Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide

IntroductionToxigenic Vibrio cholerae serogroup O1 and O139 are the pathogens responsible for the global cholera epidemic. V. cholerae can settle in the water and spread via the fecal-oral route. Rapid and accurate monitoring of live V. cholerae in environmental water has become an important strateg...

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Bibliographic Details
Main Authors: Jinsong Yang, Haibin Xu, Zili Ke, Naipeng Kan, Enhui Zheng, Yufeng Qiu, Mengying Huang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Microbiology
Subjects:
Vibrio cholerae
droplet digital PCR
propidium monoazide
multiplex detection
seawater
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1149981/full

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