Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks
Sporadic detection of live (viable) <i>Escherichia coli</i> in drinking water and biofilm with molecular methods but not with standard plate counts has raised concerns about the reliability of this indicator in the surveillance of drinking water safety. The aim of this study was to deter...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2013-04-01
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| Series: | Drinking Water Engineering and Science |
| Online Access: | http://www.drink-water-eng-sci.net/6/25/2013/dwes-6-25-2013.pdf |
| _version_ | 1818671962890698752 |
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| author | L. Mezule S. Larsson T. Juhna |
| author_facet | L. Mezule S. Larsson T. Juhna |
| author_sort | L. Mezule |
| collection | DOAJ |
| description | Sporadic detection of live (viable) <i>Escherichia coli</i> in drinking water and biofilm with molecular methods but not with standard plate counts has raised concerns about the reliability of this indicator in the surveillance of drinking water safety. The aim of this study was to determine spatial distribution of different viability forms of <i>E. coli</i> in a drinking water distribution system which complies with European Drinking Water Directive (98/83/EC). For two years coupons (two week old) and pre-concentrated (100 times with ultrafilters) water samples were collected after treatment plants and from four sites in the distribution network at several distances. The samples were analyzed for total, viable (able to divide as DVC-FISH positive) and cultivable <i>E. coli</i>. The results showed that low numbers of <i>E. coli</i> enters the distribution sytem from the treatment plants and tend to accumulate in the biofilm of water distribution system. Almost all of the samples contained metabolically active <i>E. coli</i> in the range of 1 to 50 cells per litre or cm<sup>2</sup> which represented approximately 53% of all <i>E. coli</i> detected. The amount of viable <i>E. coli</i> significantly increased into the network irrespective of the season. <br><br> The study has shown that DVC-FISH method in combination with water pre-concentration and biofilm sampling allows to better understand the behaviour of <i>E. coli</i> in water distribution networks, thus, it provides new evidences for water safety control. |
| first_indexed | 2024-12-17T07:32:20Z |
| format | Article |
| id | doaj.art-323fb4c9a2244b90b1f4285d4fcb7ba1 |
| institution | Directory Open Access Journal |
| issn | 1996-9457 1996-9465 |
| language | English |
| last_indexed | 2024-12-17T07:32:20Z |
| publishDate | 2013-04-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Drinking Water Engineering and Science |
| spelling | doaj.art-323fb4c9a2244b90b1f4285d4fcb7ba12022-12-21T21:58:26ZengCopernicus PublicationsDrinking Water Engineering and Science1996-94571996-94652013-04-0161253110.5194/dwes-6-25-2013Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networksL. MezuleS. LarssonT. JuhnaSporadic detection of live (viable) <i>Escherichia coli</i> in drinking water and biofilm with molecular methods but not with standard plate counts has raised concerns about the reliability of this indicator in the surveillance of drinking water safety. The aim of this study was to determine spatial distribution of different viability forms of <i>E. coli</i> in a drinking water distribution system which complies with European Drinking Water Directive (98/83/EC). For two years coupons (two week old) and pre-concentrated (100 times with ultrafilters) water samples were collected after treatment plants and from four sites in the distribution network at several distances. The samples were analyzed for total, viable (able to divide as DVC-FISH positive) and cultivable <i>E. coli</i>. The results showed that low numbers of <i>E. coli</i> enters the distribution sytem from the treatment plants and tend to accumulate in the biofilm of water distribution system. Almost all of the samples contained metabolically active <i>E. coli</i> in the range of 1 to 50 cells per litre or cm<sup>2</sup> which represented approximately 53% of all <i>E. coli</i> detected. The amount of viable <i>E. coli</i> significantly increased into the network irrespective of the season. <br><br> The study has shown that DVC-FISH method in combination with water pre-concentration and biofilm sampling allows to better understand the behaviour of <i>E. coli</i> in water distribution networks, thus, it provides new evidences for water safety control.http://www.drink-water-eng-sci.net/6/25/2013/dwes-6-25-2013.pdf |
| spellingShingle | L. Mezule S. Larsson T. Juhna Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks Drinking Water Engineering and Science |
| title | Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks |
| title_full | Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks |
| title_fullStr | Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks |
| title_full_unstemmed | Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks |
| title_short | Application of DVC-FISH method in tracking <i>Escherichia coli</i> in drinking water distribution networks |
| title_sort | application of dvc fish method in tracking i escherichia coli i in drinking water distribution networks |
| url | http://www.drink-water-eng-sci.net/6/25/2013/dwes-6-25-2013.pdf |
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