Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo
The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2011-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200022 |
| _version_ | 1828375391007408128 |
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| author | Sanja Matic Snezana Stanic Desanka Bogojevic Slavica Solujic Nevena Grdovic Melita Vidakovic Mirjana Mihailovic |
| author_facet | Sanja Matic Snezana Stanic Desanka Bogojevic Slavica Solujic Nevena Grdovic Melita Vidakovic Mirjana Mihailovic |
| author_sort | Sanja Matic |
| collection | DOAJ |
| description | The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were not significantly different from the control group, whereas in the groups of animals, under the same conditions, but with 1000 and 2000 mg/kg bw of the extract, scores were statistically so. A slight decrease in the comet score and tail moment observed in all the doses in the 72 h treatment, gave to understand that DNA damage induced by Cotinus coggygria extract decreased with time. The results of both tests revealed the genotoxic effect of Cotinus coggygria under our experimental conditions. |
| first_indexed | 2024-04-14T07:46:54Z |
| format | Article |
| id | doaj.art-322e0dcc948742b894bda7b555d052a5 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-14T07:46:54Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-322e0dcc948742b894bda7b555d052a52022-12-22T02:05:19ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-01342298303Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivoSanja MaticSnezana StanicDesanka BogojevicSlavica SolujicNevena GrdovicMelita VidakovicMirjana MihailovicThe intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were not significantly different from the control group, whereas in the groups of animals, under the same conditions, but with 1000 and 2000 mg/kg bw of the extract, scores were statistically so. A slight decrease in the comet score and tail moment observed in all the doses in the 72 h treatment, gave to understand that DNA damage induced by Cotinus coggygria extract decreased with time. The results of both tests revealed the genotoxic effect of Cotinus coggygria under our experimental conditions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200022comet assayCotinus coggygriaextractgenotoxic effectSLRL |
| spellingShingle | Sanja Matic Snezana Stanic Desanka Bogojevic Slavica Solujic Nevena Grdovic Melita Vidakovic Mirjana Mihailovic Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo Genetics and Molecular Biology comet assay Cotinus coggygria extract genotoxic effect SLRL |
| title | Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo |
| title_full | Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo |
| title_fullStr | Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo |
| title_full_unstemmed | Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo |
| title_short | Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo |
| title_sort | genotoxic potential of cotinus coggygria scop anacardiaceae stem extract in vivo |
| topic | comet assay Cotinus coggygria extract genotoxic effect SLRL |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200022 |
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