Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells
Transfection is a contemporary approach for introducing foreign genetic material into target cells. The effective transport of genetic materials into cells is mostly influenced by (a) the characteristics of the genetic material (quantity and quality), (b) the transfection procedure (incubation time,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/12/626 |
| _version_ | 1797381582067073024 |
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| author | Hanaa Alkharobi |
| author_facet | Hanaa Alkharobi |
| author_sort | Hanaa Alkharobi |
| collection | DOAJ |
| description | Transfection is a contemporary approach for introducing foreign genetic material into target cells. The effective transport of genetic materials into cells is mostly influenced by (a) the characteristics of the genetic material (quantity and quality), (b) the transfection procedure (incubation time, ratio of the reagents to the introduced genetic material, and components of cell culture), and (c) targeted cells for transfection (cell origin and cell type). This review summarizes the findings of different studies focusing on various transfection approaches and their applications to explore the regenerative potential of dental pulp stem cells (DPSCs). Several databases, including Scopus, Google Scholar, and PubMed, were searched to obtain the literature for the current review. Different keywords were used as key terms in the search. Approximately 200 articles were retained after removing duplicates from different databases. Articles published in English that discussed different transfection approaches were included. Several sources were excluded because they did not meet the inclusion criteria. Approximately 70 relevant published sources were included in the final stage to achieve the study objectives. This review demonstrated that no single transfection system is applicable to all cases and the various cell types with no side effects. Further studies are needed to focus on optimizing process parameters, decreasing the toxicity and side effects of available transfection techniques, and increasing their efficiencies. Moreover, this review sheds light on the impact of using different valuable transfection approaches to investigate the regenerative potential of DPSCs. |
| first_indexed | 2024-03-08T20:53:29Z |
| format | Article |
| id | doaj.art-3249fea0bdcc43a4b603ecc07bb1db5f |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T20:53:29Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-3249fea0bdcc43a4b603ecc07bb1db5f2023-12-22T14:00:44ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-12-014512100261004010.3390/cimb45120626Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem CellsHanaa Alkharobi0Department of Oral Biology, College of Dentistry, King Abdul-Aziz University, Jeddah 21589, Saudi ArabiaTransfection is a contemporary approach for introducing foreign genetic material into target cells. The effective transport of genetic materials into cells is mostly influenced by (a) the characteristics of the genetic material (quantity and quality), (b) the transfection procedure (incubation time, ratio of the reagents to the introduced genetic material, and components of cell culture), and (c) targeted cells for transfection (cell origin and cell type). This review summarizes the findings of different studies focusing on various transfection approaches and their applications to explore the regenerative potential of dental pulp stem cells (DPSCs). Several databases, including Scopus, Google Scholar, and PubMed, were searched to obtain the literature for the current review. Different keywords were used as key terms in the search. Approximately 200 articles were retained after removing duplicates from different databases. Articles published in English that discussed different transfection approaches were included. Several sources were excluded because they did not meet the inclusion criteria. Approximately 70 relevant published sources were included in the final stage to achieve the study objectives. This review demonstrated that no single transfection system is applicable to all cases and the various cell types with no side effects. Further studies are needed to focus on optimizing process parameters, decreasing the toxicity and side effects of available transfection techniques, and increasing their efficiencies. Moreover, this review sheds light on the impact of using different valuable transfection approaches to investigate the regenerative potential of DPSCs.https://www.mdpi.com/1467-3045/45/12/626transfectiondental pulpstem cellregenerationdental stem cellregenerative medicine |
| spellingShingle | Hanaa Alkharobi Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells Current Issues in Molecular Biology transfection dental pulp stem cell regeneration dental stem cell regenerative medicine |
| title | Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells |
| title_full | Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells |
| title_fullStr | Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells |
| title_full_unstemmed | Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells |
| title_short | Exploring Various Transfection Approaches and Their Applications in Studying the Regenerative Potential of Dental Pulp Stem Cells |
| title_sort | exploring various transfection approaches and their applications in studying the regenerative potential of dental pulp stem cells |
| topic | transfection dental pulp stem cell regeneration dental stem cell regenerative medicine |
| url | https://www.mdpi.com/1467-3045/45/12/626 |
| work_keys_str_mv | AT hanaaalkharobi exploringvarioustransfectionapproachesandtheirapplicationsinstudyingtheregenerativepotentialofdentalpulpstemcells |