Fungi associated with the potato taste defect in coffee beans from Rwanda
Abstract Background Potato taste defect (PTD) of coffee is characterized by a raw potato like smell that leads to a lower quality taste in the brewed coffee, and harms the commercial value of some East African coffees. Although several causes for PTD have been proposed, none of them have been confir...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-05-01
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| Series: | Botanical Studies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40529-022-00346-9 |
| _version_ | 1818238904077123584 |
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| author | Amanda R. Hale Paul M. Ruegger Philippe Rolshausen James Borneman Jiue-in Yang |
| author_facet | Amanda R. Hale Paul M. Ruegger Philippe Rolshausen James Borneman Jiue-in Yang |
| author_sort | Amanda R. Hale |
| collection | DOAJ |
| description | Abstract Background Potato taste defect (PTD) of coffee is characterized by a raw potato like smell that leads to a lower quality taste in the brewed coffee, and harms the commercial value of some East African coffees. Although several causes for PTD have been proposed, none of them have been confirmed. Recently, high throughput sequencing techniques and bioinformatic analysis have shown great potential for identifying putative causal agents of plant diseases. Toward the goal of determining the cause of PTD, we examined raw coffee beans from Rwanda exhibiting varying PTD scores using an Illumina-based sequence analysis of the fungal rRNA ITS region. Results Six fungal amplicon sequence variants (ASVs) with high relative abundances correlated with coffee taste scores. Four of these ASVs exhibited negative correlations – Aspergillus versicolor, Penicillium cinnamopurpureum , Talaromyces radicus, and Thermomyces lanuginosus – indicating that they might be causing PTD. Two of these fungi exhibited positive correlations – Kazachstania humilis and Clavispora lusitaniae – indicating that they might be inhibiting organisms that cause PTD. Conclusions This study addressed PTD causality from a new angle by examining fungi with high throughput sequencing. To our knowledge, this is the first study characterizing fungi associated with PTD, providing candidates for both causality and biocontrol. |
| first_indexed | 2024-12-12T12:49:03Z |
| format | Article |
| id | doaj.art-3238ab089a1e4011bb2b2375d2b68c91 |
| institution | Directory Open Access Journal |
| issn | 1999-3110 |
| language | English |
| last_indexed | 2024-12-12T12:49:03Z |
| publishDate | 2022-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Botanical Studies |
| spelling | doaj.art-3238ab089a1e4011bb2b2375d2b68c912022-12-22T00:24:03ZengSpringerOpenBotanical Studies1999-31102022-05-016311810.1186/s40529-022-00346-9Fungi associated with the potato taste defect in coffee beans from RwandaAmanda R. Hale0Paul M. Ruegger1Philippe Rolshausen2James Borneman3Jiue-in Yang4Department of Microbiology and Plant Pathology, University of CaliforniaDepartment of Microbiology and Plant Pathology, University of CaliforniaDepartment of Botany and Plant Sciences, University of CaliforniaDepartment of Microbiology and Plant Pathology, University of CaliforniaDepartment of Plant Pathology and Microbiology, National Taiwan UniversityAbstract Background Potato taste defect (PTD) of coffee is characterized by a raw potato like smell that leads to a lower quality taste in the brewed coffee, and harms the commercial value of some East African coffees. Although several causes for PTD have been proposed, none of them have been confirmed. Recently, high throughput sequencing techniques and bioinformatic analysis have shown great potential for identifying putative causal agents of plant diseases. Toward the goal of determining the cause of PTD, we examined raw coffee beans from Rwanda exhibiting varying PTD scores using an Illumina-based sequence analysis of the fungal rRNA ITS region. Results Six fungal amplicon sequence variants (ASVs) with high relative abundances correlated with coffee taste scores. Four of these ASVs exhibited negative correlations – Aspergillus versicolor, Penicillium cinnamopurpureum , Talaromyces radicus, and Thermomyces lanuginosus – indicating that they might be causing PTD. Two of these fungi exhibited positive correlations – Kazachstania humilis and Clavispora lusitaniae – indicating that they might be inhibiting organisms that cause PTD. Conclusions This study addressed PTD causality from a new angle by examining fungi with high throughput sequencing. To our knowledge, this is the first study characterizing fungi associated with PTD, providing candidates for both causality and biocontrol.https://doi.org/10.1186/s40529-022-00346-9High-throughput sequencingrRNA ITS regionAmplicon sequencingMicrobiome |
| spellingShingle | Amanda R. Hale Paul M. Ruegger Philippe Rolshausen James Borneman Jiue-in Yang Fungi associated with the potato taste defect in coffee beans from Rwanda Botanical Studies High-throughput sequencing rRNA ITS region Amplicon sequencing Microbiome |
| title | Fungi associated with the potato taste defect in coffee beans from Rwanda |
| title_full | Fungi associated with the potato taste defect in coffee beans from Rwanda |
| title_fullStr | Fungi associated with the potato taste defect in coffee beans from Rwanda |
| title_full_unstemmed | Fungi associated with the potato taste defect in coffee beans from Rwanda |
| title_short | Fungi associated with the potato taste defect in coffee beans from Rwanda |
| title_sort | fungi associated with the potato taste defect in coffee beans from rwanda |
| topic | High-throughput sequencing rRNA ITS region Amplicon sequencing Microbiome |
| url | https://doi.org/10.1186/s40529-022-00346-9 |
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