Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl
Valerenic acid (VA) is a sesquiterpenoid and a phytoconstituent of the plant valerian used for sleeping disorders and anxiety. The frequency of using herbal components as therapeutic nutritional agents has increased lately. Their ability to improve redox homeostasis makes them a valuable approach ag...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/746 |
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| author | Mehtap Kara Ezgi Öztaş Tuğçe Boran Ecem Fatma Karaman Aristidis S. Veskoukis Aristides M. Tsatsakis |
| author_facet | Mehtap Kara Ezgi Öztaş Tuğçe Boran Ecem Fatma Karaman Aristidis S. Veskoukis Aristides M. Tsatsakis |
| author_sort | Mehtap Kara |
| collection | DOAJ |
| description | Valerenic acid (VA) is a sesquiterpenoid and a phytoconstituent of the plant valerian used for sleeping disorders and anxiety. The frequency of using herbal components as therapeutic nutritional agents has increased lately. Their ability to improve redox homeostasis makes them a valuable approach against harmful xenobiotics. The purpose of this study was to evaluate the putative beneficial role of VA against the redox-perturbating role of the fungicide benomyl in HepG2 human liver cells in terms of oxidative stress in the cellular environment and in endoplasmic reticulum (ER). Benomyl increased cell total oxidant status and reactive oxygen species production and decreased total antioxidant status. The expression of genes coding for antioxidant molecules, namely, heme oxygenase-1, alpha glutathione s-transferase, NF-ĸB, and liver fatty acid binding protein, were decreased due to benomyl. VA ameliorated these effects. Benomyl also increased ER-stress-related molecules such as endoplasmic reticulum to nucleus signaling 1 protein, glucose-regulated protein 78, and caspase-12 levels, and VA acted also as a preventive agent. These results indicate that VA exerts ameliorative effects after benomyl-induced oxidative stress. VA, a widely used nutritional supplement, is a compound with potent antioxidant properties, which are valuable for the protection of cells against xenobiotic-induced oxidative damage. |
| first_indexed | 2024-03-10T11:37:20Z |
| format | Article |
| id | doaj.art-32337636796f4fdd82b252ab429461ee |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:37:20Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-32337636796f4fdd82b252ab429461ee2023-11-21T18:45:42ZengMDPI AGAntioxidants2076-39212021-05-0110574610.3390/antiox10050746Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide BenomylMehtap Kara0Ezgi Öztaş1Tuğçe Boran2Ecem Fatma Karaman3Aristidis S. Veskoukis4Aristides M. Tsatsakis5Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul 34116, TurkeyDepartment of Nutrition and Dietetics, School of Physical Education, Sport Science and Dietetics, University of Thessaly, Argonafton 1, 42132 Trikala, GreeceCenter of Toxicology Science and Research, Medical School, University of Crete, 71003 Heraklion, GreeceValerenic acid (VA) is a sesquiterpenoid and a phytoconstituent of the plant valerian used for sleeping disorders and anxiety. The frequency of using herbal components as therapeutic nutritional agents has increased lately. Their ability to improve redox homeostasis makes them a valuable approach against harmful xenobiotics. The purpose of this study was to evaluate the putative beneficial role of VA against the redox-perturbating role of the fungicide benomyl in HepG2 human liver cells in terms of oxidative stress in the cellular environment and in endoplasmic reticulum (ER). Benomyl increased cell total oxidant status and reactive oxygen species production and decreased total antioxidant status. The expression of genes coding for antioxidant molecules, namely, heme oxygenase-1, alpha glutathione s-transferase, NF-ĸB, and liver fatty acid binding protein, were decreased due to benomyl. VA ameliorated these effects. Benomyl also increased ER-stress-related molecules such as endoplasmic reticulum to nucleus signaling 1 protein, glucose-regulated protein 78, and caspase-12 levels, and VA acted also as a preventive agent. These results indicate that VA exerts ameliorative effects after benomyl-induced oxidative stress. VA, a widely used nutritional supplement, is a compound with potent antioxidant properties, which are valuable for the protection of cells against xenobiotic-induced oxidative damage.https://www.mdpi.com/2076-3921/10/5/746valerenic acidvalerianbenomyloxidative stressHepG2 cellsendoplasmic reticulum |
| spellingShingle | Mehtap Kara Ezgi Öztaş Tuğçe Boran Ecem Fatma Karaman Aristidis S. Veskoukis Aristides M. Tsatsakis Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl Antioxidants valerenic acid valerian benomyl oxidative stress HepG2 cells endoplasmic reticulum |
| title | Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl |
| title_full | Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl |
| title_fullStr | Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl |
| title_full_unstemmed | Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl |
| title_short | Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl |
| title_sort | ameliorative effects of the sesquiterpenoid valerenic acid on oxidative stress induced in hepg2 cells after exposure to the fungicide benomyl |
| topic | valerenic acid valerian benomyl oxidative stress HepG2 cells endoplasmic reticulum |
| url | https://www.mdpi.com/2076-3921/10/5/746 |
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