Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles
Fluorescence labeling of gold-aryl nanoparticles, AuNPs-COOH, was achieved by the covalent derivatization with dansyl chloride (DNS-Cl) reagent (5-naphthalene-1-sulfonyl chloride) for potential ssDNA recognition. The fluorescent gold nanoparticles of AuNPs-C<sub>6</sub>H<sub>4</...
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MDPI AG
2022-08-01
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| Series: | Colloids and Interfaces |
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| Online Access: | https://www.mdpi.com/2504-5377/6/3/42 |
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| author | Javad B. M. Parambath Gayathri A. Kanu Raed O. Abu Odeh Sanghyeon Kim Changseok Han Ahmed A. Mohamed |
| author_facet | Javad B. M. Parambath Gayathri A. Kanu Raed O. Abu Odeh Sanghyeon Kim Changseok Han Ahmed A. Mohamed |
| author_sort | Javad B. M. Parambath |
| collection | DOAJ |
| description | Fluorescence labeling of gold-aryl nanoparticles, AuNPs-COOH, was achieved by the covalent derivatization with dansyl chloride (DNS-Cl) reagent (5-naphthalene-1-sulfonyl chloride) for potential ssDNA recognition. The fluorescent gold nanoparticles of AuNPs-C<sub>6</sub>H<sub>4</sub>-4-COO-dansyl (AuNPs-DNS) of spherical shape and a size of 19.3 ± 8.3 nm were synthesized in a carbonate-bicarbonate buffer (pH = 10.6) at 37 °C. The fluorescence emission at 475 nm was acquired using fluorescence spectroscopy and investigated using time-resolved photoluminescence. The conjugation of ssDNA to AuNPs-DNS using the freeze-thaw and salt-aging methods was confirmed by fluorescence emission quenching, gel electrophoresis separation, and lifetime decrease. Conjugated ssDNA to AuNPs-DNS using the freeze-thaw method was more efficient than the salt-aging method. The purity of ssDNA upon conjugation was measured with optical density, and the obtained A<sub>260</sub>/A<sub>280</sub> ratio was in the range of 1.7–2.0. This research can be applied to other nucleotide recognition and theranostics. |
| first_indexed | 2024-03-10T00:21:37Z |
| format | Article |
| id | doaj.art-324ab2d205284e818e3b187ff918863b |
| institution | Directory Open Access Journal |
| issn | 2504-5377 |
| language | English |
| last_indexed | 2024-03-10T00:21:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Colloids and Interfaces |
| spelling | doaj.art-324ab2d205284e818e3b187ff918863b2023-11-23T15:41:12ZengMDPI AGColloids and Interfaces2504-53772022-08-01634210.3390/colloids6030042Single-Stranded DNA Recognition over Fluorescent Gold-Aryl NanoparticlesJavad B. M. Parambath0Gayathri A. Kanu1Raed O. Abu Odeh2Sanghyeon Kim3Changseok Han4Ahmed A. Mohamed5Department of Chemistry, College of Sciences, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, Sharjah 27272, United Arab EmiratesDepartment of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, Sharjah 27272, United Arab EmiratesProgram in Environmental & Polymer Engineering, Graduate School, INHA University, Incheon 22212, KoreaProgram in Environmental & Polymer Engineering, Graduate School, INHA University, Incheon 22212, KoreaDepartment of Chemistry, College of Sciences, University of Sharjah, Sharjah 27272, United Arab EmiratesFluorescence labeling of gold-aryl nanoparticles, AuNPs-COOH, was achieved by the covalent derivatization with dansyl chloride (DNS-Cl) reagent (5-naphthalene-1-sulfonyl chloride) for potential ssDNA recognition. The fluorescent gold nanoparticles of AuNPs-C<sub>6</sub>H<sub>4</sub>-4-COO-dansyl (AuNPs-DNS) of spherical shape and a size of 19.3 ± 8.3 nm were synthesized in a carbonate-bicarbonate buffer (pH = 10.6) at 37 °C. The fluorescence emission at 475 nm was acquired using fluorescence spectroscopy and investigated using time-resolved photoluminescence. The conjugation of ssDNA to AuNPs-DNS using the freeze-thaw and salt-aging methods was confirmed by fluorescence emission quenching, gel electrophoresis separation, and lifetime decrease. Conjugated ssDNA to AuNPs-DNS using the freeze-thaw method was more efficient than the salt-aging method. The purity of ssDNA upon conjugation was measured with optical density, and the obtained A<sub>260</sub>/A<sub>280</sub> ratio was in the range of 1.7–2.0. This research can be applied to other nucleotide recognition and theranostics.https://www.mdpi.com/2504-5377/6/3/42surface derivatizationfluorescent gold nanoparticlesaryldiazonium gold saltsdansyl chlorideDNA recognition |
| spellingShingle | Javad B. M. Parambath Gayathri A. Kanu Raed O. Abu Odeh Sanghyeon Kim Changseok Han Ahmed A. Mohamed Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles Colloids and Interfaces surface derivatization fluorescent gold nanoparticles aryldiazonium gold salts dansyl chloride DNA recognition |
| title | Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles |
| title_full | Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles |
| title_fullStr | Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles |
| title_full_unstemmed | Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles |
| title_short | Single-Stranded DNA Recognition over Fluorescent Gold-Aryl Nanoparticles |
| title_sort | single stranded dna recognition over fluorescent gold aryl nanoparticles |
| topic | surface derivatization fluorescent gold nanoparticles aryldiazonium gold salts dansyl chloride DNA recognition |
| url | https://www.mdpi.com/2504-5377/6/3/42 |
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