Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil
ABSTRACT Background: Leptospirosis represents a One Health issue, affecting humans and animals. This study investigated pathogenic leptospires in small wild rodents in São Paulo, Brazil. Methods: Kidney samples from 164 rodents underwent qPCR testing, targeting pathogenic Leptospira spp. Result...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2023-09-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100614&tlng=en |
| _version_ | 1797673977776177152 |
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| author | Evelyn Cristine da Silva Felipe Fornazari João Marcelo Azevedo de Paula Antunes Larissa de Castro Demoner Lucia Helena O’Dwyer de Oliveira Marina Gea Peres Jane Megid Helio Langoni |
| author_facet | Evelyn Cristine da Silva Felipe Fornazari João Marcelo Azevedo de Paula Antunes Larissa de Castro Demoner Lucia Helena O’Dwyer de Oliveira Marina Gea Peres Jane Megid Helio Langoni |
| author_sort | Evelyn Cristine da Silva |
| collection | DOAJ |
| description | ABSTRACT Background: Leptospirosis represents a One Health issue, affecting humans and animals. This study investigated pathogenic leptospires in small wild rodents in São Paulo, Brazil. Methods: Kidney samples from 164 rodents underwent qPCR testing, targeting pathogenic Leptospira spp. Results: Thirty-five animals (21.34%) tested positive, including five rodent species: Akodon montensis (2/21; 9.5%), Necromys lasiurus (1/4; 25%), Oligoryzomys nigripes (24/92; 26.1%), Oligoryzomys flavescens (5/26; 19.2%), and Sooretamys angouya (3/14; 21.4%). Botucatu municipality exhibited the highest prevalence, with 42.5% (20/47) of the animals testing positive. Conclusions: The presence of Leptospira spp. in wild rodents suggests they may be chronic carriers, contaminating the environment. |
| first_indexed | 2024-03-11T21:52:20Z |
| format | Article |
| id | doaj.art-324559b311504aeb861b22da3b7a4630 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-03-11T21:52:20Z |
| publishDate | 2023-09-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-324559b311504aeb861b22da3b7a46302023-09-26T07:40:39ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492023-09-015610.1590/0037-8682-0160-2023Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, BrazilEvelyn Cristine da Silvahttps://orcid.org/0000-0003-0722-7710Felipe Fornazarihttps://orcid.org/0000-0003-1995-7243João Marcelo Azevedo de Paula Antuneshttps://orcid.org/0000-0003-3922-1428Larissa de Castro Demonerhttps://orcid.org/0000-0003-0023-8046Lucia Helena O’Dwyer de Oliveirahttps://orcid.org/0000-0002-9367-368XMarina Gea Pereshttps://orcid.org/0000-0001-5788-9195Jane Megidhttps://orcid.org/0000-0002-6540-7157Helio Langonihttps://orcid.org/0000-0001-5127-0762ABSTRACT Background: Leptospirosis represents a One Health issue, affecting humans and animals. This study investigated pathogenic leptospires in small wild rodents in São Paulo, Brazil. Methods: Kidney samples from 164 rodents underwent qPCR testing, targeting pathogenic Leptospira spp. Results: Thirty-five animals (21.34%) tested positive, including five rodent species: Akodon montensis (2/21; 9.5%), Necromys lasiurus (1/4; 25%), Oligoryzomys nigripes (24/92; 26.1%), Oligoryzomys flavescens (5/26; 19.2%), and Sooretamys angouya (3/14; 21.4%). Botucatu municipality exhibited the highest prevalence, with 42.5% (20/47) of the animals testing positive. Conclusions: The presence of Leptospira spp. in wild rodents suggests they may be chronic carriers, contaminating the environment.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100614&tlng=enleptospirosisqPCRWildlifeRenal carrier |
| spellingShingle | Evelyn Cristine da Silva Felipe Fornazari João Marcelo Azevedo de Paula Antunes Larissa de Castro Demoner Lucia Helena O’Dwyer de Oliveira Marina Gea Peres Jane Megid Helio Langoni Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil Revista da Sociedade Brasileira de Medicina Tropical leptospirosis qPCR Wildlife Renal carrier |
| title | Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil |
| title_full | Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil |
| title_fullStr | Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil |
| title_full_unstemmed | Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil |
| title_short | Molecular detection of Leptospira spp. in small wild rodents from rural areas of São Paulo State, Brazil |
| title_sort | molecular detection of leptospira spp in small wild rodents from rural areas of sao paulo state brazil |
| topic | leptospirosis qPCR Wildlife Renal carrier |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100614&tlng=en |
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