MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor
The bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope. The flagellar motor is driven by an electrochemical gradient of cations such as H<sup>+</sup>, Na<sup>+</sup>, and K<sup>+</sup> through ion channels in stator complexes em...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/691 |
| _version_ | 1797569250889564160 |
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| author | Shun Naganawa Masahiro Ito |
| author_facet | Shun Naganawa Masahiro Ito |
| author_sort | Shun Naganawa |
| collection | DOAJ |
| description | The bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope. The flagellar motor is driven by an electrochemical gradient of cations such as H<sup>+</sup>, Na<sup>+</sup>, and K<sup>+</sup> through ion channels in stator complexes embedded in the cell membrane. The flagellum is believed to rotate as a result of electrostatic interaction forces between the stator and the rotor. In bacteria of the genus <i>Bacillus</i> and related species, the single transmembrane segment of MotB-type subunit protein (MotB and MotS) is critical for the selection of the H<sup>+</sup> and Na<sup>+</sup> coupling ions. Here, we constructed and characterized several hybrid stators combined with single Na<sup>+</sup>-coupled and dual Na<sup>+</sup>- and K<sup>+</sup>-coupled stator subunits, and we report that the MotP subunit is critical for the selection of K<sup>+</sup>. This result suggested that the K<sup>+</sup> selectivity of the MotP/MotS complexes evolved from the single Na<sup>+</sup>-coupled stator MotP/MotS complexes. This finding will promote the understanding of the evolution of flagellar motors and the molecular mechanisms of coupling ion selectivity. |
| first_indexed | 2024-03-10T20:09:17Z |
| format | Article |
| id | doaj.art-3247b15198114cd1a0737f21cc870947 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:09:17Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-3247b15198114cd1a0737f21cc8709472023-11-19T23:02:43ZengMDPI AGBiomolecules2218-273X2020-04-0110569110.3390/biom10050691MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar MotorShun Naganawa0Masahiro Ito1Graduate School of Life Sciences, Toyo University, Oura-gun, Gunma 374-0193, JapanGraduate School of Life Sciences, Toyo University, Oura-gun, Gunma 374-0193, JapanThe bacterial flagellar motor is a sophisticated nanomachine embedded in the cell envelope. The flagellar motor is driven by an electrochemical gradient of cations such as H<sup>+</sup>, Na<sup>+</sup>, and K<sup>+</sup> through ion channels in stator complexes embedded in the cell membrane. The flagellum is believed to rotate as a result of electrostatic interaction forces between the stator and the rotor. In bacteria of the genus <i>Bacillus</i> and related species, the single transmembrane segment of MotB-type subunit protein (MotB and MotS) is critical for the selection of the H<sup>+</sup> and Na<sup>+</sup> coupling ions. Here, we constructed and characterized several hybrid stators combined with single Na<sup>+</sup>-coupled and dual Na<sup>+</sup>- and K<sup>+</sup>-coupled stator subunits, and we report that the MotP subunit is critical for the selection of K<sup>+</sup>. This result suggested that the K<sup>+</sup> selectivity of the MotP/MotS complexes evolved from the single Na<sup>+</sup>-coupled stator MotP/MotS complexes. This finding will promote the understanding of the evolution of flagellar motors and the molecular mechanisms of coupling ion selectivity.https://www.mdpi.com/2218-273X/10/5/691<i>alkaliphiles</i>Mot complexpotassium ionflagellar motorevolution<i>Bacillus</i> |
| spellingShingle | Shun Naganawa Masahiro Ito MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor Biomolecules <i>alkaliphiles</i> Mot complex potassium ion flagellar motor evolution <i>Bacillus</i> |
| title | MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor |
| title_full | MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor |
| title_fullStr | MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor |
| title_full_unstemmed | MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor |
| title_short | MotP Subunit is Critical for Ion Selectivity and Evolution of a K<sup>+</sup>-Coupled Flagellar Motor |
| title_sort | motp subunit is critical for ion selectivity and evolution of a k sup sup coupled flagellar motor |
| topic | <i>alkaliphiles</i> Mot complex potassium ion flagellar motor evolution <i>Bacillus</i> |
| url | https://www.mdpi.com/2218-273X/10/5/691 |
| work_keys_str_mv | AT shunnaganawa motpsubunitiscriticalforionselectivityandevolutionofaksupsupcoupledflagellarmotor AT masahiroito motpsubunitiscriticalforionselectivityandevolutionofaksupsupcoupledflagellarmotor |