Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains
Different inbred mouse strains vary substantially in their behavior and metabolic phenotype under physiological and pathological conditions. The purpose of this study was to extend the knowledge of distinct coping strategies under challenging events in two differently adapting mouse strains: C57BL/6...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11682 |
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| author | Maria Piirsalu Egon Taalberg Mohan Jayaram Kersti Lilleväli Mihkel Zilmer Eero Vasar |
| author_facet | Maria Piirsalu Egon Taalberg Mohan Jayaram Kersti Lilleväli Mihkel Zilmer Eero Vasar |
| author_sort | Maria Piirsalu |
| collection | DOAJ |
| description | Different inbred mouse strains vary substantially in their behavior and metabolic phenotype under physiological and pathological conditions. The purpose of this study was to extend the knowledge of distinct coping strategies under challenging events in two differently adapting mouse strains: C57BL/6NTac (Bl6) and 129S6/SvEvTac (129Sv). Thus, we aimed to investigate possible similarities and differences in the body weight change, behavior, and several metabolic variables in Bl6 and 129Sv strains in response to high-fat diet (HFD) using the AbsoluteIDQ p180 kit. We found that 9 weeks of HFD induced a significant body weight gain in 129Sv, but not in Bl6 mice. Besides that, 129Sv mice displayed anxiety-like behavior in the open-field test. Metabolite profiling revealed that 129Sv mice had higher levels of circulating branched-chain amino acids, which were even more amplified by HFD. HFD also induced a decrease in glycine, spermidine, and t4-OH-proline levels in 129Sv mice. Although acylcarnitines (ACs) dominated in baseline conditions in 129Sv strain, this strain had a significantly stronger AC-reducing effect of HFD. Moreover, 129Sv mice had higher levels of lipids in baseline conditions, but HFD caused more pronounced alterations in lipid profile in Bl6 mice. Taken together, our results show that the Bl6 line is better adapted to abundant fat intake. |
| first_indexed | 2024-03-09T21:38:02Z |
| format | Article |
| id | doaj.art-3239c5db7275453e91edc42198308dd2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:38:02Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3239c5db7275453e91edc42198308dd22023-11-23T20:37:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191168210.3390/ijms231911682Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse StrainsMaria Piirsalu0Egon Taalberg1Mohan Jayaram2Kersti Lilleväli3Mihkel Zilmer4Eero Vasar5Institute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaCenter of Excellence for Genomics and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaInstitute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaInstitute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaCenter of Excellence for Genomics and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaInstitute of Biomedicine and Translational Medicine, Department of Physiology, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDifferent inbred mouse strains vary substantially in their behavior and metabolic phenotype under physiological and pathological conditions. The purpose of this study was to extend the knowledge of distinct coping strategies under challenging events in two differently adapting mouse strains: C57BL/6NTac (Bl6) and 129S6/SvEvTac (129Sv). Thus, we aimed to investigate possible similarities and differences in the body weight change, behavior, and several metabolic variables in Bl6 and 129Sv strains in response to high-fat diet (HFD) using the AbsoluteIDQ p180 kit. We found that 9 weeks of HFD induced a significant body weight gain in 129Sv, but not in Bl6 mice. Besides that, 129Sv mice displayed anxiety-like behavior in the open-field test. Metabolite profiling revealed that 129Sv mice had higher levels of circulating branched-chain amino acids, which were even more amplified by HFD. HFD also induced a decrease in glycine, spermidine, and t4-OH-proline levels in 129Sv mice. Although acylcarnitines (ACs) dominated in baseline conditions in 129Sv strain, this strain had a significantly stronger AC-reducing effect of HFD. Moreover, 129Sv mice had higher levels of lipids in baseline conditions, but HFD caused more pronounced alterations in lipid profile in Bl6 mice. Taken together, our results show that the Bl6 line is better adapted to abundant fat intake.https://www.mdpi.com/1422-0067/23/19/11682129S6/SvEvTacC57BL/6NTacmetabolomicsstandard diethigh-fat diet |
| spellingShingle | Maria Piirsalu Egon Taalberg Mohan Jayaram Kersti Lilleväli Mihkel Zilmer Eero Vasar Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains International Journal of Molecular Sciences 129S6/SvEvTac C57BL/6NTac metabolomics standard diet high-fat diet |
| title | Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains |
| title_full | Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains |
| title_fullStr | Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains |
| title_full_unstemmed | Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains |
| title_short | Impact of a High-Fat Diet on the Metabolomics Profile of 129S6 and C57BL6 Mouse Strains |
| title_sort | impact of a high fat diet on the metabolomics profile of 129s6 and c57bl6 mouse strains |
| topic | 129S6/SvEvTac C57BL/6NTac metabolomics standard diet high-fat diet |
| url | https://www.mdpi.com/1422-0067/23/19/11682 |
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