The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput
To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with the read-out or the reagent of the assay needs to be addressed to avoid biased r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Toxicology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2022.983316/full |
| _version_ | 1798037573484937216 |
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| author | Elise Rundén-Pran Espen Mariussen Espen Mariussen Naouale El Yamani Elisabeth Elje Elisabeth Elje Eleonora Marta Longhin Maria Dusinska |
| author_facet | Elise Rundén-Pran Espen Mariussen Espen Mariussen Naouale El Yamani Elisabeth Elje Elisabeth Elje Eleonora Marta Longhin Maria Dusinska |
| author_sort | Elise Rundén-Pran |
| collection | DOAJ |
| description | To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with the read-out or the reagent of the assay needs to be addressed to avoid biased results. Thus, validated label-free methods are urgently needed for hazard identification of NMs to avoid unintended adverse effects on human health. The colony forming efficiency (CFE) assay is a label- and interference-free method for quantification of cytotoxicity by cell survival and colony forming efficiency by CFE formation. The CFE has shown to be compatible with toxicity testing of NMs. Here we present an optimized protocol for a higher-throughput set up. |
| first_indexed | 2024-04-11T21:28:23Z |
| format | Article |
| id | doaj.art-323d16bd0a264659a179d9d682a3f63d |
| institution | Directory Open Access Journal |
| issn | 2673-3080 |
| language | English |
| last_indexed | 2024-04-11T21:28:23Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Toxicology |
| spelling | doaj.art-323d16bd0a264659a179d9d682a3f63d2022-12-22T04:02:19ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802022-09-01410.3389/ftox.2022.983316983316The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughputElise Rundén-Pran0Espen Mariussen1Espen Mariussen2Naouale El Yamani3Elisabeth Elje4Elisabeth Elje5Eleonora Marta Longhin6Maria Dusinska7Health Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayHealth Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayNorwegian Institute of Public Health, Department for Environmental Chemistry, Department of Air Quality and Noise, Oslo, NorwayHealth Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayHealth Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayUniversity of Oslo, Faculty of Medicine, Institute of Basic Medical Sciences, Department of Molecular Medicine, Oslo, NorwayHealth Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayHealth Effects Laboratory, Department of Environmental Chemistry, NILU—Norwegian Institute for Air Research, Kjeller, NorwayTo cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with the read-out or the reagent of the assay needs to be addressed to avoid biased results. Thus, validated label-free methods are urgently needed for hazard identification of NMs to avoid unintended adverse effects on human health. The colony forming efficiency (CFE) assay is a label- and interference-free method for quantification of cytotoxicity by cell survival and colony forming efficiency by CFE formation. The CFE has shown to be compatible with toxicity testing of NMs. Here we present an optimized protocol for a higher-throughput set up.https://www.frontiersin.org/articles/10.3389/ftox.2022.983316/fullnanomaterialscytotoxicitycell viabilitycolony formationCFE12-well format |
| spellingShingle | Elise Rundén-Pran Espen Mariussen Espen Mariussen Naouale El Yamani Elisabeth Elje Elisabeth Elje Eleonora Marta Longhin Maria Dusinska The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput Frontiers in Toxicology nanomaterials cytotoxicity cell viability colony formation CFE 12-well format |
| title | The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput |
| title_full | The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput |
| title_fullStr | The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput |
| title_full_unstemmed | The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput |
| title_short | The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput |
| title_sort | colony forming efficiency assay for toxicity testing of nanomaterials modifications for higher throughput |
| topic | nanomaterials cytotoxicity cell viability colony formation CFE 12-well format |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2022.983316/full |
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