Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips
Praziquantel (PZQ) is a broad-spectrum antiparasitic drug used in mammals and fish. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of PZQ residues. The hapten PZQ-HS produced by introducing an amino group at a benzene ring synthesiz...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1641068 |
| _version_ | 1819149793771913216 |
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| author | Xinyi Shen Liqiang Liu Liguang Xu Wei Ma Xiaoling Wu Gang Cui Hua Kuang |
| author_facet | Xinyi Shen Liqiang Liu Liguang Xu Wei Ma Xiaoling Wu Gang Cui Hua Kuang |
| author_sort | Xinyi Shen |
| collection | DOAJ |
| description | Praziquantel (PZQ) is a broad-spectrum antiparasitic drug used in mammals and fish. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of PZQ residues. The hapten PZQ-HS produced by introducing an amino group at a benzene ring synthesized the immunogen and the coating antigen with carrier proteins. Highly sensitive monoclonal antibodies (mAbs) were prepared following mouse immunization and cell fusion. Under optimum conditions, the half maximal inhibitory concentration of the cell line 4E9 was 7.4 ng/mL, and the working range was 2.4–22.8 ng/mL. Immunochromatographic strips (ICS) were developed for the rapid detection of PZQ residues in mackerel. The reliability of the ICS assay was verified with PZQ-negative mackerel and confirmed by HPLC/MS. The cutoff value for ICS was 50 ng/mL PZQ. Our findings revealed that ic-ELISA and ICS can be used for the rapid detection of PZQ in mackerel. |
| first_indexed | 2024-12-22T14:07:15Z |
| format | Article |
| id | doaj.art-322d7511ab0e43a8bbc6de83aa4c87b2 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-22T14:07:15Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-322d7511ab0e43a8bbc6de83aa4c87b22022-12-21T18:23:16ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-0130191392310.1080/09540105.2019.16410681641068Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic stripsXinyi Shen0Liqiang Liu1Liguang Xu2Wei Ma3Xiaoling Wu4Gang Cui5Hua Kuang6Jiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityYancheng Teachers UniversityJiangnan UniversityPraziquantel (PZQ) is a broad-spectrum antiparasitic drug used in mammals and fish. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of PZQ residues. The hapten PZQ-HS produced by introducing an amino group at a benzene ring synthesized the immunogen and the coating antigen with carrier proteins. Highly sensitive monoclonal antibodies (mAbs) were prepared following mouse immunization and cell fusion. Under optimum conditions, the half maximal inhibitory concentration of the cell line 4E9 was 7.4 ng/mL, and the working range was 2.4–22.8 ng/mL. Immunochromatographic strips (ICS) were developed for the rapid detection of PZQ residues in mackerel. The reliability of the ICS assay was verified with PZQ-negative mackerel and confirmed by HPLC/MS. The cutoff value for ICS was 50 ng/mL PZQ. Our findings revealed that ic-ELISA and ICS can be used for the rapid detection of PZQ in mackerel.http://dx.doi.org/10.1080/09540105.2019.1641068praziquantelic-elisaimmunochromatographic stripsmonoclonal antibodymackerel |
| spellingShingle | Xinyi Shen Liqiang Liu Liguang Xu Wei Ma Xiaoling Wu Gang Cui Hua Kuang Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips Food and Agricultural Immunology praziquantel ic-elisa immunochromatographic strips monoclonal antibody mackerel |
| title | Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips |
| title_full | Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips |
| title_fullStr | Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips |
| title_full_unstemmed | Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips |
| title_short | Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips |
| title_sort | rapid detection of praziquantel using monoclonal antibody based ic elisa and immunochromatographic strips |
| topic | praziquantel ic-elisa immunochromatographic strips monoclonal antibody mackerel |
| url | http://dx.doi.org/10.1080/09540105.2019.1641068 |
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