Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species
The Artemisia genus belongs to the Asteraceae family and is used in the treatment of many different diseases such as hepatitis and cancer. So far, around 500 species of Artemisia have been found in different regions of the world. Artemisinin is one of the medicinal compounds found in Artemisia speci...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024024198 |
| _version_ | 1797259846855163904 |
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| author | Bita Jamshidi Alireza Etminan Alimehras Mehrabi Lia Shooshtari Alireza Pour-Aboughadareh |
| author_facet | Bita Jamshidi Alireza Etminan Alimehras Mehrabi Lia Shooshtari Alireza Pour-Aboughadareh |
| author_sort | Bita Jamshidi |
| collection | DOAJ |
| description | The Artemisia genus belongs to the Asteraceae family and is used in the treatment of many different diseases such as hepatitis and cancer. So far, around 500 species of Artemisia have been found in different regions of the world. Artemisinin is one of the medicinal compounds found in Artemisia species. Hence, this medical feature encourages researchers to pay attention to various species of this genus to discover more genetic and phytochemical information. In the present study, five species of Artemisia including A. fragrans, A. annua, A. biennis, A. scoparia, and A. absinthium were compared to each other in terms of the artemisinin content and other phytochemical components. Moreover, the relative expression profiles of eight genes related to the accumulation and synthesis of artemisinin [including 4FPSF, DBR2, HMGR1, HMGR2, WIRKY, ADS, DXS, and SQS] were determined in investigated species. The result of high-performance liquid chromatography (HPLC) analysis showed that the content of artemisinin in various species was in the order of A. fragrans > A. annua > A. biennis > A. scoparia > A. absinthium. Based on the gas chromatography–mass spectrometry (GC-MS) analysis, 34, 26, 26, 24, and 20 phytochemical compounds were identified for A. scoparia, A. biennis, A. fragrans, A. absinthum, and A. annua species, respectively. Moreover, camphor (38.86%), β-thujone (68.42%), spathulenol (48.33%), β-farnesene (48.16%), and camphor (29.04%) were identified as the considerable compounds A. fragrans, A. absinthium, A. scoparia, A. biennis, and A. annua species, respectively. Considering the relative expression of the targeted genes, A. scoparia revealed higher expression for the 4FPSF gene. The highest relative expression of the DBR2, WIRKY, and SQS genes was found in A. absinthium species. Moreover, A. annua showed the highest expression of the ADS and DXS genes than the other species. In conclusion, our findings revealed that various species of Artemisia have interesting breeding potential for further investigation of different aspects such as medicinal properties and molecular studies. |
| first_indexed | 2024-03-07T19:41:21Z |
| format | Article |
| id | doaj.art-322a81e3893e4232883a1750b87b7c99 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:15:55Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-322a81e3893e4232883a1750b87b7c992024-03-17T07:55:23ZengElsevierHeliyon2405-84402024-03-01105e26388Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia speciesBita Jamshidi0Alireza Etminan1Alimehras Mehrabi2Lia Shooshtari3Alireza Pour-Aboughadareh4Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, IranDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran; Corresponding author.Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, IranDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, IranSeed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranThe Artemisia genus belongs to the Asteraceae family and is used in the treatment of many different diseases such as hepatitis and cancer. So far, around 500 species of Artemisia have been found in different regions of the world. Artemisinin is one of the medicinal compounds found in Artemisia species. Hence, this medical feature encourages researchers to pay attention to various species of this genus to discover more genetic and phytochemical information. In the present study, five species of Artemisia including A. fragrans, A. annua, A. biennis, A. scoparia, and A. absinthium were compared to each other in terms of the artemisinin content and other phytochemical components. Moreover, the relative expression profiles of eight genes related to the accumulation and synthesis of artemisinin [including 4FPSF, DBR2, HMGR1, HMGR2, WIRKY, ADS, DXS, and SQS] were determined in investigated species. The result of high-performance liquid chromatography (HPLC) analysis showed that the content of artemisinin in various species was in the order of A. fragrans > A. annua > A. biennis > A. scoparia > A. absinthium. Based on the gas chromatography–mass spectrometry (GC-MS) analysis, 34, 26, 26, 24, and 20 phytochemical compounds were identified for A. scoparia, A. biennis, A. fragrans, A. absinthum, and A. annua species, respectively. Moreover, camphor (38.86%), β-thujone (68.42%), spathulenol (48.33%), β-farnesene (48.16%), and camphor (29.04%) were identified as the considerable compounds A. fragrans, A. absinthium, A. scoparia, A. biennis, and A. annua species, respectively. Considering the relative expression of the targeted genes, A. scoparia revealed higher expression for the 4FPSF gene. The highest relative expression of the DBR2, WIRKY, and SQS genes was found in A. absinthium species. Moreover, A. annua showed the highest expression of the ADS and DXS genes than the other species. In conclusion, our findings revealed that various species of Artemisia have interesting breeding potential for further investigation of different aspects such as medicinal properties and molecular studies.http://www.sciencedirect.com/science/article/pii/S2405844024024198Medicinal plantsArtemisiaArtemisininChromatographyGene expression |
| spellingShingle | Bita Jamshidi Alireza Etminan Alimehras Mehrabi Lia Shooshtari Alireza Pour-Aboughadareh Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species Heliyon Medicinal plants Artemisia Artemisinin Chromatography Gene expression |
| title | Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species |
| title_full | Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species |
| title_fullStr | Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species |
| title_full_unstemmed | Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species |
| title_short | Comparison of phytochemical properties and expressional profiling of artemisinin synthesis-related genes in various Artemisia species |
| title_sort | comparison of phytochemical properties and expressional profiling of artemisinin synthesis related genes in various artemisia species |
| topic | Medicinal plants Artemisia Artemisinin Chromatography Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024024198 |
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