Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea
Background & aim: EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj. Meth...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Yasuj University Of Medical Sciences
2014-11-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-45&slc_lang=en&sid=1 |
| _version_ | 1811236361564323840 |
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| author | v Aein m Kargar a Doosti m Gholami |
| author_facet | v Aein m Kargar a Doosti m Gholami |
| author_sort | v Aein |
| collection | DOAJ |
| description | Background & aim: EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj.
Methods:This cross-sectional study was performed on 200 stool samples taken from children with diarrhea in Yasuj. After initial identification of E.coli strains by culture and biochemical tests, EIEC gene such as ipaH detected by PCR technicque and antibiotic susceptibility of isolates was evaluated by using disc diffusion (CLSI) method.
Results: Out of all examined samples, 16(8%) EIEC were separated. Antibiotic susceptibility test showed that the most susceptible antibiotic is ciprofloxacin for EIEC and also most resistant antibiotic is ceftizoxime.
Conclusion: Results showed that EIEC strains have a moderate prevalence than other studies in our study area. Therefore, for importance of this strain to producing dysentery, hospital-wide surveillance using molecular techniques hase been proposed in other regions of country. |
| first_indexed | 2024-04-12T12:07:47Z |
| format | Article |
| id | doaj.art-324d98361c7d415bada0ad24d9cfa81f |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T12:07:47Z |
| publishDate | 2014-11-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-324d98361c7d415bada0ad24d9cfa81f2022-12-22T03:33:40ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142014-11-01198727736Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrheav Aein0m Kargar1a Doosti2m Gholami3 Background & aim: EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj. Methods:This cross-sectional study was performed on 200 stool samples taken from children with diarrhea in Yasuj. After initial identification of E.coli strains by culture and biochemical tests, EIEC gene such as ipaH detected by PCR technicque and antibiotic susceptibility of isolates was evaluated by using disc diffusion (CLSI) method. Results: Out of all examined samples, 16(8%) EIEC were separated. Antibiotic susceptibility test showed that the most susceptible antibiotic is ciprofloxacin for EIEC and also most resistant antibiotic is ceftizoxime. Conclusion: Results showed that EIEC strains have a moderate prevalence than other studies in our study area. Therefore, for importance of this strain to producing dysentery, hospital-wide surveillance using molecular techniques hase been proposed in other regions of country.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-45&slc_lang=en&sid=1EnteroinvasiveE.coliEnteropathogen PCR Antibiotic resistance |
| spellingShingle | v Aein m Kargar a Doosti m Gholami Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea Armaghane Danesh Bimonthly Journal EnteroinvasiveE.coli Enteropathogen PCR Antibiotic resistance |
| title | Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea |
| title_full | Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea |
| title_fullStr | Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea |
| title_full_unstemmed | Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea |
| title_short | Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea |
| title_sort | detection of enteroinvasiveescherichia coli by pcr technique in children with diarrhea |
| topic | EnteroinvasiveE.coli Enteropathogen PCR Antibiotic resistance |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-45&slc_lang=en&sid=1 |
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