Addition of bacteria in the concentration range 5 × 10<sup>3</sup>–10<sup>6</sup> CFU/mL resulted in a decrease of resonant frequency and in an increase of dissipation. The frequency decrease has been lower than one would expect considering the dimension of the bacteria. This can be caused by lower penetration depth of the acoustics wave (approximately 120 nm) in comparison with the thickness of the bacterial layer (approximately 500 nm). Addition of <i>E. coli</i> at the surface of neutravidin as well as aptamer layers did not result in significant changes in frequency and dissipation. Using the Kelvin–Voight model the analysis of the viscoelastic properties of the sensing layers was performed and several parameters such as penetration depth, Γ, viscosity coefficient, <i>η</i>, and shear modulus, <i>μ</i>, were determined following various modifications of QCM transducer. The penetration depth decreased following adsorption of the neutravidin layer, which is evidence of the formation of a rigid protein structure. This value did not change significantly following adsorption of aptamers and <i>Listeria innocua</i>. Viscosity coefficient was higher for the neutravidin layer in comparison with the naked QCM transducer in a buffer. However, a further increase of viscosity coefficient took place following attachment of aptamers suggesting their softer structure. The interaction of <i>Listeria innocua</i> with the aptamer layer resulted in slight decrease of viscosity coefficient. The shearing modulus increased for the neutravidin layer and decreased following aptamer adsorption, while a slight increase of <i>µ</i> was observed after the addition of <i>Listeria innocua</i>.https://www.mdpi.com/1424-8220/21/16/5585<i>Listeria innocua</i>biosensorquartz crystal microbalancemulti-harmonic analysisDNA aptamerviscoelastic properties |
| spellingShingle | Marek Tatarko Sandro Spagnolo Veronika Oravczová Judit Süle Milan Hun Attila Hucker Tibor Hianik Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> Sensors <i>Listeria innocua</i> biosensor quartz crystal microbalance multi-harmonic analysis DNA aptamer viscoelastic properties |
| title | Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> |
| title_full | Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> |
| title_fullStr | Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> |
| title_full_unstemmed | Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> |
| title_short | Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with <i>Listeria innocua</i> |
| title_sort | changes of viscoelastic properties of aptamer based sensing layers following interaction with i listeria innocua i |
| topic | <i>Listeria innocua</i> biosensor quartz crystal microbalance multi-harmonic analysis DNA aptamer viscoelastic properties |
| url | https://www.mdpi.com/1424-8220/21/16/5585 |
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