Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a major epidemic threat since the beginning of 2020. Efforts to combat the virus and the associated coronavirus disease 2019 (COVID-19) disease are being undertaken worldwide. To facilitate the research on the virus itself, a numbe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.732998/full |
| _version_ | 1818725143876206592 |
|---|---|
| author | Antonina Naskalska Agnieszka Dabrowska Agnieszka Dabrowska Artur Szczepanski Artur Szczepanski Krzysztof P. Jasik Beata Gromadzka Beata Gromadzka Krzysztof Pyrc |
| author_facet | Antonina Naskalska Agnieszka Dabrowska Agnieszka Dabrowska Artur Szczepanski Artur Szczepanski Krzysztof P. Jasik Beata Gromadzka Beata Gromadzka Krzysztof Pyrc |
| author_sort | Antonina Naskalska |
| collection | DOAJ |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a major epidemic threat since the beginning of 2020. Efforts to combat the virus and the associated coronavirus disease 2019 (COVID-19) disease are being undertaken worldwide. To facilitate the research on the virus itself, a number of surrogate systems have been developed. Here, we report the efficient production of SARS-CoV-2 virus-like particles (VLPs) in insect cells. Contrary to widely used pseudovirus particles, where only one coronaviral protein is displayed within a heterologous scaffold, developed VLPs are structurally similar to the native virus and allow for more throughput studies on the biology of the infection. On the other hand, being devoid of the viral genome, VLPs are unable to replicate and thus safe to work with. Importantly, this is the first report showing that SARS-CoV-2 VLPs can be efficiently produced in insect cells and purified using scalable affinity chromatography. |
| first_indexed | 2024-12-17T21:37:38Z |
| format | Article |
| id | doaj.art-324bb8faf15f465cb0ed5105d858088f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T21:37:38Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-324bb8faf15f465cb0ed5105d858088f2022-12-21T21:31:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-10-011210.3389/fmicb.2021.732998732998Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect CellsAntonina Naskalska0Agnieszka Dabrowska1Agnieszka Dabrowska2Artur Szczepanski3Artur Szczepanski4Krzysztof P. Jasik5Beata Gromadzka6Beata Gromadzka7Krzysztof Pyrc8Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, PolandVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, PolandVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, PolandDepartment of Pathology, School of Pharmacy, Medical University of Silesia in Katowice, Sosnowiec, PolandDepartment of “in vitro” Studies, Institute of Biotechnology and Molecular Medicine, Gdańsk, PolandNanoExpo®, Gdańsk, PolandVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, PolandSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a major epidemic threat since the beginning of 2020. Efforts to combat the virus and the associated coronavirus disease 2019 (COVID-19) disease are being undertaken worldwide. To facilitate the research on the virus itself, a number of surrogate systems have been developed. Here, we report the efficient production of SARS-CoV-2 virus-like particles (VLPs) in insect cells. Contrary to widely used pseudovirus particles, where only one coronaviral protein is displayed within a heterologous scaffold, developed VLPs are structurally similar to the native virus and allow for more throughput studies on the biology of the infection. On the other hand, being devoid of the viral genome, VLPs are unable to replicate and thus safe to work with. Importantly, this is the first report showing that SARS-CoV-2 VLPs can be efficiently produced in insect cells and purified using scalable affinity chromatography.https://www.frontiersin.org/articles/10.3389/fmicb.2021.732998/fullSARS CoV-2virus-like particlespurificationtransductioninsect cells |
| spellingShingle | Antonina Naskalska Agnieszka Dabrowska Agnieszka Dabrowska Artur Szczepanski Artur Szczepanski Krzysztof P. Jasik Beata Gromadzka Beata Gromadzka Krzysztof Pyrc Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells Frontiers in Microbiology SARS CoV-2 virus-like particles purification transduction insect cells |
| title | Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells |
| title_full | Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells |
| title_fullStr | Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells |
| title_full_unstemmed | Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells |
| title_short | Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells |
| title_sort | functional severe acute respiratory syndrome coronavirus 2 virus like particles from insect cells |
| topic | SARS CoV-2 virus-like particles purification transduction insect cells |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.732998/full |
| work_keys_str_mv | AT antoninanaskalska functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT agnieszkadabrowska functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT agnieszkadabrowska functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT arturszczepanski functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT arturszczepanski functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT krzysztofpjasik functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT beatagromadzka functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT beatagromadzka functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells AT krzysztofpyrc functionalsevereacuterespiratorysyndromecoronavirus2viruslikeparticlesfrominsectcells |