Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia
Flow cytometry (FCM) was implemented in 2008 at the Pontificia Universidad Javeriana and later at the Hospital Universitario San Ignacio to examine special samples of patients with hematological malignancies and solid tumors other than bone marrow and peripheral blood for diagnosis and monitoring. T...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2017-05-01
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| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/17956 |
| _version_ | 1819298201418596352 |
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| author | Alba Laura Derly Lina Eliana Graciela Antonia Iliana Martha Paula Carolina María Victoria Julio Dario Alejandra Felipe Juan Carlos Claudia Susana Sandra |
| author_facet | Alba Laura Derly Lina Eliana Graciela Antonia Iliana Martha Paula Carolina María Victoria Julio Dario Alejandra Felipe Juan Carlos Claudia Susana Sandra |
| author_sort | Alba |
| collection | DOAJ |
| description | Flow cytometry (FCM) was implemented in 2008 at the Pontificia Universidad Javeriana and later at the Hospital Universitario San Ignacio to examine special samples of patients with hematological malignancies and solid tumors other than bone marrow and peripheral blood for diagnosis and monitoring. This study describes the main findings of special sample evaluation over a six-year period. In all, 1070 samples of body fluids from patients with benign and malignant diseases were examined by FCM. These samples were stabilized with TransFixTM and stained with six-color immunophenotyping panels. Samples included cerebrospinal fluid, bronchoalveolar lavage, pleural fluid, pericardial fluid and ascite fluid from patients with acute and chronic leukemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumors, among others. Flow cytometry provided important information for the classification and detection of minimal numbers of tumor cells in leukemia and lymphoma cases. This work represents the first national report describing FCM implementation in special samples for diagnosis and clinical monitoring of patients with malignant and benign pathologies. |
| first_indexed | 2024-12-24T05:26:08Z |
| format | Article |
| id | doaj.art-3248efdedf7f4d66970986973b77fb67 |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-12-24T05:26:08Z |
| publishDate | 2017-05-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-3248efdedf7f4d66970986973b77fb672022-12-21T17:13:20ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522017-05-0122212314310.11144/Javeriana.SC22-2.sobfStudy of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - ColombiaAlba 0Laura 1Derly2Lina3Eliana 4Graciela5Antonia6Iliana 7Martha 8Paula Carolina9María Victoria10Julio11Dario12Alejandra13Felipe14Juan Carlos15Claudia16Susana 17Sandra 18CamposTrujilloLópezBeltránAriasVélezInfantede los ReyesVizcainoGuzmánHerreraSolanoLondoñoCañasPreteltPérezCardozoFiorentinoQuijanoFlow cytometry (FCM) was implemented in 2008 at the Pontificia Universidad Javeriana and later at the Hospital Universitario San Ignacio to examine special samples of patients with hematological malignancies and solid tumors other than bone marrow and peripheral blood for diagnosis and monitoring. This study describes the main findings of special sample evaluation over a six-year period. In all, 1070 samples of body fluids from patients with benign and malignant diseases were examined by FCM. These samples were stabilized with TransFixTM and stained with six-color immunophenotyping panels. Samples included cerebrospinal fluid, bronchoalveolar lavage, pleural fluid, pericardial fluid and ascite fluid from patients with acute and chronic leukemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumors, among others. Flow cytometry provided important information for the classification and detection of minimal numbers of tumor cells in leukemia and lymphoma cases. This work represents the first national report describing FCM implementation in special samples for diagnosis and clinical monitoring of patients with malignant and benign pathologies.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/17956body fluids;flow cytometry;immunophenotype |
| spellingShingle | Alba Laura Derly Lina Eliana Graciela Antonia Iliana Martha Paula Carolina María Victoria Julio Dario Alejandra Felipe Juan Carlos Claudia Susana Sandra Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia Universitas Scientiarum body fluids; flow cytometry; immunophenotype |
| title | Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia |
| title_full | Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia |
| title_fullStr | Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia |
| title_full_unstemmed | Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia |
| title_short | Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia |
| title_sort | study of body fluid samples using flow cytometry six years of experience at the hospital universitario san ignacio pontificia universidad javeriana bogota colombia |
| topic | body fluids; flow cytometry; immunophenotype |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/17956 |
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